SNAP-tag^® and CLIP-tag™ Substrate Selection Chart

NEB^® offers a large selection of fluorescent labels (substrates) for SNAP- and CLIP-tag fusion proteins. SNAP-tag^® substrates consist of a fluorophore conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker, while CLIP-tag™ substrates consist of a fluorophore conjugated to a cytosine leaving group via a benzyl linker. These substrates will label their respective tags without the need for additional enzymes. Cell-permeable substrates (SNAP-Cell^® and CLIP-Cell™) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (SNAP-Surface^® and CLIP-Surface™) are specific for fusion proteins expressed on the cell surface only.