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RNA Modification

RNA can be modified with enzymes and reagents that act selectively depending on the existence of particular structures, and hence are useful tools for characterizing RNA species. The selective properties of RNA modifying enzymes enable researchers to distinguish different RNA species. Some unique end modifications can be used to selectively degrade or isolate particular RNA species when treated sequentially with the appropriate series of enzymes. For instance, 5´-capped RNAs are not substrates for polynucleotide kinases, or RNA ligases, but are substrates for decapping enzymes. Similarly, ligation and poly-adenylation of RNA 3´ end requires a free 3´.

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