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RNA Modification

RNA can be modified with enzymes and reagents that act selectively depending on the existence of particular structures, and hence are useful tools for characterizing RNA species. The selective properties of RNA modifying enzymes enable researchers to distinguish different RNA species. Some unique end modifications can be used to selectively degrade or isolate particular RNA species when treated sequentially with the appropriate series of enzymes. For instance, 5´-capped RNAs are not substrates for polynucleotide kinases, or RNA ligases, but are substrates for decapping enzymes. Similarly, ligation and poly-adenylation of RNA 3´ end requires a free 3´.

Protocols for RNA Modification

RNA Ligase Selection Chart

NEB offers a variety of ligases for RNA research with unique specificities.

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

  1. invitro_gaussia_video_tn

    In Vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

    This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene.