RNA Labeling

Detection of specific nucleic acid sequences can be accomplished by hybridization with a labeled RNA probe. RNA probes are sequences of variable lengths that are used to detect the presence of complementary nucleotide sequences in a sample. These RNA probes can be used for northern and southern blotting analyses, RNase protection assays, and in situ hybridization analyses. Probes can be generated by in vitro transcription, in which T7, T3 or SP6 RNA Polymerase is used to incorporate radioactive nucleotides or labeled modified nucleotides into the synthetic RNA, which can then be detected using radioactivity, fluorescence or chemiluminescence.

Choose Type:

Protocols for RNA Labeling
    Tools & Resources


  • Latest NEB Expressions

    This edition of NEB expressions discusses breakthroughs in Golden Gate Assembly research, that now enables 20+ fragment assembly with high efficiency and accuracy. New products include the NEBNext® Single Cell/Low Input RNA Library Prep Kit and the NEBNext Direct® Custom Ready Panels for NGS Target Enrichment. Lastly, learn about the importance of basic research at NEB in an interview with NEB’s Research Director.

  • RNA Technical Guide

    Find in depth information on tools designed to streamline your RNA workflows, including RNA extraction & purification, qPCR and RT-qPCR, RNA-seq, RNA synthesis and gRNA synthesis.

  • Selection Tools

  • Recommended HiScribe RNA Synthesis Kits by Application
  • Usage Guidelines

  • Avoiding Ribonuclease Contamination
RNA Polymerase Selection Chart
NEB offers RNA polymerases that can be used for in vitro synthesis of RNA for a wide variety of downstream applications. Additional polymerases are offered for the generation of untemplated homoribopolymeric tails for reverse transcription or labeling.
Legal Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.