Protein Analysis & Tools
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Protein Analysis & Tools includes these areas of focus:
FAQs for Protein Analysis & Tools
- What is Ph.D.™ Phage Display?
- Which residues does Endoproteinase AspN cut?
- Are there any amino acid residues that inhibit or reduce the efficiency of digestion of glutamate residues in a peptide sequence with Endoproteinase GluC? The site I want to digest has a glutamate residue followed by a proline residue and some enzymes are inhibited by the presence of a proline after the hydrolysis site.
- Is there a simple way to remove Trypsin after protein cleavage?
- How does one do a Trypsin in-gel digest?
- I have a very low concentration of protein and would prefer not to denature as a separate step with buffer exchange before digestion. What denaturants can he use in the Trypsin reaction itself?
- What is the Proteinase K activity in commonly used buffers?
Protocols for Protein Analysis & Tools
Application Notes for Protein Analysis & Tools
- Ph.D.™ Peptide Display Cloning System
- Simultaneous Fluorescent Labeling of Proteins in Living Cells
- Intein-Mediated Protein Ligation IPL and Labeling with the IMPACT™ Kit
- Analysis of a Fusion Protein using the Protein Deglycosylation Mix II and Mass Spectrometry
- Enzymatic removal of N- and O-glycans using PNGase F or the Protein Deglycosylation Mix
- Using Glycosidases to Remove, Trim, or Modify Glycans on Therapeutic Proteins
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Combating Neglected Diseases - a genomic approach to identify potential drug targets
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Parasitic Infections in Humans
Understand the common types of parasitic infections in humans and why they are still increasing in tropical regions.
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Applications of the Ph.D. Phage Display Peptide Libraries
- A monoclonal antibody for transcriptome-wide N6-methyladenosine analysis (2017)
- Crystal Structure of the 8 bp-Specific Restriction Enzyme SwaI (2015)
- NiCo21(DE3): A BL21(DE3) Derivative Designed for Expression and Purification of His-tagged Recombinant Protein (2015)
- Development of a Rapid Method for Genome Engineering in a Quest Towards Customized Protein Expression Strains (2015)
- Discovering the Maltose Binding Protein epitope tag for Escherichia coli expressed proteins (2018)
- Selection for proteins that overcome heatinduced lethality of ΔdegP strain (2018)
Tools & Resources
Feature Articles
Posters
- Leith, E.M., O'Dell, W.B., Ke, N., McClung, C., Berkmen, M., Bergonzo, C., Brinson, R.G., Kelman, Z (2019) Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli J Biol Chem; 294(48), 18046-18056.. PubMedID: 31604819, DOI: 10.1074/jbc.RA119.011008
- Ke, Na; Berkmen, Mehmet; Ren, Guoping; (2017) A water-soluble DsbB variant that catalyzes disulfide-bond formation in vivo Nat Chem Biol; 13, 1022-1028. PubMedID: 28628094, DOI: 10.1038/nchembio.2409
Publications related to Protein Analysis & Tools
Legal Information
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While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please email us at busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
