Learn more about NEB's extensive selection of DNA Ligases.
- Ligation Protocol with T4 DNA Ligase (M0202)
- Quick Ligation Protocol (M2200)
- Transformation Protocol
- Transformation Protocol (M0367)
- Transformation Protocol (M0370)
- Ligation Protocol for Cloning with Instant Sticky-end Ligase Master Mix (M0370)
- Ligation Protocol for Cloning with Blunt/TA Ligase Master Mix (M0367)
- Ligation Protocol for Cloning with ElectroLigase® (M0369)
- Transformation Protocol (M0369)
- Ligation Protocol with T3 DNA Ligase (M0317)
- Ligation Protocol with T7 DNA Ligase (M0318)
- Protocol for 9°N DNA Ligase (M0238)
- Protocol for Taq DNA Ligase (M0208)
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Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in three steps. Learn more about the function of ligation with our quick tutorial animation.
Ligation of blunt ends and single-base overhangs require optimized reaction conditions.
The optimal reactant ratio is contingent upon the downstream application.
Find out how the downstream application dictates the best reaction conditions for ligation.
Polyethylene glycol (PEG) is an important reagent in ligation reactions, find out why.