RNA Polymerase Selection Chart

T7 and SP6 RNA polymerases are DNA dependent RNA polymerases that produce DNA templated RNA transcripts. T7 and SP6 exhibit high specificity for their respective promoters. Both T7 and SP6 can be used for the in vitro synthesis of RNA for a wide variety of applications, including transfection, translation, structural studies and radioactive and non-isotopic probe generation. E. coli Poly(A) Polymerase and Poly(U) Polymerase generate untemplated homoribopolymeric tails on RNA 3´-ends. Both E. coli Poly(A) Polymerase and Poly(U) Polymerase can be used for RNA tailing prior to reverse transcription or for direct labeling.

Product NEB # Applications Size
T3 RNA Polymerase {EF668CB0-7C9A-1AB0-0670-21F45A6CE2A2}
  • Radiolabeled RNA probes
  • Non-isotopic RNA labeling
  • Preparation of RNA vaccines
  • Guide RNA for gene targeting
  • mRNA for in vitro translation and micro injection
  • RNA structure, processing and catalysis studies
  • RNA amplification
  • Anti-sense RNA for gene expression experiment
5000.0 units
T7 RNA Polymerase {CEBBA578-9F75-7177-FC4C-82FC3BF63AB6}
  • Radiolabeled RNA probes
  • Non-isotopic RNA labeling
  • Preparation of RNA vaccines
  • Guide RNA for gene targeting
  • mRNA for in vitro translation and micro injection 
  • RNA structure, processing and catalysis studies
  • RNA amplification
  • Anti-sense RNA for gene expression experiment 
5000.0 units
25000.0 units
Hi-T7® RNA Polymerase {59ECEDC5-66F9-5F0D-2F99-1E0930BD75DE}
  • Radiolabeled RNA probes
  • Non-isotopic RNA labeling
  • Guide RNA for gene targeting
  • mRNA for in vitro translation, microinjection and transfection 
  • RNA structure, processing and catalysis studies
  • RNA amplification
  • Isothermal amplification
  • Anti-sense RNA for gene expression
5000.0 units
SP6 RNA Polymerase {1B40ECE5-52A4-85D6-88DA-E184B4691827}
  • Radiolabeled RNA probe preparation
  • Non-isotopic RNA labeling
  • Preparation for RNA vaccines
  • Guide RNA for gene targeting
  • mRNA for in vitro translation and micro injection
  • RNA structure, processing and catalysis studies
  • RNA amplification
  • Anti-sense RNA for gene expression experiment
2000.0 units
10000.0 units
E. coli Poly(A) Polymerase {F95C40FB-940C-C1D2-5A58-771C88303B95}
  • Labeling of RNA with ATP or cordycepin
  • Poly(A) tailing of RNA for cloning or affinity purification
  • Enhances translation of RNA transferred into eukaryotic cells.
100.0 units
500.0 units
Poly(U) Polymerase {C1513F11-8F98-0700-B134-7872C29920C4}
  • Labeling of RNA with UTP
  • Poly(U) and Poly(A) tailing of RNA for cloning
  • Studying effects of poly(U) tailing on stability and translation of RNA transferred into eukaryotic cells
  • Poly (A) tailing of 2' O-Me modified 3' ends
60.0 units
E. coli RNA Polymerase, Core Enzyme {90B5B07B-8A55-D154-A095-224C780B566B}
  • RNA synthesis from E. coli promoter
  • Transcription initiation studies
  • In vitro translation with PURExpress
100.0 units
E. coli RNA Polymerase, Holoenzyme {4DA1C770-EC42-0DAE-EF8B-9BD34790AE20}
  • RNA synthesis from E. coli promoter 
  • Transcription initiation studies
  • In vitro translation with PURExpress
50.0 units
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