Isoschizomers

Restriction endonucleases that recognize the same sequence are isoschizomers. The first example discovered is called a prototype and all subsequent enzymes that recognize the same sequence are isoschizomers of the prototype. The list below contains isoschizomers for commercially available restriction endonucleases. It also specifies which isoschizomer is available from New England Biolabs.

All recognition sequences are written 5´ to 3´ using the single letter code nomenclature with the point of cleavage indicated by a "/".

Numbers in parentheses indicate point of cleavage for non-palindromic enzymes. For example, GGTCTC(1/5) indicates cleavage at:
5´ ...GGTCTCN/...3´
3´ ...CCAGAGNNNNN/...5´

Isoschizomers with alternative cleavage sites are indicated with a " ^ ". Enzymes that are not currently commercially available are indicated with a " x ".

Neoschizomers are a subset of isoschizomers that recognize the same sequence, but cleave at different positions from the prototype. Thus, AatII (recognition sequence: GACGT↓C) and ZraI (recognition sequence: GAC↓GTC) are neoschizomers of one another, while HpaII (recognition sequence: C↓CGG) and MspI (recognition sequence: C↓CGG) are isoschizomers. Analogous designations are not appropriate for MTases, where the differences between enzymes are not so easily defined and usually have not been well characterized.


(1) A HF version of this enzyme is available.
(2) A RE-Mix version of this enzyme is available.

Enzyme Sequence NEB Enzyme Catalog Number Cut Site Other Isoschizomers
AanI TTA/TAA PsiI R0657 TTA/TAA PsiI
AarI CACCTGC(4/8)
AasI GACNNNN/NNGTC DrdI R0530 GACNNNN/NNGTC DrdI, DseDI
AatII GACGT/C AatII R0117 GACGT/C ZraI^
ZraI^ R0659 GAC/GTC
Aba6411II x CRRTAAG
AbaCIII x CTATCAV
AbaSI C(11/9) AbaSI R0665 C(11/9)
AbaUMB2I x YCCGSS
AbsI CC/TCGAGG
Acc16I TGC/GCA FspI R0135 TGC/GCA FspI, NsbI
Acc36I ACCTGC(4/8) BfuAI R0701 ACCTGC(4/8) BfuAI, BspMI, BveI
BspMI R0502 ACCTGC(4/8)
Acc65I G/GTACC Acc65I R0599 G/GTACC Asp718I, KpnI^, KpnI-HF^
KpnI^ § R0142 GGTAC/C
KpnI-HF^ R3142 GGTAC/C
Acc65V x GACGCA
AccB1I G/GYRCC BanI R0118 G/GYRCC BanI, BshNI, BspT107I
AccB7I CCANNNN/NTGG PflMI R0509 CCANNNN/NTGG PflMI, Van91I
AccBSI CCGCTC(-3/-3) BsrBI R0102 CCGCTC(-3/-3) BsrBI, MbiI
AccI GT/MKAC AccI R0161 GT/MKAC FblI, XmiI
AccII CG/CG BstUI R0518 CG/CG Bsh1236I, BspFNI, BstFNI, BstUI, MvnI
AccIII T/CCGGA BspEI R0540 T/CCGGA Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I, MroI
AceIII x CAGCTC(7/11)
AchA6III x AGCCAG
AciI CCGC(-3/-1) AciI R0551 CCGC(-3/-1) BspACI, SsiI
AclI AA/CGTT AclI R0598 AA/CGTT Psp1406I
AclWI GGATC(4/5) AlwI R0513 GGATC(4/5) AlwI, BspPI
Aco12261II x CCRGAG
AcoI Y/GGCCR EaeI R0508 Y/GGCCR EaeI
AcsI R/AATTY ApoI § R0566 R/AATTY ApoI, ApoI-HF, XapI
ApoI-HF R3566 R/AATTY
AcuI CTGAAG(16/14) AcuI R0641 CTGAAG(16/14) Eco57I
AcvI CAC/GTG PmlI R0532 CAC/GTG BbrPI, Eco72I, PmaCI, PmlI, PspCI
AcyI GR/CGYC BsaHI R0556 GR/CGYC BsaHI, BssNI, BstACI, Hin1I, Hsp92I
AdeI CACNNN/GTG DraIII R3510 CACNNN/GTG DraIII-HF
DraIII-HF R3510 CACNNN/GTG
AfaI GT/AC CviQI^ R0639 G/TAC Csp6I^, CviQI^, RsaI, RsaNI^
RsaI R0167 GT/AC
AfeI AGC/GCT AfeI R0652 AGC/GCT Aor51HI, Eco47III
AfiI CCNNNNN/NNGG BslI R0555 CCNNNNN/NNGG Bsc4I, BseLI, BslI
AflII C/TTAAG AflII R0520 C/TTAAG BfrI, BspTI, BstAFI, MspCI, Vha464I
AflIII A/CRYGT AflIII R0541 A/CRYGT
AgeI A/CCGGT AgeI § R0552 A/CCGGT AgeI-HF, AsiGI, BshTI, CspAI, PinAI
AgeI-HF R3552 A/CCGGT
AgsI TTS/AA
AhaIII x TTT/AAA DraI R0129 TTT/AAA DraI
AhdI GACNNN/NNGTC AhdI R0584 GACNNN/NNGTC BmeRI, DriI, Eam1105I
AhlI A/CTAGT SpeI § R0133 A/CTAGT BcuI, SpeI, SpeI-HF
SpeI-HF R3133 A/CTAGT
AhyRBAHI x GCYYGAC
AhyYL17I x YAAMGAG
AjiI CACGTC(-3/-3) BmgBI R0628 CACGTC(-3/-3) BmgBI, BtrI
AjnI /CCWGG BstNI^ R0168 CC/WGG BciT130I^, BseBI^, BstNI^, Bst2UI^, EcoRII, MvaI^, Psp6I, PspGI
PspGI R0611 /CCWGG
AjuI (7/12)GAANNNNNNNTTGG(11/6)
AleI CACNN/NNGTG AleI R0634 CACNN/NNGTG OliI
AlfI x (10/12)GCANNNNNNTGC(12/10)
AloI (7/12)GAACNNNNNNTCC(12/7)
AluBI AG/CT AluI R0137 AG/CT AluI
AluI AG/CT AluI R0137 AG/CT AluBI
Alw21I GWGCW/C BsiHKAI R0570 GWGCW/C Bbv12I, BsiHKAI
Alw26I GTCTC(1/5) BcoDI R0542 GTCTC(1/5) BcoDI, BsmAI, BstMAI
BsmAI R0529 GTCTC(1/5)
Alw44I G/TGCAC ApaLI R0507 G/TGCAC ApaLI, VneI
AlwFI x GAAAYNNNNNRTG
AlwI GGATC(4/5) AlwI R0513 GGATC(4/5) AclWI, BspPI
AlwNI CAGNNN/CTG AlwNI R0514 CAGNNN/CTG CaiI, PstNI
Ama87I C/YCGRG AvaI R0152 C/YCGRG AvaI, BmeT110I, BsiHKCI, BsoBI, Eco88I
BsoBI R0586 C/YCGRG
AmaCSI x GCTCCA(11/9)
Aor13HI T/CCGGA BspEI R0540 T/CCGGA AccIII, BseAI, Bsp13I, BspEI, Kpn2I, MroI
Aor51HI AGC/GCT AfeI R0652 AGC/GCT AfeI, Eco47III
AoxI /GGCC
ApaBI x GCANNNNN/TGC BstAPI^ R0654 GCANNNN/NTGC BstAPI^
ApaI GGGCC/C ApaI R0114 GGGCC/C Bsp120I^, PspOMI^
PspOMI^ R0653 G/GGCCC
ApaLI G/TGCAC ApaLI R0507 G/TGCAC Alw44I, VneI
ApeKI G/CWGC ApeKI R0643 G/CWGC TseI
TseI R0591 G/CWGC
ApoI R/AATTY ApoI § R0566 R/AATTY AcsI, ApoI-HF, XapI
ApoI-HF R3566 R/AATTY
ApyPI x ATCGAC(20/18)
AquII x GCCGNAC(20/18)
AquIV x GRGGAAG(19/17)
ArsI (8/13)GACNNNNNNTTYG(11/6)
AscI GG/CGCGCC AscI R0558 GG/CGCGCC PalAI, SgsI
AseI AT/TAAT AseI R0526 AT/TAAT PshBI, VspI
AsiGI A/CCGGT AgeI § R0552 A/CCGGT AgeI, AgeI-HF, BshTI, CspAI, PinAI
AgeI-HF R3552 A/CCGGT
AsiSI GCGAT/CGC AsiSI R0630 GCGAT/CGC RgaI, SfaAI, SgfI
Asp114II x AGCABCC
Asp700I GAANN/NNTTC XmnI R0194 GAANN/NNTTC MroXI, PdmI, XmnI
Asp718I G/GTACC Acc65I R0599 G/GTACC Acc65I, KpnI^, KpnI-HF^
KpnI^ § R0142 GGTAC/C
KpnI-HF^ R3142 GGTAC/C
AspA2I C/CTAGG AvrII R0174 C/CTAGG AvrII, BlnI, XmaJI
AspBHI x YSCNS(8/12)
AspDUT2V x GNGCAAC
AspJHL3II x CGCCCAG
AspLEI GCG/C HhaI R0139 GCG/C BstHHI, CfoI, HhaI, Hin6I^, HinP1I^, HspAI^
HinP1I^ R0124 G/CGC
AspS9I G/GNCC Sau96I R0165 G/GNCC BmgT120I, Cfr13I, PspPI, Sau96I
Asu14238IV x CGTRAC
AsuC2I CC/SGG NciI R0196 CC/SGG BcnI, BpuMI, NciI
AsuHPI GGTGA(8/7) HphI R0158 GGTGA(8/7) HphI
AsuI x G/GNCC Sau96I R0165 G/GNCC AspS9I, BmgT120I, Cfr13I, PspPI, Sau96I
AsuII TT/CGAA BstBI R0519 TT/CGAA Bpu14I, Bsp119I, BspT104I, BstBI, NspV, SfuI
AsuNHI G/CTAGC BmtI^ § R0658 GCTAG/C BmtI^, BmtI-HF^, BspOI^, NheI, NheI-HF
BmtI-HF^ R3658 GCTAG/C
NheI § R0131 G/CTAGC
NheI-HF R3131 G/CTAGC
AteTI x GGGRAG
AvaI C/YCGRG AvaI R0152 C/YCGRG Ama87I, BmeT110I, BsiHKCI, BsoBI, Eco88I
BsoBI R0586 C/YCGRG
AvaII G/GWCC AvaII R0153 G/GWCC Bme18I, Eco47I, SinI, VpaK11BI
AvaIII x ATGCAT NsiI § R0127 ATGCA/T EcoT22I, Mph1103I, NsiI, NsiI-HF, Zsp2I
NsiI-HF R3127 ATGCA/T
AvrII C/CTAGG AvrII R0174 C/CTAGG AspA2I, BlnI, XmaJI
Awo1030IV x GCCRAG
AxyI CC/TNAGG Bsu36I R0524 CC/TNAGG Bse21I, Bsu36I, Eco81I
BaeGI GKGCM/C BaeGI R0708 GKGCM/C BseSI, BstSLI
BaeI (10/15)ACNNNNGTAYC(12/7) BaeI R0613 (10/15)ACNNNNGTAYC(12/7)
Bag18758I x CCCGAG
BalI TGG/CCA MscI R0534 TGG/CCA MlsI, MluNI, Mox20I, MscI, Msp20I
BamHI G/GATCC BamHI § R0136 G/GATCC BamHI-HF
BamHI-HF R3136 G/GATCC
BanI G/GYRCC BanI R0118 G/GYRCC AccB1I, BshNI, BspT107I
BanII GRGCY/C BanII R0119 GRGCY/C Eco24I, EcoT38I, FriOI
BanLI x RTCAGG
BarI (7/12)GAAGNNNNNNTAC(12/7)
BauI CACGAG(-5/-1) BssSI R0680 CACGAG(-5/-1) BssSI, Bst2BI
BbrPI CAC/GTG PmlI R0532 CAC/GTG AcvI, Eco72I, PmaCI, PmlI, PspCI
BbsI GAAGAC(2/6) BbsI § R0539 GAAGAC(2/6) BbsI-HF, BpiI, BstV2I
BbsI-HF R3539 GAAGAC(2/6)
Bbv12I GWGCW/C BsiHKAI R0570 GWGCW/C Alw21I, BsiHKAI
BbvCI CCTCAGC(-5/-2) BbvCI R0601 CCTCAGC(-5/-2)
BbvI GCAGC(8/12) BbvI R0173 GCAGC(8/12) BseXI, BstV1I, Lsp1109I
BbvII x GAAGAC(2/6) BbsI § R0539 GAAGAC(2/6) BbsI, BbsI-HF, BpiI, BstV2I
BbsI-HF R3539 GAAGAC(2/6)
BccI CCATC(4/5) BccI R0704 CCATC(4/5)
Bce3081I x TAGGAG
Bce83I x CTTGAG(16/14) BpuEI R0633 CTTGAG(16/14) BpuEI
BceAI ACGGC(12/14) BceAI R0623 ACGGC(12/14)
BcefI x ACGGC(12/13) BceAI^ R0623 ACGGC(12/14) BceAI^
BcgI (10/12)CGANNNNNNTGC(12/10) BcgI R0545 (10/12)CGANNNNNNTGC(12/10)
BciT130I CC/WGG BstNI R0168 CC/WGG AjnI^, BseBI, BstNI, Bst2UI, EcoRII^, MvaI, Psp6I^, PspGI^
PspGI^ R0611 /CCWGG
BciVI GTATCC(6/5) BciVI R0596 GTATCC(6/5) BfuI, BsuI
BclI T/GATCA BclI R0160 T/GATCA BclI-HF, FbaI, Ksp22I
BclI-HF R3160 T/GATCA
BcnI CC/SGG NciI R0196 CC/SGG AsuC2I, BpuMI, NciI
BcoDI GTCTC(1/5) BcoDI R0542 GTCTC(1/5) Alw26I, BsmAI, BstMAI
BsmAI R0529 GTCTC(1/5)
BcuI A/CTAGT SpeI § R0133 A/CTAGT AhlI, SpeI, SpeI-HF
SpeI-HF R3133 A/CTAGT
BdaI x (10/12)TGANNNNNNTCA(12/10)
BetI x W/CCGGW BsaWI R0567 W/CCGGW BsaWI
BfaI C/TAG BfaI R0568 C/TAG FspBI, MaeI, SspMI, XspI
BfaSII x GANGGAG
BfiI x ACTGGG(5/4) BmrI R0600 ACTGGG(5/4) BmrI, BmuI
BfmI C/TRYAG SfcI R0561 C/TRYAG BstSFI, SfcI
BfoI RGCGC/Y HaeII R0107 RGCGC/Y BstH2I, HaeII
BfrI C/TTAAG AflII R0520 C/TTAAG AflII, BspTI, BstAFI, MspCI, Vha464I
BfuAI ACCTGC(4/8) BfuAI R0701 ACCTGC(4/8) Acc36I, BspMI, BveI
BspMI R0502 ACCTGC(4/8)
BfuCI /GATC BfuCI R0636 /GATC Bsp143I, BssMI, BstKTI^, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI
DpnII R0543 /GATC
MboI R0147 /GATC
Sau3AI R0169 /GATC
BfuI GTATCC(6/5) BciVI R0596 GTATCC(6/5) BciVI, BsuI
BglI GCCNNNN/NGGC BglI R0143 GCCNNNN/NGGC
BglII A/GATCT BglII R0144 A/GATCT
BinI x GGATC(4/5) AlwI R0513 GGATC(4/5) AclWI, AlwI, BspPI
BisI GC/NGC BlsI^, GluI, PkrI^
Ble402II x GRAGCAG
BlnI C/CTAGG AvrII R0174 C/CTAGG AspA2I, AvrII, XmaJI
BloAII x GAGGAC
BlpI GC/TNAGC BlpI R0585 GC/TNAGC Bpu1102I, Bsp1720I
BlsI GCN/GC BisI^, GluI^, PkrI
BmcAI AGT/ACT ScaI R3122 AGT/ACT ScaI-HF, ZrmI
ScaI-HF R3122 AGT/ACT
Bme1390I CC/NGG ScrFI R0110 CC/NGG BmrFI, BstSCI^, MspR9I, ScrFI, StyD4I^
StyD4I^ R0638 /CCNGG
Bme18I G/GWCC AvaII R0153 G/GWCC AvaII, Eco47I, SinI, VpaK11BI
BmeRI GACNNN/NNGTC AhdI R0584 GACNNN/NNGTC AhdI, DriI, Eam1105I
BmeT110I C/YCGRG AvaI R0152 C/YCGRG Ama87I, AvaI, BsiHKCI, BsoBI, Eco88I
BsoBI R0586 C/YCGRG
BmgBI CACGTC(-3/-3) BmgBI R0628 CACGTC(-3/-3) AjiI, BtrI
BmgI x GKGCCC
BmgT120I G/GNCC Sau96I R0165 G/GNCC AspS9I, Cfr13I, PspPI, Sau96I
BmiI GGN/NCC NlaIV R0126 GGN/NCC BspLI, NlaIV, PspN4I
BmrFI CC/NGG ScrFI R0110 CC/NGG Bme1390I, BstSCI^, MspR9I, ScrFI, StyD4I^
StyD4I^ R0638 /CCNGG
BmrI ACTGGG(5/4) BmrI R0600 ACTGGG(5/4) BmuI
BmsI GCATC(5/9) SfaNI R0172 GCATC(5/9) LweI, SfaNI
BmtI GCTAG/C BmtI § R0658 GCTAG/C AsuNHI^, BmtI-HF, BspOI, NheI^, NheI-HF^
BmtI-HF R3658 GCTAG/C
NheI^ § R0131 G/CTAGC
NheI-HF^ R3131 G/CTAGC
BmuI ACTGGG(5/4) BmrI R0600 ACTGGG(5/4) BmrI
BoxI GACNN/NNGTC PshAI R0593 GACNN/NNGTC BstPAI, PshAI
BpiI GAAGAC(2/6) BbsI § R0539 GAAGAC(2/6) BbsI, BbsI-HF, BstV2I
BbsI-HF R3539 GAAGAC(2/6)
BplI (8/13)GAGNNNNNCTC(13/8)
BpmI CTGGAG(16/14) BpmI R0565 CTGGAG(16/14) GsuI
Bpu10I CCTNAGC(-5/-2) Bpu10I R0649 CCTNAGC(-5/-2)
Bpu1102I GC/TNAGC BlpI R0585 GC/TNAGC BlpI, Bsp1720I
Bpu14I TT/CGAA BstBI R0519 TT/CGAA AsuII, Bsp119I, BspT104I, BstBI, NspV, SfuI
BpuEI CTTGAG(16/14) BpuEI R0633 CTTGAG(16/14)
BpuMI CC/SGG NciI R0196 CC/SGG AsuC2I, BcnI, NciI
Bsa29I AT/CGAT BspDI R0557 AT/CGAT BseCI, BshVI, BspDI, Bsu15I, BsuTUI, ClaI
ClaI R0197 AT/CGAT
BsaAI YAC/GTR BsaAI R0531 YAC/GTR BstBAI, Ppu21I
BsaBI GATNN/NNATC BsaBI R0537 GATNN/NNATC Bse8I, BseJI
BsaHI GR/CGYC BsaHI R0556 GR/CGYC AcyI, BssNI, BstACI, Hin1I, Hsp92I
BsaI GGTCTC(1/5) BsaI § R0535 GGTCTC(1/5) BsaI-HF, Bso31I, BspTNI, Eco31I
BsaI-HF R3535 GGTCTC(1/5)
BsaJI C/CNNGG BsaJI R0536 C/CNNGG BseDI, BssECI
BsaWI W/CCGGW BsaWI R0567 W/CCGGW
BsaXI (9/12)ACNNNNNCTCC(10/7) BsaXI R0609 (9/12)ACNNNNNCTCC(10/7)
BsbI x CAACAC(21/19)
Bsc4I CCNNNNN/NNGG BslI R0555 CCNNNNN/NNGG AfiI, BseLI, BslI
BscGI x CCCGT
Bse118I R/CCGGY BsrFI R0682 R/CCGGY BsrFI, BssAI, Cfr10I
Bse1I ACTGG(1/-1) BsrI R0527 ACTGG(1/-1) BseNI, BsrI
Bse21I CC/TNAGG Bsu36I R0524 CC/TNAGG AxyI, Bsu36I, Eco81I
Bse3DI GCAATG(2/0) BsrDI R0574 GCAATG(2/0) BseMI, BsrDI
Bse8I GATNN/NNATC BsaBI R0537 GATNN/NNATC BsaBI, BseJI
BseAI T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI, Bsp13I, BspEI, Kpn2I, MroI
BseBI CC/WGG BstNI R0168 CC/WGG AjnI^, BciT130I, BstNI, Bst2UI, EcoRII^, MvaI, Psp6I^, PspGI^
PspGI^ R0611 /CCWGG
BseCI AT/CGAT BspDI R0557 AT/CGAT Bsa29I, BshVI, BspDI, Bsu15I, BsuTUI, ClaI
ClaI R0197 AT/CGAT
BseDI C/CNNGG BsaJI R0536 C/CNNGG BsaJI, BssECI
BseGI GGATG(2/0) BtsCI R0647 GGATG(2/0) BstF5I, BtsCI, FokI^
FokI^ R0109 GGATG(9/13)
BseJI GATNN/NNATC BsaBI R0537 GATNN/NNATC BsaBI, Bse8I
BseLI CCNNNNN/NNGG BslI R0555 CCNNNNN/NNGG AfiI, Bsc4I, BslI
BseMI GCAATG(2/0) BsrDI R0574 GCAATG(2/0) Bse3DI, BsrDI
BseMII CTCAG(10/8) BspCNI^ R0624 CTCAG(9/7) BspCNI^
BseNI ACTGG(1/-1) BsrI R0527 ACTGG(1/-1) Bse1I, BsrI
BsePI G/CGCGC BssHII R0199 G/CGCGC BssHII, PauI, PteI
BseRI GAGGAG(10/8) BseRI R0581 GAGGAG(10/8)
BseSI GKGCM/C BaeGI R0708 GKGCM/C BaeGI, BstSLI
BseX3I C/GGCCG EagI § R0505 C/GGCCG BstZI, EagI, EagI-HF, EclXI, Eco52I
EagI-HF R3505 C/GGCCG
BseXI GCAGC(8/12) BbvI R0173 GCAGC(8/12) BbvI, BstV1I, Lsp1109I
BseYI CCCAGC(-5/-1) BseYI R0635 CCCAGC(-5/-1) GsaI^, PspFI
BsgI GTGCAG(16/14) BsgI R0559 GTGCAG(16/14)
Bsh1236I CG/CG BstUI R0518 CG/CG AccII, BspFNI, BstFNI, BstUI, MvnI
Bsh1285I CGRY/CG BsiEI R0554 CGRY/CG BsiEI, BstMCI
BshFI GG/CC HaeIII R0108 GG/CC BsnI, BspANI, BsuRI, HaeIII
BshNI G/GYRCC BanI R0118 G/GYRCC AccB1I, BanI, BspT107I
BshTI A/CCGGT AgeI § R0552 A/CCGGT AgeI, AgeI-HF, AsiGI, CspAI, PinAI
AgeI-HF R3552 A/CCGGT
BshVI AT/CGAT BspDI R0557 AT/CGAT Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI
ClaI R0197 AT/CGAT
BsiEI CGRY/CG BsiEI R0554 CGRY/CG Bsh1285I, BstMCI
BsiHKAI GWGCW/C BsiHKAI R0570 GWGCW/C Alw21I, Bbv12I
BsiHKCI C/YCGRG AvaI R0152 C/YCGRG Ama87I, AvaI, BmeT110I, BsoBI, Eco88I
BsoBI R0586 C/YCGRG
BsiI x CACGAG(-5/-1) BssSI R0680 CACGAG(-5/-1) BauI, BssSI, Bst2BI
BsiSI C/CGG HpaII R0171 C/CGG HapII, HpaII, MspI
MspI R0106 C/CGG
BsiWI C/GTACG BsiWI § R0553 C/GTACG BsiWI-HF, Pfl23II, PspLI
BsiWI-HF R3553 C/GTACG
BsiYI x CCNNNNN/NNGG BslI R0555 CCNNNNN/NNGG AfiI, Bsc4I, BseLI, BslI
BslFI GGGAC(10/14) BsmFI R0572 GGGAC(10/14) BsmFI, FaqI
BslI CCNNNNN/NNGG BslI R0555 CCNNNNN/NNGG AfiI, Bsc4I, BseLI
BsmAI GTCTC(1/5) BcoDI R0542 GTCTC(1/5) Alw26I, BcoDI, BstMAI
BsmAI R0529 GTCTC(1/5)
BsmBI CGTCTC(1/5) BsmBI R0580 CGTCTC(1/5) Esp3I
BsmFI GGGAC(10/14) BsmFI R0572 GGGAC(10/14) BslFI, FaqI
BsmI GAATGC(1/-1) BsmI R0134 GAATGC(1/-1) Mva1269I, PctI
BsnI GG/CC HaeIII R0108 GG/CC BshFI, BspANI, BsuRI, HaeIII
Bso31I GGTCTC(1/5) BsaI § R0535 GGTCTC(1/5) BsaI, BsaI-HF, BspTNI, Eco31I
BsaI-HF R3535 GGTCTC(1/5)
BsoBI C/YCGRG AvaI R0152 C/YCGRG Ama87I, AvaI, BmeT110I, BsiHKCI, Eco88I
BsoBI R0586 C/YCGRG
Bsp119I TT/CGAA BstBI R0519 TT/CGAA AsuII, Bpu14I, BspT104I, BstBI, NspV, SfuI
Bsp120I G/GGCCC ApaI^ R0114 GGGCC/C ApaI^, PspOMI
PspOMI R0653 G/GGCCC
Bsp1286I GDGCH/C Bsp1286I R0120 GDGCH/C MhlI, SduI
Bsp13I T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI, BseAI, BspEI, Kpn2I, MroI
Bsp1407I T/GTACA BsrGI § R0575 T/GTACA BsrGI, BsrGI-HF, BstAUI
BsrGI-HF R3575 T/GTACA
Bsp143I /GATC BfuCI R0636 /GATC BfuCI, BssMI, BstKTI^, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI
DpnII R0543 /GATC
MboI R0147 /GATC
Sau3AI R0169 /GATC
Bsp1720I GC/TNAGC BlpI R0585 GC/TNAGC BlpI, Bpu1102I
Bsp19I C/CATGG NcoI § R0193 C/CATGG NcoI, NcoI-HF
NcoI-HF R3193 C/CATGG
Bsp24I x (8/13)GACNNNNNNTGG(12/7)
Bsp3004IV x CCGCAT
Bsp460III x CGCGCAG
Bsp68I TCG/CGA NruI § R0192 TCG/CGA BtuMI, NruI, NruI-HF, RruI
NruI-HF R3192 TCG/CGA
BspACI CCGC(-3/-1) AciI R0551 CCGC(-3/-1) AciI, SsiI
BspANI GG/CC HaeIII R0108 GG/CC BshFI, BsnI, BsuRI, HaeIII
BspCNI CTCAG(9/7) BspCNI R0624 CTCAG(9/7) BseMII^
BspDI AT/CGAT BspDI R0557 AT/CGAT Bsa29I, BseCI, BshVI, Bsu15I, BsuTUI, ClaI
ClaI R0197 AT/CGAT
BspEI T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI, BseAI, Bsp13I, Kpn2I, MroI
BspFNI CG/CG BstUI R0518 CG/CG AccII, Bsh1236I, BstFNI, BstUI, MvnI
BspGI x CTGGAC
BspHI T/CATGA BspHI R0517 T/CATGA CciI, PagI
BspLI GGN/NCC NlaIV R0126 GGN/NCC BmiI, NlaIV, PspN4I
BspLU11I x A/CATGT PciI R0655 A/CATGT PciI, PscI
BspMAI CTGCA/G PstI § R0140 CTGCA/G PstI, PstI-HF
PstI-HF R3140 CTGCA/G
BspMI ACCTGC(4/8) BfuAI R0701 ACCTGC(4/8) Acc36I, BfuAI, BveI
BspMI R0502 ACCTGC(4/8)
BspMII x T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I, MroI
BspNCI x CCAGA
BspOI GCTAG/C BmtI § R0658 GCTAG/C AsuNHI^, BmtI, BmtI-HF, NheI^, NheI-HF^
BmtI-HF R3658 GCTAG/C
NheI^ § R0131 G/CTAGC
NheI-HF^ R3131 G/CTAGC
BspPI GGATC(4/5) AlwI R0513 GGATC(4/5) AclWI, AlwI
BspQI GCTCTTC(1/4) BspQI R0712 GCTCTTC(1/4) LguI, PciSI, SapI
SapI R0569 GCTCTTC(1/4)
BspT104I TT/CGAA BstBI R0519 TT/CGAA AsuII, Bpu14I, Bsp119I, BstBI, NspV, SfuI
BspT107I G/GYRCC BanI R0118 G/GYRCC AccB1I, BanI, BshNI
BspTI C/TTAAG AflII R0520 C/TTAAG AflII, BfrI, BstAFI, MspCI, Vha464I
BspTNI GGTCTC(1/5) BsaI § R0535 GGTCTC(1/5) BsaI, BsaI-HF, Bso31I, Eco31I
BsaI-HF R3535 GGTCTC(1/5)
BsrBI CCGCTC(-3/-3) BsrBI R0102 CCGCTC(-3/-3) AccBSI, MbiI
BsrDI GCAATG(2/0) BsrDI R0574 GCAATG(2/0) Bse3DI, BseMI
BsrFI R/CCGGY BsrFI R0682 R/CCGGY Bse118I, BssAI, Cfr10I
BsrGI T/GTACA BsrGI § R0575 T/GTACA Bsp1407I, BsrGI-HF, BstAUI
BsrGI-HF R3575 T/GTACA
BsrI ACTGG(1/-1) BsrI R0527 ACTGG(1/-1) Bse1I, BseNI
BssAI R/CCGGY BsrFI R0682 R/CCGGY Bse118I, BsrFI, Cfr10I
BssECI C/CNNGG BsaJI R0536 C/CNNGG BsaJI, BseDI
BssHII G/CGCGC BssHII R0199 G/CGCGC BsePI, PauI, PteI
BssMI /GATC BfuCI R0636 /GATC BfuCI, Bsp143I, BstKTI^, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI
DpnII R0543 /GATC
MboI R0147 /GATC
Sau3AI R0169 /GATC
BssNAI GTA/TAC BstZ17I R3594 GTA/TAC Bst1107I, BstZ17I-HF
BstZ17I-HF R3594 GTA/TAC
BssNI GR/CGYC BsaHI R0556 GR/CGYC AcyI, BsaHI, BstACI, Hin1I, Hsp92I
BssSI CACGAG(-5/-1) BssSI R0680 CACGAG(-5/-1) BauI, Bst2BI
BssT1I C/CWWGG StyI § R0500 C/CWWGG Eco130I, EcoT14I, ErhI, StyI, StyI-HF
StyI-HF R3500 C/CWWGG
Bst1107I GTA/TAC BstZ17I R3594 GTA/TAC BssNAI, BstZ17I-HF
BstZ17I-HF R3594 GTA/TAC
Bst2BI CACGAG(-5/-1) BssSI R0680 CACGAG(-5/-1) BauI, BssSI
Bst2UI CC/WGG BstNI R0168 CC/WGG AjnI^, BciT130I, BseBI, BstNI, EcoRII^, MvaI, Psp6I^, PspGI^
PspGI^ R0611 /CCWGG
Bst4CI ACN/GT HpyCH4III R0618 ACN/GT HpyCH4III, TaaI
Bst6I CTCTTC(1/4) EarI R0528 CTCTTC(1/4) Eam1104I, EarI
BstACI GR/CGYC BsaHI R0556 GR/CGYC AcyI, BsaHI, BssNI, Hin1I, Hsp92I
BstAFI C/TTAAG AflII R0520 C/TTAAG AflII, BfrI, BspTI, MspCI, Vha464I
BstAPI GCANNNN/NTGC BstAPI R0654 GCANNNN/NTGC
BstAUI T/GTACA BsrGI § R0575 T/GTACA Bsp1407I, BsrGI, BsrGI-HF
BsrGI-HF R3575 T/GTACA
BstBAI YAC/GTR BsaAI R0531 YAC/GTR BsaAI, Ppu21I
BstBI TT/CGAA BstBI R0519 TT/CGAA AsuII, Bpu14I, Bsp119I, BspT104I, NspV, SfuI
BstC8I GCN/NGC Cac8I R0579 GCN/NGC Cac8I
BstDEI C/TNAG DdeI R0175 C/TNAG DdeI, HpyF3I
BstDSI C/CRYGG BtgI R0608 C/CRYGG BtgI
BstEII G/GTNACC BstEII § R0162 G/GTNACC BstEII-HF, BstPI, Eco91I, EcoO65I, PspEI
BstEII-HF R3162 G/GTNACC
BstENI CCTNN/NNNAGG EcoNI R0521 CCTNN/NNNAGG EcoNI, XagI
BstF5I GGATG(2/0) BtsCI R0647 GGATG(2/0) BseGI, BtsCI, FokI^
FokI^ R0109 GGATG(9/13)
BstFNI CG/CG BstUI R0518 CG/CG AccII, Bsh1236I, BspFNI, BstUI, MvnI
BstH2I RGCGC/Y HaeII R0107 RGCGC/Y BfoI, HaeII
BstHHI GCG/C HhaI R0139 GCG/C AspLEI, CfoI, HhaI, Hin6I^, HinP1I^, HspAI^
HinP1I^ R0124 G/CGC
BstKTI GAT/C BfuCI^ R0636 /GATC BfuCI^, Bsp143I^, BssMI^, BstMBI^, DpnII^, Kzo9I^, MboI^, NdeII^, Sau3AI^
DpnII^ R0543 /GATC
MboI^ R0147 /GATC
Sau3AI^ R0169 /GATC
BstMAI GTCTC(1/5) BcoDI R0542 GTCTC(1/5) Alw26I, BcoDI, BsmAI
BsmAI R0529 GTCTC(1/5)
BstMBI /GATC BfuCI R0636 /GATC BfuCI, Bsp143I, BssMI, BstKTI^, DpnII, Kzo9I, MboI, NdeII, Sau3AI
DpnII R0543 /GATC
MboI R0147 /GATC
Sau3AI R0169 /GATC
BstMCI CGRY/CG BsiEI R0554 CGRY/CG Bsh1285I, BsiEI
BstMWI GCNNNNN/NNGC MwoI R0573 GCNNNNN/NNGC HpyF10VI, MwoI
BstNI CC/WGG BstNI R0168 CC/WGG AjnI^, BciT130I, BseBI, Bst2UI, EcoRII^, MvaI, Psp6I^, PspGI^
PspGI^ R0611 /CCWGG
BstNSI RCATG/Y NspI R0602 RCATG/Y NspI, XceI
BstPAI GACNN/NNGTC PshAI R0593 GACNN/NNGTC BoxI, PshAI
BstPI G/GTNACC BstEII § R0162 G/GTNACC BstEII, BstEII-HF, Eco91I, EcoO65I, PspEI
BstEII-HF R3162 G/GTNACC
BstSCI /CCNGG ScrFI^ R0110 CC/NGG Bme1390I^, BmrFI^, MspR9I^, ScrFI^, StyD4I
StyD4I R0638 /CCNGG
BstSFI C/TRYAG SfcI R0561 C/TRYAG BfmI, SfcI
BstSLI GKGCM/C BaeGI R0708 GKGCM/C BaeGI, BseSI
BstSNI TAC/GTA SnaBI R0130 TAC/GTA Eco105I, SnaBI
BstUI CG/CG BstUI R0518 CG/CG AccII, Bsh1236I, BspFNI, BstFNI, MvnI
BstV1I GCAGC(8/12) BbvI R0173 GCAGC(8/12) BbvI, BseXI, Lsp1109I
BstV2I GAAGAC(2/6) BbsI § R0539 GAAGAC(2/6) BbsI, BbsI-HF, BpiI
BbsI-HF R3539 GAAGAC(2/6)
BstX2I R/GATCY BstYI R0523 R/GATCY BstYI, MflI, PsuI
BstXI CCANNNNN/NTGG BstXI R0113 CCANNNNN/NTGG
BstYI R/GATCY BstYI R0523 R/GATCY BstX2I, MflI, PsuI
BstZ17I-HF GTA/TAC BstZ17I-HF R3594 GTA/TAC BssNAI, Bst1107I, BstZ17I-HF
BstZI C/GGCCG EagI § R0505 C/GGCCG BseX3I, EagI, EagI-HF, EclXI, Eco52I
EagI-HF R3505 C/GGCCG
Bsu15I AT/CGAT BspDI R0557 AT/CGAT Bsa29I, BseCI, BshVI, BspDI, BsuTUI, ClaI
ClaI R0197 AT/CGAT
Bsu36I CC/TNAGG Bsu36I R0524 CC/TNAGG AxyI, Bse21I, Eco81I
BsuI GTATCC(6/5) BciVI R0596 GTATCC(6/5) BciVI, BfuI
BsuRI GG/CC HaeIII R0108 GG/CC BshFI, BsnI, BspANI, HaeIII
BsuTUI AT/CGAT BspDI R0557 AT/CGAT Bsa29I, BseCI, BshVI, BspDI, Bsu15I, ClaI
ClaI R0197 AT/CGAT
BtgI C/CRYGG BtgI R0608 C/CRYGG BstDSI
BtgZI GCGATG(10/14) BtgZI R0703 GCGATG(10/14)
BtrI CACGTC(-3/-3) BmgBI R0628 CACGTC(-3/-3) AjiI, BmgBI
BtsCI GGATG(2/0) BtsCI R0647 GGATG(2/0) BseGI, BstF5I, FokI^
FokI^ R0109 GGATG(9/13)
BtsI GCAGTG(2/0) BtsI R0667 GCAGTG(2/0)
BtsIMutI CAGTG(2/0) BtsIMutI R0664 CAGTG(2/0)
BtuMI TCG/CGA NruI § R0192 TCG/CGA Bsp68I, NruI, NruI-HF, RruI
NruI-HF R3192 TCG/CGA
BveI ACCTGC(4/8) BfuAI R0701 ACCTGC(4/8) Acc36I, BfuAI, BspMI
BspMI R0502 ACCTGC(4/8)
Cac8I GCN/NGC Cac8I R0579 GCN/NGC BstC8I
CaiI CAGNNN/CTG AlwNI R0514 CAGNNN/CTG AlwNI, PstNI
Cal14237I x GGTTAG
CalB3II x GRTTRAG
Cau10061II x GTTAAT
CauII x CC/SGG NciI R0196 CC/SGG AsuC2I, BcnI, BpuMI, NciI
Cba13II x AGGAAT
Cba16038I x CCTNAYNC
CchII x GGARGA(11/9)
CchIII x CCCAAG(20/18)
CciI T/CATGA BspHI R0517 T/CATGA BspHI, PagI
CciNI GC/GGCCGC NotI § R0189 GC/GGCCGC NotI, NotI-HF
NotI-HF R3189 GC/GGCCGC
CcrNAIII x CGACCAG
Cdi11397I x GCGCAG
Cdi81III x GCMGAAG
CdiI x CATCG(-1/-1)
CdpI x GCGGAG(20/18)
Cdu23823II x GTGAAG
CfoI GCG/C HhaI R0139 GCG/C AspLEI, BstHHI, HhaI, Hin6I^, HinP1I^, HspAI^
HinP1I^ R0124 G/CGC
Cfr10I R/CCGGY BsrFI R0682 R/CCGGY Bse118I, BsrFI, BssAI
Cfr13I G/GNCC Sau96I R0165 G/GNCC AspS9I, BmgT120I, PspPI, Sau96I
Cfr42I CCGC/GG SacII R0157 CCGC/GG KspI, SacII, Sfr303I, SgrBI
Cfr9I C/CCGGG SmaI^ R0141 CCC/GGG SmaI^, TspMI, XmaI
TspMI R0709 C/CCGGG
XmaI R0180 C/CCGGG
CfrI x Y/GGCCR EaeI R0508 Y/GGCCR AcoI, EaeI
Cfupf3II x GARCAG
Cgl13032I x GGCGCA
Cgl13032II x ACGABGG
CjeFIII x GCAAGG
CjeFV x GGRCA
CjeI x (8/14)CCANNNNNNGT(15/9)
CjeNII x GAGNNNNNGT
CjeNIII x GKAAYG(19/17)
CjeNV x CCYGA
CjeP659IV x CACNNNNNNNGAA
CjePI x (7/13)CCANNNNNNNTC(14/8)
CjuI x CAYNNNNNRTG
CjuII x CAYNNNNNCTC
Cla11845III x GCGAA
ClaI AT/CGAT BspDI R0557 AT/CGAT Bsa29I, BseCI, BshVI, BspDI, Bsu15I, BsuTUI
ClaI R0197 AT/CGAT
Cly7489II x AAAAGRG
Cma23826I x CGGAAG
CpoI CG/GWCCG RsrII R0501 CG/GWCCG CspI, RsrII, Rsr2I
CseI GACGC(5/10) HgaI R0154 GACGC(5/10) HgaI
CsiI A/CCWGGT SexAI R0605 A/CCWGGT MabI, SexAI
Csp2014I x GGAGGC
Csp6I G/TAC CviQI R0639 G/TAC AfaI^, CviQI, RsaI^, RsaNI
RsaI^ R0167 GT/AC
CspAI A/CCGGT AgeI § R0552 A/CCGGT AgeI, AgeI-HF, AsiGI, BshTI, PinAI
AgeI-HF R3552 A/CCGGT
CspCI (11/13)CAANNNNNGTGG(12/10) CspCI R0645 (11/13)CAANNNNNGTGG(12/10)
CspI CG/GWCCG RsrII R0501 CG/GWCCG CpoI, RsrII, Rsr2I
CstMI x AAGGAG(20/18)
CviAII C/ATG CviAII R0640 C/ATG FaeI^, FatI^^, Hin1II^, Hsp92II^, NlaIII^
FatI^^ R0650 /CATG
NlaIII^ R0125 CATG/
CviJI RG/CY CviKI-1 R0710 RG/CY CviKI-1
CviKI-1 RG/CY CviKI-1 R0710 RG/CY CviJI
CviQI G/TAC CviQI R0639 G/TAC AfaI^, Csp6I, RsaI^, RsaNI
RsaI^ R0167 GT/AC
CviRI x TG/CA HpyCH4V R0620 TG/CA HpyCH4V
Dde51507I x CCWGG
DdeI C/TNAG DdeI R0175 C/TNAG BstDEI, HpyF3I
DinI GGC/GCC KasI^ R0544 G/GCGCC EgeI, EheI, KasI^, Mly113I^^, NarI^^, PluTI^^^, SfoI, SspDI^
NarI^^ R0191 GG/CGCC
PluTI^^^ R0713 GGCGC/C
SfoI R0606 GGC/GCC
DpnI GA/TC DpnI R0176 GA/TC MalI
DpnII /GATC BfuCI R0636 /GATC BfuCI, Bsp143I, BssMI, BstKTI^, BstMBI, Kzo9I, MboI, NdeII, Sau3AI
DpnII R0543 /GATC
MboI R0147 /GATC
Sau3AI R0169 /GATC
DraI TTT/AAA DraI R0129 TTT/AAA
DraII x RG/GNCCY EcoO109I R0503 RG/GNCCY EcoO109I
DraRI x CAAGNAC(20/18)
DrdI GACNNNN/NNGTC DrdI R0530 GACNNNN/NNGTC AasI, DseDI
DrdII x GAACCA
DriI GACNNN/NNGTC AhdI R0584 GACNNN/NNGTC AhdI, BmeRI, Eam1105I
DsaI x C/CRYGG BtgI R0608 C/CRYGG BstDSI, BtgI
DseDI GACNNNN/NNGTC DrdI R0530 GACNNNN/NNGTC AasI, DrdI
EaeI Y/GGCCR EaeI R0508 Y/GGCCR AcoI
EagI C/GGCCG EagI § R0505 C/GGCCG BseX3I, BstZI, EagI-HF, EclXI, Eco52I
EagI-HF R3505 C/GGCCG
Eam1104I CTCTTC(1/4) EarI R0528 CTCTTC(1/4) Bst6I, EarI
Eam1105I GACNNN/NNGTC AhdI R0584 GACNNN/NNGTC AhdI, BmeRI, DriI
EarI CTCTTC(1/4) EarI R0528 CTCTTC(1/4) Bst6I, Eam1104I
EciI GGCGGA(11/9) EciI R0590 GGCGGA(11/9)
Ecl136II GAG/CTC Eco53kI R0116 GAG/CTC EcoICRI, Eco53kI, Psp124BI^, SacI^, SacI-HF^, SstI^
SacI^ § R0156 GAGCT/C
SacI-HF^ R3156 GAGCT/C
Ecl35734I x GAAAYTC
EclXI C/GGCCG EagI § R0505 C/GGCCG BseX3I, BstZI, EagI, EagI-HF, Eco52I
EagI-HF R3505 C/GGCCG
Eco105I TAC/GTA SnaBI R0130 TAC/GTA BstSNI, SnaBI
Eco130I C/CWWGG StyI § R0500 C/CWWGG BssT1I, EcoT14I, ErhI, StyI, StyI-HF
StyI-HF R3500 C/CWWGG
Eco147I AGG/CCT StuI R0187 AGG/CCT PceI, SseBI, StuI
Eco24I GRGCY/C BanII R0119 GRGCY/C BanII, EcoT38I, FriOI
Eco31I GGTCTC(1/5) BsaI § R0535 GGTCTC(1/5) BsaI, BsaI-HF, Bso31I, BspTNI
BsaI-HF R3535 GGTCTC(1/5)
Eco32I GAT/ATC EcoRV § R0195 GAT/ATC EcoRV, EcoRV-HF
EcoRV-HF R3195 GAT/ATC
Eco4465II x GAAABCC
Eco47I G/GWCC AvaII R0153 G/GWCC AvaII, Bme18I, SinI, VpaK11BI
Eco47III AGC/GCT AfeI R0652 AGC/GCT AfeI, Aor51HI
Eco52I C/GGCCG EagI § R0505 C/GGCCG BseX3I, BstZI, EagI, EagI-HF, EclXI
EagI-HF R3505 C/GGCCG
Eco53kI GAG/CTC Eco53kI R0116 GAG/CTC Ecl136II, EcoICRI, Psp124BI^, SacI^, SacI-HF^, SstI^
SacI^ § R0156 GAGCT/C
SacI-HF^ R3156 GAGCT/C
Eco57I CTGAAG(16/14) AcuI R0641 CTGAAG(16/14) AcuI
Eco57MI x CTGRAG(16/14)
Eco72I CAC/GTG PmlI R0532 CAC/GTG AcvI, BbrPI, PmaCI, PmlI, PspCI
Eco81I CC/TNAGG Bsu36I R0524 CC/TNAGG AxyI, Bse21I, Bsu36I
Eco88I C/YCGRG AvaI R0152 C/YCGRG Ama87I, AvaI, BmeT110I, BsiHKCI, BsoBI
BsoBI R0586 C/YCGRG
Eco91I G/GTNACC BstEII § R0162 G/GTNACC BstEII, BstEII-HF, BstPI, EcoO65I, PspEI
BstEII-HF R3162 G/GTNACC
EcoBLMcrX x RCSRC(-3/-2)
EcoE1140I x ACCYAC
EcoICRI GAG/CTC Eco53kI R0116 GAG/CTC Ecl136II, Eco53kI, Psp124BI^, SacI^, SacI-HF^, SstI^
SacI^ § R0156 GAGCT/C
SacI-HF^ R3156 GAGCT/C
EcoMVII x CANCATC
EcoNI CCTNN/NNNAGG EcoNI R0521 CCTNN/NNNAGG BstENI, XagI
EcoO109I RG/GNCCY EcoO109I R0503 RG/GNCCY
EcoO65I G/GTNACC BstEII § R0162 G/GTNACC BstEII, BstEII-HF, BstPI, Eco91I, PspEI
BstEII-HF R3162 G/GTNACC
EcoRI G/AATTC EcoRI § R0101 G/AATTC EcoRI-HF
EcoRI-HF R3101 G/AATTC
EcoRII /CCWGG BstNI^ R0168 CC/WGG AjnI, BciT130I^, BseBI^, BstNI^, Bst2UI^, MvaI^, Psp6I, PspGI
PspGI R0611 /CCWGG
EcoRV GAT/ATC EcoRV § R0195 GAT/ATC Eco32I, EcoRV-HF
EcoRV-HF R3195 GAT/ATC
EcoT14I C/CWWGG StyI § R0500 C/CWWGG BssT1I, Eco130I, ErhI, StyI, StyI-HF
StyI-HF R3500 C/CWWGG
EcoT22I ATGCA/T NsiI § R0127 ATGCA/T Mph1103I, NsiI, NsiI-HF, Zsp2I
NsiI-HF R3127 ATGCA/T
EcoT38I GRGCY/C BanII R0119 GRGCY/C BanII, Eco24I, FriOI
EgeI GGC/GCC KasI^ R0544 G/GCGCC DinI, EheI, KasI^, Mly113I^^, NarI^^, PluTI^^^, SfoI, SspDI^
NarI^^ R0191 GG/CGCC
PluTI^^^ R0713 GGCGC/C
SfoI R0606 GGC/GCC
EheI GGC/GCC KasI^ R0544 G/GCGCC DinI, EgeI, KasI^, Mly113I^^, NarI^^, PluTI^^^, SfoI, SspDI^
NarI^^ R0191 GG/CGCC
PluTI^^^ R0713 GGCGC/C
SfoI R0606 GGC/GCC
Eli8509II x CCGGAG
ErhI C/CWWGG StyI § R0500 C/CWWGG BssT1I, Eco130I, EcoT14I, StyI, StyI-HF
StyI-HF R3500 C/CWWGG
EsaSSI x GACCAC
Esp3007I x CAGAAG
Esp3I CGTCTC(1/5) BsmBI R0580 CGTCTC(1/5) BsmBI
EspI x GC/TNAGC BlpI R0585 GC/TNAGC BlpI, Bpu1102I, Bsp1720I
FaeI CATG/ CviAII^ R0640 C/ATG CviAII^, FatI^^, Hin1II, Hsp92II, NlaIII
FatI^^ R0650 /CATG
NlaIII R0125 CATG/
FaiI YA/TR
FalI (8/13)AAGNNNNNCTT(13/8)
FaqI GGGAC(10/14) BsmFI R0572 GGGAC(10/14) BslFI, BsmFI
FatI /CATG CviAII^ R0640 C/ATG CviAII^, FaeI^^, Hin1II^^, Hsp92II^^, NlaIII^^
FatI R0650 /CATG
NlaIII^^ R0125 CATG/
FauI CCCGC(4/6) FauI R0651 CCCGC(4/6)
FauNDI CA/TATG NdeI R0111 CA/TATG NdeI
FbaI T/GATCA BclI R0160 T/GATCA BclI, BclI-HF, Ksp22I
BclI-HF R3160 T/GATCA
FblI GT/MKAC AccI R0161 GT/MKAC AccI, XmiI
FinI x GGGAC BsmFI R0572 GGGAC(10/14) BslFI, BsmFI, FaqI
Fnu4HI GC/NGC Fnu4HI R0178 GC/NGC Fsp4HI, SatI
FnuDII x CG/CG BstUI R0518 CG/CG AccII, Bsh1236I, BspFNI, BstFNI, BstUI, MvnI
FokI GGATG(9/13) BtsCI^ R0647 GGATG(2/0) BseGI^, BstF5I^, BtsCI^
FokI R0109 GGATG(9/13)
FriOI GRGCY/C BanII R0119 GRGCY/C BanII, Eco24I, EcoT38I
FseI GGCCGG/CC FseI R0588 GGCCGG/CC RigI
Fsp4HI GC/NGC Fnu4HI R0178 GC/NGC Fnu4HI, SatI
FspAI RTGC/GCAY
FspBI C/TAG BfaI R0568 C/TAG BfaI, MaeI, SspMI, XspI
FspEI CC(12/16) FspEI R0662 CC(12/16)
FspI TGC/GCA FspI R0135 TGC/GCA Acc16I, NsbI
FtnUV x GAAACA
GauT27I x CGCGCAGG
Gba708II x ATGCAC
GdiII x CGGCCR(-5/-1)
GlaI GC/GC
GluI GC/NGC BisI, BlsI^, PkrI^
GsaI CCCAGC(-1/-5) BseYI^ R0635 CCCAGC(-5/-1) BseYI^, PspFI^
GsuI CTGGAG(16/14) BpmI R0565 CTGGAG(16/14) BpmI
HaeI x WGG/CCW
HaeII RGCGC/Y HaeII R0107 RGCGC/Y BfoI, BstH2I
HaeIII GG/CC HaeIII R0108 GG/CC BshFI, BsnI, BspANI, BsuRI
HaeIV x (7/13)GAYNNNNNRTC(14/9)
HapII C/CGG HpaII R0171 C/CGG BsiSI, HpaII, MspI
MspI R0106 C/CGG
HbaII x GCCCAG
DraIII-HF CACNNN/GTG DraIII-HF R3510 CACNNN/GTG AdeI, DraIII-HF
EagI-HF C/GGCCG EagI § R0505 C/GGCCG BseX3I, BstZI, EagI, EclXI, Eco52I
EagI-HF R3505 C/GGCCG
EcoRI-HF G/AATTC EcoRI § R0101 G/AATTC EcoRI
EcoRI-HF R3101 G/AATTC
EcoRV-HF GAT/ATC EcoRV § R0195 GAT/ATC Eco32I, EcoRV
EcoRV-HF R3195 GAT/ATC
ApoI-HF R/AATTY ApoI § R0566 R/AATTY AcsI, ApoI, XapI
ApoI-HF R3566 R/AATTY
AgeI-HF A/CCGGT AgeI § R0552 A/CCGGT AgeI, AsiGI, BshTI, CspAI, PinAI
AgeI-HF R3552 A/CCGGT
BmtI-HF G/CTAGC BmtI^ § R0658 GCTAG/C AsuNHI, BmtI^, BmtI-HF^, BspOI^, NheI
BmtI-HF^ R3658 GCTAG/C
NheI § R0131 G/CTAGC
NheI-HF R3131 G/CTAGC
BclI-HF T/GATCA BclI R0160 T/GATCA BclI, FbaI, Ksp22I
BclI-HF R3160 T/GATCA
BbsI-HF GAAGAC(2/6) BbsI § R0539 GAAGAC(2/6) BbsI, BpiI, BstV2I
BbsI-HF R3539 GAAGAC(2/6)
BamHI-HF G/GATCC BamHI § R0136 G/GATCC BamHI
BamHI-HF R3136 G/GATCC
BsiWI-HF C/GTACG BsiWI § R0553 C/GTACG BsiWI, Pfl23II, PspLI
BsiWI-HF R3553 C/GTACG
BsrGI-HF T/GTACA BsrGI § R0575 T/GTACA Bsp1407I, BsrGI, BstAUI
BsrGI-HF R3575 T/GTACA
BsaI-HF GGTCTC(1/5) BsaI § R0535 GGTCTC(1/5) BsaI, Bso31I, BspTNI, Eco31I
BsaI-HF R3535 GGTCTC(1/5)
BstEII-HF G/GTNACC BstEII § R0162 G/GTNACC BstEII, BstPI, Eco91I, EcoO65I, PspEI
BstEII-HF R3162 G/GTNACC
HgaI GACGC(5/10) HgaI R0154 GACGC(5/10) CseI
HgiAI x GWGCW/C BsiHKAI R0570 GWGCW/C Alw21I, Bbv12I, BsiHKAI
HgiCI x G/GYRCC BanI R0118 G/GYRCC AccB1I, BanI, BshNI, BspT107I
HgiEII x ACCNNNNNNGGT
HgiJII x GRGCY/C BanII R0119 GRGCY/C BanII, Eco24I, EcoT38I, FriOI
HhaI GCG/C HhaI R0139 GCG/C AspLEI, BstHHI, CfoI, Hin6I^, HinP1I^, HspAI^
HinP1I^ R0124 G/CGC
Hin1I GR/CGYC BsaHI R0556 GR/CGYC AcyI, BsaHI, BssNI, BstACI, Hsp92I
Hin1II CATG/ CviAII^ R0640 C/ATG CviAII^, FaeI, FatI^^, Hsp92II, NlaIII
FatI^^ R0650 /CATG
NlaIII R0125 CATG/
Hin4I x (8/13)GAYNNNNNVTC(13/8)
Hin4II x CCTTC(6/5) HpyAV R0621 CCTTC(6/5) HpyAV
Hin6I G/CGC HhaI^ R0139 GCG/C AspLEI^, BstHHI^, CfoI^, HhaI^, HinP1I, HspAI
HinP1I R0124 G/CGC
HincII GTY/RAC HincII R0103 GTY/RAC HindII
HindII GTY/RAC HincII R0103 GTY/RAC HincII
HindIII A/AGCTT HindIII § R0104 A/AGCTT HindIII-HF
HindIII-HF R3104 A/AGCTT
HindIII-HF A/AGCTT HindIII § R0104 A/AGCTT HindIII
HindIII-HF R3104 A/AGCTT
HinfI G/ANTC HinfI R0155 G/ANTC
HinP1I G/CGC HhaI^ R0139 GCG/C AspLEI^, BstHHI^, CfoI^, HhaI^, Hin6I, HspAI
HinP1I R0124 G/CGC
HpaI GTT/AAC HpaI R0105 GTT/AAC KspAI
HpaII C/CGG HpaII R0171 C/CGG BsiSI, HapII, MspI
MspI R0106 C/CGG
HphI GGTGA(8/7) HphI R0158 GGTGA(8/7) AsuHPI
Hpy166II GTN/NAC Hpy166II R0616 GTN/NAC Hpy8I
Hpy178III x TC/NNGA Hpy188III R0622 TC/NNGA Hpy188III
Hpy188I TCN/GA Hpy188I R0617 TCN/GA
Hpy188III TC/NNGA Hpy188III R0622 TC/NNGA
Hpy8I GTN/NAC Hpy166II R0616 GTN/NAC Hpy166II
Hpy99I CGWCG/ Hpy99I R0615 CGWCG/
Hpy99XIII x GCCTA
Hpy99XIV x GGWTAA
Hpy99XIV-mut1 x GGWCNA
Hpy99XXII x TCANNNNNNTRG
HpyAV CCTTC(6/5) HpyAV R0621 CCTTC(6/5)
HpyAXIV x GCGTA
HpyAXVI-mut1 x CRTTAA
HpyAXVI-mut2 x CRTCNA
HpyCH4III ACN/GT HpyCH4III R0618 ACN/GT Bst4CI, TaaI
HpyCH4IV A/CGT HpyCH4IV R0619 A/CGT HpySE526I, MaeII, TaiI^
HpyCH4V TG/CA HpyCH4V R0620 TG/CA
HpyF10VI GCNNNNN/NNGC MwoI R0573 GCNNNNN/NNGC BstMWI, MwoI
HpyF3I C/TNAG DdeI R0175 C/TNAG BstDEI, DdeI
HpySE526I A/CGT HpyCH4IV R0619 A/CGT HpyCH4IV, MaeII, TaiI^
HpyUM032XIII x CYANNNNNNNTRG
HpyUM032XIII-mut1 x CYANNNNNNNTTC
HpyUM032XIV x GAAAG
HpyUM037X x GTGGNAG, TNGGNAG
Hsp92I GR/CGYC BsaHI R0556 GR/CGYC AcyI, BsaHI, BssNI, BstACI, Hin1I
Hsp92II CATG/ CviAII^ R0640 C/ATG CviAII^, FaeI, FatI^^, Hin1II, NlaIII
FatI^^ R0650 /CATG
NlaIII R0125 CATG/
HspAI G/CGC HhaI^ R0139 GCG/C AspLEI^, BstHHI^, CfoI^, HhaI^, Hin6I, HinP1I
HinP1I R0124 G/CGC
Jma19592I x GTATNAC
Jma19592II x GRGCRAC
Jsp2502II x GRNGAAT
KasI G/GCGCC KasI R0544 G/GCGCC DinI^, EgeI^, EheI^, Mly113I^^, NarI^^, PluTI^^^, SfoI^, SspDI
NarI^^ R0191 GG/CGCC
PluTI^^^ R0713 GGCGC/C
SfoI^ R0606 GGC/GCC
KflI GG/GWCCC
Kor51II x RTCGAG
Kpn2I T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI
KpnI GGTAC/C Acc65I^ R0599 G/GTACC Acc65I^, Asp718I^, KpnI-HF
KpnI § R0142 GGTAC/C
KpnI-HF R3142 GGTAC/C
KpnI-HF GGTAC/C Acc65I^ R0599 G/GTACC Acc65I^, Asp718I^, KpnI
KpnI § R0142 GGTAC/C
KpnI-HF R3142 GGTAC/C
KpnNIH30III x GTTCNAC
KroI G/CCGGC
Ksp22I T/GATCA BclI R0160 T/GATCA BclI, BclI-HF, FbaI
BclI-HF R3160 T/GATCA
Ksp632I x CTCTTC(1/4) EarI R0528 CTCTTC(1/4) Bst6I, Eam1104I, EarI
KspAI GTT/AAC HpaI R0105 GTT/AAC HpaI
KspI CCGC/GG SacII R0157 CCGC/GG Cfr42I, SacII, Sfr303I, SgrBI
Kzo9I /GATC BfuCI R0636 /GATC BfuCI, Bsp143I, BssMI, BstKTI^, BstMBI, DpnII, MboI, NdeII, Sau3AI
DpnII R0543 /GATC
MboI R0147 /GATC
Sau3AI R0169 /GATC
Lba2029III x CYAAANG
LguI GCTCTTC(1/4) BspQI R0712 GCTCTTC(1/4) BspQI, PciSI, SapI
SapI R0569 GCTCTTC(1/4)
LmnI GCTCC(1/-1)
Lmo370I x AGCGCCG
Lmo911II x TAGRAG
LpnPI CCDG(10/14) LpnPI R0663 CCDG(10/14)
Lsp1109I GCAGC(8/12) BbvI R0173 GCAGC(8/12) BbvI, BseXI, BstV1I
Lsp48III x AGCACC
Lsp6406VI x CRAGCAC
LweI GCATC(5/9) SfaNI R0172 GCATC(5/9) BmsI, SfaNI
MabI A/CCWGGT SexAI R0605 A/CCWGGT CsiI, SexAI
MaeI C/TAG BfaI R0568 C/TAG BfaI, FspBI, SspMI, XspI
MaeII A/CGT HpyCH4IV R0619 A/CGT HpyCH4IV, HpySE526I, TaiI^
MaeIII /GTNAC
MalI GA/TC DpnI R0176 GA/TC DpnI
MaqI x CRTTGAC(21/19)
MauBI CG/CGCGCG
Mba11I x AGGCGA
MbiI CCGCTC(-3/-3) BsrBI R0102 CCGCTC(-3/-3) AccBSI, BsrBI
MboI /GATC BfuCI R0636 /GATC BfuCI, Bsp143I, BssMI, BstKTI^, BstMBI, DpnII, Kzo9I, NdeII, Sau3AI
DpnII R0543 /GATC
MboI R0147 /GATC
Sau3AI R0169 /GATC
MboII GAAGA(8/7) MboII R0148 GAAGA(8/7)
Mcr10I x GAAGNNNNNCTC
McrI x CGRY/CG BsiEI R0554 CGRY/CG Bsh1285I, BsiEI, BstMCI
MfeI C/AATTG MfeI § R0589 C/AATTG MfeI-HF, MunI
MfeI-HF R3589 C/AATTG
MfeI-HF C/AATTG MfeI § R0589 C/AATTG MfeI, MunI
MfeI-HF R3589 C/AATTG
MflI R/GATCY BstYI R0523 R/GATCY BstX2I, BstYI, PsuI
MhlI GDGCH/C Bsp1286I R0120 GDGCH/C Bsp1286I, SduI
MjaIV x GTNNAC Hpy166II R0616 GTN/NAC Hpy8I, Hpy166II
MkaDII x GAGAYGT
MlsI TGG/CCA MscI R0534 TGG/CCA BalI, MluNI, Mox20I, MscI, Msp20I
MluCI /AATT MluCI R0538 /AATT Sse9I, TasI
MluI A/CGCGT MluI § R0198 A/CGCGT MluI-HF
MluI-HF R3198 A/CGCGT
MluI-HF A/CGCGT MluI § R0198 A/CGCGT MluI
MluI-HF R3198 A/CGCGT
MluNI TGG/CCA MscI R0534 TGG/CCA BalI, MlsI, Mox20I, MscI, Msp20I
Mly113I GG/CGCC KasI^ R0544 G/GCGCC DinI^^, EgeI^^, EheI^^, KasI^, NarI, PluTI^^^, SfoI^^, SspDI^
NarI R0191 GG/CGCC
PluTI^^^ R0713 GGCGC/C
SfoI^^ R0606 GGC/GCC
MlyI GAGTC(5/5) MlyI R0610 GAGTC(5/5) PleI^, PpsI^, SchI
PleI^ R0515 GAGTC(4/5)
MmeI TCCRAC(20/18) MmeI R0637 TCCRAC(20/18)
MnlI CCTC(7/6) MnlI R0163 CCTC(7/6)
Mox20I TGG/CCA MscI R0534 TGG/CCA BalI, MlsI, MluNI, MscI, Msp20I
Mph1103I ATGCA/T NsiI § R0127 ATGCA/T EcoT22I, NsiI, NsiI-HF, Zsp2I
NsiI-HF R3127 ATGCA/T
MreI CG/CCGGCG
MroI T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I
MroNI G/CCGGC NaeI^ R0190 GCC/GGC NaeI^, NgoMIV, PdiI^
NgoMIV R0564 G/CCGGC
MroXI GAANN/NNTTC XmnI R0194 GAANN/NNTTC Asp700I, PdmI, XmnI
MscI TGG/CCA MscI R0534 TGG/CCA BalI, MlsI, MluNI, Mox20I, Msp20I
MseI T/TAA MseI R0525 T/TAA SaqAI, Tru1I, Tru9I
MslI CAYNN/NNRTG MslI R0571 CAYNN/NNRTG RseI, SmiMI
Msp20I TGG/CCA MscI R0534 TGG/CCA BalI, MlsI, MluNI, Mox20I, MscI
MspA1I CMG/CKG MspA1I R0577 CMG/CKG
MspCI C/TTAAG AflII R0520 C/TTAAG AflII, BfrI, BspTI, BstAFI, Vha464I
MspI C/CGG HpaII R0171 C/CGG BsiSI, HapII, HpaII
MspI R0106 C/CGG
MspI7II x ACGRAG
MspJI CNNR(9/13) MspJI R0661 CNNR(9/13)
MspR9I CC/NGG ScrFI R0110 CC/NGG Bme1390I, BmrFI, BstSCI^, ScrFI, StyD4I^
StyD4I^ R0638 /CCNGG
MssI GTTT/AAAC PmeI R0560 GTTT/AAAC PmeI
MstI x TGC/GCA FspI R0135 TGC/GCA Acc16I, FspI, NsbI
MteI GCGC/NGCGC
MtuHN878II x CACGCAG
MunI C/AATTG MfeI § R0589 C/AATTG MfeI, MfeI-HF
MfeI-HF R3589 C/AATTG
Mva1269I GAATGC(1/-1) BsmI R0134 GAATGC(1/-1) BsmI, PctI
MvaI CC/WGG BstNI R0168 CC/WGG AjnI^, BciT130I, BseBI, BstNI, Bst2UI, EcoRII^, Psp6I^, PspGI^
PspGI^ R0611 /CCWGG
MvnI CG/CG BstUI R0518 CG/CG AccII, Bsh1236I, BspFNI, BstFNI, BstUI
MwoI GCNNNNN/NNGC MwoI R0573 GCNNNNN/NNGC BstMWI, HpyF10VI
NaeI GCC/GGC NaeI R0190 GCC/GGC MroNI^, NgoMIV^, PdiI
NgoMIV^ R0564 G/CCGGC
Nal45188II x ACCAGC
NarI GG/CGCC KasI^ R0544 G/GCGCC DinI^^, EgeI^^, EheI^^, KasI^, Mly113I, PluTI^^^, SfoI^^, SspDI^
NarI R0191 GG/CGCC
PluTI^^^ R0713 GGCGC/C
SfoI^^ R0606 GGC/GCC
Nbr128II x ACCGAC
NciI CC/SGG NciI R0196 CC/SGG AsuC2I, BcnI, BpuMI
NcoI C/CATGG NcoI § R0193 C/CATGG Bsp19I, NcoI-HF
NcoI-HF R3193 C/CATGG
NcoI-HF C/CATGG NcoI § R0193 C/CATGG Bsp19I, NcoI
NcoI-HF R3193 C/CATGG
NdeI CA/TATG NdeI R0111 CA/TATG FauNDI
NdeII /GATC BfuCI R0636 /GATC BfuCI, Bsp143I, BssMI, BstKTI^, BstMBI, DpnII, Kzo9I, MboI, Sau3AI
DpnII R0543 /GATC
MboI R0147 /GATC
Sau3AI R0169 /GATC
NgoAVII x GCCGC(7/7)
NgoAVIII x (12/14)GACNNNNNTGA(13/11)
NgoMIV G/CCGGC NaeI^ R0190 GCC/GGC MroNI, NaeI^, PdiI^
NgoMIV R0564 G/CCGGC
NhaXI x CAAGRAG
NheI G/CTAGC BmtI^ § R0658 GCTAG/C AsuNHI, BmtI^, BmtI-HF^, BspOI^, NheI-HF
BmtI-HF^ R3658 GCTAG/C
NheI § R0131 G/CTAGC
NheI-HF R3131 G/CTAGC
NheI-HF G/CTAGC BmtI^ § R0658 GCTAG/C AsuNHI, BmtI^, BmtI-HF^, BspOI^, NheI
BmtI-HF^ R3658 GCTAG/C
NheI § R0131 G/CTAGC
NheI-HF R3131 G/CTAGC
NhoI x GCWGC
NlaCI x CATCAC(19/17)
NlaIII CATG/ CviAII^ R0640 C/ATG CviAII^, FaeI, FatI^^, Hin1II, Hsp92II
FatI^^ R0650 /CATG
NlaIII R0125 CATG/
NlaIV GGN/NCC NlaIV R0126 GGN/NCC BmiI, BspLI, PspN4I
NmeAIII GCCGAG(21/19) NmeAIII R0711 GCCGAG(21/19)
NmuCI /GTSAC Tsp45I R0583 /GTSAC TseFI, Tsp45I
NotI GC/GGCCGC NotI § R0189 GC/GGCCGC CciNI, NotI-HF
NotI-HF R3189 GC/GGCCGC
NotI-HF GC/GGCCGC NotI § R0189 GC/GGCCGC CciNI, NotI
NotI-HF R3189 GC/GGCCGC
NpeUS61II x GATCGAC
NruI-HF TCG/CGA NruI § R0192 TCG/CGA Bsp68I, BtuMI, NruI, RruI
NruI-HF R3192 TCG/CGA
NruI TCG/CGA NruI § R0192 TCG/CGA Bsp68I, BtuMI, NruI-HF, RruI
NruI-HF R3192 TCG/CGA
NsbI TGC/GCA FspI R0135 TGC/GCA Acc16I, FspI
NsiI ATGCA/T NsiI § R0127 ATGCA/T EcoT22I, Mph1103I, NsiI-HF, Zsp2I
NsiI-HF R3127 ATGCA/T
NsiI-HF ATGCA/T NsiI § R0127 ATGCA/T EcoT22I, Mph1103I, NsiI, Zsp2I
NsiI-HF R3127 ATGCA/T
NspBII x CMG/CKG MspA1I R0577 CMG/CKG MspA1I
NspI RCATG/Y NspI R0602 RCATG/Y BstNSI, XceI
NspV TT/CGAA BstBI R0519 TT/CGAA AsuII, Bpu14I, Bsp119I, BspT104I, BstBI, SfuI
OliI CACNN/NNGTG AleI R0634 CACNN/NNGTG AleI
OspHL35III x YAGGAG
Pac19842II x CCTTGA
PacI TTAAT/TAA PacI R0547 TTAAT/TAA
PacIII x GTAATC
PaeI GCATG/C SphI § R0182 GCATG/C SphI, SphI-HF
SphI-HF R3182 GCATG/C
PaeR7I C/TCGAG PaeR7I R0177 C/TCGAG Sfr274I, SlaI, XhoI
XhoI R0146 C/TCGAG
PagI T/CATGA BspHI R0517 T/CATGA BspHI, CciI
Pal408I x CCRTGAG
PalAI GG/CGCGCC AscI R0558 GG/CGCGCC AscI, SgsI
PasI CC/CWGGG
PauI G/CGCGC BssHII R0199 G/CGCGC BsePI, BssHII, PteI
PcaII x GACGAG
PceI AGG/CCT StuI R0187 AGG/CCT Eco147I, SseBI, StuI
PciI A/CATGT PciI R0655 A/CATGT PscI
PciSI GCTCTTC(1/4) BspQI R0712 GCTCTTC(1/4) BspQI, LguI, SapI
SapI R0569 GCTCTTC(1/4)
PcsI WCGNNNN/NNNCGW
PctI GAATGC(1/-1) BsmI R0134 GAATGC(1/-1) BsmI, Mva1269I
Pdi8503III x CCGGNAG
PdiI GCC/GGC NaeI R0190 GCC/GGC MroNI^, NaeI, NgoMIV^
NgoMIV^ R0564 G/CCGGC
PdmI GAANN/NNTTC XmnI R0194 GAANN/NNTTC Asp700I, MroXI, XmnI
Pdu1735I x CACCAC
PenI x GCAGT
PfeI G/AWTC TfiI R0546 G/AWTC TfiI
Pfl1108I x TCGTAG
Pfl23II C/GTACG BsiWI § R0553 C/GTACG BsiWI, BsiWI-HF, PspLI
BsiWI-HF R3553 C/GTACG
Pfl8569I x GCN/NGC
PflFI GACN/NNGTC PflFI R0595 GACN/NNGTC PsyI, Tth111I
Tth111I R0185 GACN/NNGTC
PflMI CCANNNN/NTGG PflMI R0509 CCANNNN/NTGG AccB7I, Van91I
PfoI T/CCNGGA
PfrUF1II x CTTCNAC
PinAI A/CCGGT AgeI § R0552 A/CCGGT AgeI, AgeI-HF, AsiGI, BshTI, CspAI
AgeI-HF R3552 A/CCGGT
PkrI GCN/GC BisI^, BlsI, GluI^
Ple19I CGAT/CG PvuI § R0150 CGAT/CG PvuI, PvuI-HF
PvuI-HF R3150 CGAT/CG
PleI GAGTC(4/5) MlyI^ R0610 GAGTC(5/5) MlyI^, PpsI, SchI^
PleI R0515 GAGTC(4/5)
PliMI x CGCCGAC
PluTI GGCGC/C KasI^ R0544 G/GCGCC DinI^^, EgeI^^, EheI^^, KasI^, Mly113I^^^, NarI^^^, SfoI^^, SspDI^
NarI^^^ R0191 GG/CGCC
PluTI R0713 GGCGC/C
SfoI^^ R0606 GGC/GCC
PmaCI CAC/GTG PmlI R0532 CAC/GTG AcvI, BbrPI, Eco72I, PmlI, PspCI
PmeI GTTT/AAAC PmeI R0560 GTTT/AAAC MssI
PmlI CAC/GTG PmlI R0532 CAC/GTG AcvI, BbrPI, Eco72I, PmaCI, PspCI
PpiI x (7/12)GAACNNNNNCTC(13/8)
PpsI GAGTC(4/5) MlyI^ R0610 GAGTC(5/5) MlyI^, PleI, SchI^
PleI R0515 GAGTC(4/5)
Ppu21I YAC/GTR BsaAI R0531 YAC/GTR BsaAI, BstBAI
PpuMI RG/GWCCY PpuMI R0506 RG/GWCCY Psp5II, PspPPI
PscI A/CATGT PciI R0655 A/CATGT PciI
Pse18267I x RCCGAAG
PshAI GACNN/NNGTC PshAI R0593 GACNN/NNGTC BoxI, BstPAI
PshBI AT/TAAT AseI R0526 AT/TAAT AseI, VspI
PsiI TTA/TAA PsiI R0657 TTA/TAA AanI
Psp0357II x GCGAAG
Psp124BI GAGCT/C Eco53kI^ R0116 GAG/CTC Ecl136II^, EcoICRI^, Eco53kI^, SacI, SacI-HF, SstI
SacI § R0156 GAGCT/C
SacI-HF R3156 GAGCT/C
Psp1406I AA/CGTT AclI R0598 AA/CGTT AclI
Psp5II RG/GWCCY PpuMI R0506 RG/GWCCY PpuMI, PspPPI
Psp6I /CCWGG BstNI^ R0168 CC/WGG AjnI, BciT130I^, BseBI^, BstNI^, Bst2UI^, EcoRII, MvaI^, PspGI
PspGI R0611 /CCWGG
PspCI CAC/GTG PmlI R0532 CAC/GTG AcvI, BbrPI, Eco72I, PmaCI, PmlI
PspEI G/GTNACC BstEII § R0162 G/GTNACC BstEII, BstEII-HF, BstPI, Eco91I, EcoO65I
BstEII-HF R3162 G/GTNACC
PspFI CCCAGC(-5/-1) BseYI R0635 CCCAGC(-5/-1) BseYI, GsaI^
PspGI /CCWGG BstNI^ R0168 CC/WGG AjnI, BciT130I^, BseBI^, BstNI^, Bst2UI^, EcoRII, MvaI^, Psp6I
PspGI R0611 /CCWGG
PspLI C/GTACG BsiWI § R0553 C/GTACG BsiWI, BsiWI-HF, Pfl23II
BsiWI-HF R3553 C/GTACG
PspN4I GGN/NCC NlaIV R0126 GGN/NCC BmiI, BspLI, NlaIV
PspOMI G/GGCCC ApaI^ R0114 GGGCC/C ApaI^, Bsp120I
PspOMI R0653 G/GGCCC
PspOMII x CGCCCAR(20/18)
PspPI G/GNCC Sau96I R0165 G/GNCC AspS9I, BmgT120I, Cfr13I, Sau96I
PspPPI RG/GWCCY PpuMI R0506 RG/GWCCY PpuMI, Psp5II
PspPRI x CCYCAG(15/13)
PspXI VC/TCGAGB PspXI R0656 VC/TCGAGB
PsrI (7/12)GAACNNNNNNTAC(12/7)
Pst14472I x CNYACAC
Pst273I x GATCGAG
PstI CTGCA/G PstI § R0140 CTGCA/G BspMAI, PstI-HF
PstI-HF R3140 CTGCA/G
PstI-HF CTGCA/G PstI § R0140 CTGCA/G BspMAI, PstI
PstI-HF R3140 CTGCA/G
PstNI CAGNNN/CTG AlwNI R0514 CAGNNN/CTG AlwNI, CaiI
PsuGI x BBCGD
PsuI R/GATCY BstYI R0523 R/GATCY BstX2I, BstYI, MflI
PsyI GACN/NNGTC PflFI R0595 GACN/NNGTC PflFI, Tth111I
Tth111I R0185 GACN/NNGTC
PteI G/CGCGC BssHII R0199 G/CGCGC BsePI, BssHII, PauI
PvuI CGAT/CG PvuI § R0150 CGAT/CG Ple19I, PvuI-HF
PvuI-HF R3150 CGAT/CG
PvuI-HF CGAT/CG PvuI § R0150 CGAT/CG Ple19I, PvuI
PvuI-HF R3150 CGAT/CG
PvuII CAG/CTG PvuII § R0151 CAG/CTG PvuII-HF
PvuII-HF R3151 CAG/CTG
PvuII-HF CAG/CTG PvuII § R0151 CAG/CTG PvuII
PvuII-HF R3151 CAG/CTG
Rba2021I x CACGAGH
RceI x CATCGAC(20/18)
RdeGBI x CCGCAG
RdeGBII x ACCCAG(20/18)
RdeGBIII x (9/11)TGRYCA(11/9)
RflFIII x CGCCAG
RgaI GCGAT/CGC AsiSI R0630 GCGAT/CGC AsiSI, SfaAI, SgfI
RigI GGCCGG/CC FseI R0588 GGCCGG/CC FseI
RlaI x VCW
RlaII x ACACAG(20/18)
RleAI x CCCACA(12/9)
RpaB5I x CGRGGAC(20/18)
RpaBI x CCCGCAG(20/18)
RpaI x GTYGGAG(11/9)
RpaTI x GRTGGAG
RruI TCG/CGA NruI § R0192 TCG/CGA Bsp68I, BtuMI, NruI, NruI-HF
NruI-HF R3192 TCG/CGA
RsaI GT/AC CviQI^ R0639 G/TAC AfaI, Csp6I^, CviQI^, RsaNI^
RsaI R0167 GT/AC
RsaNI G/TAC CviQI R0639 G/TAC AfaI^, Csp6I, CviQI, RsaI^
RsaI^ R0167 GT/AC
RseI CAYNN/NNRTG MslI R0571 CAYNN/NNRTG MslI, SmiMI
Rsp008IV x ACGCAG
Rsp008V x GCCCAT
RspPBTS2III x CTTCGAG
Rsr2I CG/GWCCG RsrII R0501 CG/GWCCG CpoI, CspI, RsrII
RsrII CG/GWCCG RsrII R0501 CG/GWCCG CpoI, CspI, Rsr2I
SacI GAGCT/C Eco53kI^ R0116 GAG/CTC Ecl136II^, EcoICRI^, Eco53kI^, Psp124BI, SacI-HF, SstI
SacI § R0156 GAGCT/C
SacI-HF R3156 GAGCT/C
SacI-HF GAGCT/C Eco53kI^ R0116 GAG/CTC Ecl136II^, EcoICRI^, Eco53kI^, Psp124BI, SacI, SstI
SacI § R0156 GAGCT/C
SacI-HF R3156 GAGCT/C
SacII CCGC/GG SacII R0157 CCGC/GG Cfr42I, KspI, Sfr303I, SgrBI
Saf8902III x CAATNAG
SalI-HF G/TCGAC SalI § R0138 G/TCGAC SalI
SalI-HF R3138 G/TCGAC
SalI G/TCGAC SalI § R0138 G/TCGAC SalI-HF
SalI-HF R3138 G/TCGAC
SanDI x GG/GWCCC KflI
SapI GCTCTTC(1/4) BspQI R0712 GCTCTTC(1/4) BspQI, LguI, PciSI
SapI R0569 GCTCTTC(1/4)
SaqAI T/TAA MseI R0525 T/TAA MseI, Tru1I, Tru9I
SatI GC/NGC Fnu4HI R0178 GC/NGC Fnu4HI, Fsp4HI
Sau3AI /GATC BfuCI R0636 /GATC BfuCI, Bsp143I, BssMI, BstKTI^, BstMBI, DpnII, Kzo9I, MboI, NdeII
DpnII R0543 /GATC
MboI R0147 /GATC
Sau3AI R0169 /GATC
Sau96I G/GNCC Sau96I R0165 G/GNCC AspS9I, BmgT120I, Cfr13I, PspPI
SauI x CC/TNAGG Bsu36I R0524 CC/TNAGG AxyI, Bse21I, Bsu36I, Eco81I
SbfI-HF CCTGCA/GG SbfI § R0642 CCTGCA/GG SbfI, SdaI, Sse8387I
SbfI-HF R3642 CCTGCA/GG
SbfI CCTGCA/GG SbfI § R0642 CCTGCA/GG SbfI-HF, SdaI, Sse8387I
SbfI-HF R3642 CCTGCA/GG
Sbo46I x TGAAC
ScaI-HF AGT/ACT ScaI-HF R3122 AGT/ACT BmcAI, ScaI-HF, ZrmI
SchI GAGTC(5/5) MlyI R0610 GAGTC(5/5) MlyI, PleI^, PpsI^
PleI^ R0515 GAGTC(4/5)
ScoDS2II x GCTAAT
ScrFI CC/NGG ScrFI R0110 CC/NGG Bme1390I, BmrFI, BstSCI^, MspR9I, StyD4I^
StyD4I^ R0638 /CCNGG
SdaI CCTGCA/GG SbfI § R0642 CCTGCA/GG SbfI, SbfI-HF, Sse8387I
SbfI-HF R3642 CCTGCA/GG
SdeAI x CAGRAG(21/19)
SdeOSI x (11/13)GACNNNNRTGA(12/10)
SduI GDGCH/C Bsp1286I R0120 GDGCH/C Bsp1286I, MhlI
SecI x C/CNNGG BsaJI R0536 C/CNNGG BsaJI, BseDI, BssECI
SetI ASST/
SexAI A/CCWGGT SexAI R0605 A/CCWGGT CsiI, MabI
SfaAI GCGAT/CGC AsiSI R0630 GCGAT/CGC AsiSI, RgaI, SgfI
SfaNI GCATC(5/9) SfaNI R0172 GCATC(5/9) BmsI, LweI
SfcI C/TRYAG SfcI R0561 C/TRYAG BfmI, BstSFI
SfeI x C/TRYAG SfcI R0561 C/TRYAG BfmI, BstSFI, SfcI
SfiI GGCCNNNN/NGGCC SfiI R0123 GGCCNNNN/NGGCC
SfoI GGC/GCC KasI^ R0544 G/GCGCC DinI, EgeI, EheI, KasI^, Mly113I^^, NarI^^, PluTI^^^, SspDI^
NarI^^ R0191 GG/CGCC
PluTI^^^ R0713 GGCGC/C
SfoI R0606 GGC/GCC
Sfr274I C/TCGAG PaeR7I R0177 C/TCGAG PaeR7I, SlaI, XhoI
XhoI R0146 C/TCGAG
Sfr303I CCGC/GG SacII R0157 CCGC/GG Cfr42I, KspI, SacII, SgrBI
SfuI TT/CGAA BstBI R0519 TT/CGAA AsuII, Bpu14I, Bsp119I, BspT104I, BstBI, NspV
SgeI CNNGNNNNNNNNN/
SgfI GCGAT/CGC AsiSI R0630 GCGAT/CGC AsiSI, RgaI, SfaAI
SgrAI CR/CCGGYG SgrAI R0603 CR/CCGGYG
SgrBI CCGC/GG SacII R0157 CCGC/GG Cfr42I, KspI, SacII, Sfr303I
SgrDI CG/TCGACG
SgrTI x CCDS(10/14)
SgsI GG/CGCGCC AscI R0558 GG/CGCGCC AscI, PalAI
SimI x GGGTC(-3/0)
SinI G/GWCC AvaII R0153 G/GWCC AvaII, Bme18I, Eco47I, VpaK11BI
SlaI C/TCGAG PaeR7I R0177 C/TCGAG PaeR7I, Sfr274I, XhoI
XhoI R0146 C/TCGAG
SmaI CCC/GGG SmaI R0141 CCC/GGG Cfr9I^, TspMI^, XmaI^
TspMI^ R0709 C/CCGGG
XmaI^ R0180 C/CCGGG
SmiI ATTT/AAAT SwaI R0604 ATTT/AAAT SwaI
SmiMI CAYNN/NNRTG MslI R0571 CAYNN/NNRTG MslI, RseI
SmlI C/TYRAG SmlI R0597 C/TYRAG SmoI
SmoI C/TYRAG SmlI R0597 C/TYRAG SmlI
SnaBI TAC/GTA SnaBI R0130 TAC/GTA BstSNI, Eco105I
SnaI x GTATAC BstZ17I R3594 GTA/TAC BssNAI, Bst1107I, BstZ17I-HF
BstZ17I-HF R3594 GTA/TAC
Sno506I x GGCCGAG
SpeI A/CTAGT SpeI § R0133 A/CTAGT AhlI, BcuI, SpeI-HF
SpeI-HF R3133 A/CTAGT
SpeI-HF A/CTAGT SpeI § R0133 A/CTAGT AhlI, BcuI, SpeI
SpeI-HF R3133 A/CTAGT
SphI GCATG/C SphI § R0182 GCATG/C PaeI, SphI-HF
SphI-HF R3182 GCATG/C
SphI-HF GCATG/C SphI § R0182 GCATG/C PaeI, SphI
SphI-HF R3182 GCATG/C
SplI x C/GTACG BsiWI § R0553 C/GTACG BsiWI, BsiWI-HF, Pfl23II, PspLI
BsiWI-HF R3553 C/GTACG
SpnRII x TCGAG
SpoDI x GCGGRAG
SrfI GCCC/GGGC SrfI R0629 GCCC/GGGC
Sse232I x CG/CCGGCG MreI
Sse8387I CCTGCA/GG SbfI § R0642 CCTGCA/GG SbfI, SbfI-HF, SdaI
SbfI-HF R3642 CCTGCA/GG
Sse8647I x AG/GWCCT
Sse9I /AATT MluCI R0538 /AATT MluCI, TasI
SseBI AGG/CCT StuI R0187 AGG/CCT Eco147I, PceI, StuI
SsiI CCGC(-3/-1) AciI R0551 CCGC(-3/-1) AciI, BspACI
Ssp6803IV x GAAGGC
Ssp714II x CGCAGCG
SspDI G/GCGCC KasI R0544 G/GCGCC DinI^, EgeI^, EheI^, KasI, Mly113I^^, NarI^^, PluTI^^^, SfoI^
NarI^^ R0191 GG/CGCC
PluTI^^^ R0713 GGCGC/C
SfoI^ R0606 GGC/GCC
SspI-HF AAT/ATT SspI-HF R3132 AAT/ATT SspI-HF
SspMI C/TAG BfaI R0568 C/TAG BfaI, FspBI, MaeI, XspI
SstE37I x CGAAGAC(20/18)
SstI GAGCT/C Eco53kI^ R0116 GAG/CTC Ecl136II^, EcoICRI^, Eco53kI^, Psp124BI, SacI, SacI-HF
SacI § R0156 GAGCT/C
SacI-HF R3156 GAGCT/C
Sth132I x CCCG(4/8)
Sth20745III x GGACGAC
StuI AGG/CCT StuI R0187 AGG/CCT Eco147I, PceI, SseBI
Sty13348III x GATCAG
StyD4I /CCNGG ScrFI^ R0110 CC/NGG Bme1390I^, BmrFI^, BstSCI, MspR9I^, ScrFI^
StyD4I R0638 /CCNGG
StyI C/CWWGG StyI § R0500 C/CWWGG BssT1I, Eco130I, EcoT14I, ErhI, StyI-HF
StyI-HF R3500 C/CWWGG
StyI-HF C/CWWGG StyI § R0500 C/CWWGG BssT1I, Eco130I, EcoT14I, ErhI, StyI
StyI-HF R3500 C/CWWGG
SwaI ATTT/AAAT SwaI R0604 ATTT/AAAT SmiI
TaaI ACN/GT HpyCH4III R0618 ACN/GT Bst4CI, HpyCH4III
TaiI ACGT/ HpyCH4IV^ R0619 A/CGT HpyCH4IV^, HpySE526I^, MaeII^
TaqI T/CGA TaqI R0149 T/CGA
TaqII GACCGA(11/9)
TaqIII x CACCCA(11/9)
TasI /AATT MluCI R0538 /AATT MluCI, Sse9I
TatI W/GTACW
TauI GCSG/C
TfiI G/AWTC TfiI R0546 G/AWTC PfeI
Tru1I T/TAA MseI R0525 T/TAA MseI, SaqAI, Tru9I
Tru9I T/TAA MseI R0525 T/TAA MseI, SaqAI, Tru1I
TscAI CASTGNN/ TspRI R0582 CASTGNN/ TspRI
TseFI /GTSAC Tsp45I R0583 /GTSAC NmuCI, Tsp45I
TseI G/CWGC ApeKI R0643 G/CWGC ApeKI
TseI R0591 G/CWGC
TsoI x TARCCA(11/9)
Tsp45I /GTSAC Tsp45I R0583 /GTSAC NmuCI, TseFI
Tsp4CI x ACN/GT HpyCH4III R0618 ACN/GT Bst4CI, HpyCH4III, TaaI
TspARh3I x GRACGAC
TspDTI ATGAA(11/9)
TspEI x /AATT MluCI R0538 /AATT MluCI, Sse9I, TasI
TspGWI ACGGA(11/9)
TspMI C/CCGGG SmaI^ R0141 CCC/GGG Cfr9I, SmaI^, XmaI
TspMI R0709 C/CCGGG
XmaI R0180 C/CCGGG
TspRI CASTGNN/ TspRI R0582 CASTGNN/ TscAI
TssI x GAGNNNCTC
TstI x (8/13)CACNNNNNNTCC(12/7)
TsuI x GCGAC
Tth111I GACN/NNGTC PflFI R0595 GACN/NNGTC PflFI, PsyI
Tth111I R0185 GACN/NNGTC
Tth111II x CAARCA(11/9)
UbaF11I x TCGTA
UbaF12I x CTACNNNGTC
UbaF13I x GAGNNNNNNCTGG
UbaF14I x CCANNNNNTCG
UbaF9I x TACNNNNNRTGT
UbaPI x CGAACG
Van91I CCANNNN/NTGG PflMI R0509 CCANNNN/NTGG AccB7I, PflMI
Vha464I C/TTAAG AflII R0520 C/TTAAG AflII, BfrI, BspTI, BstAFI, MspCI
VneI G/TGCAC ApaLI R0507 G/TGCAC Alw44I, ApaLI
VpaK11BI G/GWCC AvaII R0153 G/GWCC AvaII, Bme18I, Eco47I, SinI
VspI AT/TAAT AseI R0526 AT/TAAT AseI, PshBI
Vtu19109I x CACRAYC
WviI x CACRAG(21/19)
XagI CCTNN/NNNAGG EcoNI R0521 CCTNN/NNNAGG BstENI, EcoNI
XapI R/AATTY ApoI § R0566 R/AATTY AcsI, ApoI, ApoI-HF
ApoI-HF R3566 R/AATTY
XbaI T/CTAGA XbaI R0145 T/CTAGA
Xca85IV x TACGAG
XceI RCATG/Y NspI R0602 RCATG/Y BstNSI, NspI
XcmI CCANNNNN/NNNNTGG XcmI R0533 CCANNNNN/NNNNTGG
XhoI C/TCGAG PaeR7I R0177 C/TCGAG PaeR7I, Sfr274I, SlaI
XhoI R0146 C/TCGAG
XhoII x R/GATCY BstYI R0523 R/GATCY BstX2I, BstYI, MflI, PsuI
XmaI C/CCGGG SmaI^ R0141 CCC/GGG Cfr9I, SmaI^, TspMI
TspMI R0709 C/CCGGG
XmaI R0180 C/CCGGG
XmaIII x C/GGCCG EagI § R0505 C/GGCCG BseX3I, BstZI, EagI, EagI-HF, EclXI, Eco52I
EagI-HF R3505 C/GGCCG
XmaJI C/CTAGG AvrII R0174 C/CTAGG AspA2I, AvrII, BlnI
XmiI GT/MKAC AccI R0161 GT/MKAC AccI, FblI
XmnI GAANN/NNTTC XmnI R0194 GAANN/NNTTC Asp700I, MroXI, PdmI
XspI C/TAG BfaI R0568 C/TAG BfaI, FspBI, MaeI, SspMI
Yps3606I x CGGAAG
ZraI GAC/GTC AatII^ R0117 GACGT/C AatII^
ZraI R0659 GAC/GTC
ZrmI AGT/ACT ScaI R3122 AGT/ACT BmcAI, ScaI-HF
ScaI-HF R3122 AGT/ACT
Zsp2I ATGCA/T NsiI § R0127 ATGCA/T EcoT22I, Mph1103I, NsiI, NsiI-HF
NsiI-HF R3127 ATGCA/T

§ An HF version of this enzyme is available.
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