Discontinued Products (>3 years) and Their Alternatives

The following products have been discontinued for more than 3 years and they are no longer available. Some of them may have alternatives or replacements, please contact Technical Support if you have any questions or need information about them. For products that have been discontinued for less than three years, click here.

Catalog Number Product Name Discontinued Date Replacement Products
B0211 EcoRI Methyltransferase Reaction Buffer 1/1/2013
B0324 Crimson Taq Reaction Buffer Pack 1/5/2015
B0297 RNase H Reaction Buffer 1/5/2015
B0348 NEBNext® dsDNA Fragmentase® Reaction Buffer 1/9/2019
B0332 Hemo KlenTaq® Reaction Buffer Pack 1/5/2017
B0760 NEBuffer for Protein Tyrosine Phosphatases (PTP) 1/1/2013
B0322 B0322 LongAmp® Taq Reaction Buffer Pack 1/1/2013
B0253 M-MuLV Reverse Transcriptase Reaction Buffer 1/5/2017
B0250 Mung Bean Nuclease Reaction Buffer 1/1/2013
B3321 Luciferase Cell Lysis Buffer 8/2/2018
B0262 Lambda Exonuclease Reaction Buffer 1/5/2015
B0323 LongAmp® Taq Reaction Buffer Pack 1/5/2017
B9012 RNAPol Reaction Buffer 11/17/2014
B9023 OneTaq® GC Reaction Buffer Pack 1/5/2017
B9024 OneTaq® (Mg-free) Standard Reaction Buffer Pack 1/5/2015
B0205 E. coli DNA Ligase Reaction Buffer 1/1/2013
B0368 ProtoScript® II Reverse Transcriptase Reaction Buffer 1/5/2015
B8005 Diluent E 1/1/2013
B8004 Diluent D 1/1/2013
B8006 Diluent F 1/1/2013
B0298 Cre Recombinase Reaction Buffer 1/1/2013
B6000 PKA Reaction Buffer 1/1/2013
B9022 OneTaq® Standard Reaction Buffer Pack 1/5/2017
B0269 phi29 DNA Polymerase Reaction Buffer 1/5/2017
B0315 Terminal Transferase Reaction Buffer Pack 1/5/2015
B9025 OneTaq® (Mg-free) GC Reaction Buffer Pack 1/5/2015
B9001 Purified BSA 6/4/2013 B9000
B0213 Nuclease BAL-31 Reaction Buffer 1/1/2013
B0520 Detergent-free Phusion® HF Buffer Pack 1/5/2015
B0268 Endonuclease III (Nth) Reaction Buffer 1/1/2013
B0490 EpiMark® Hot Start Taq Reaction Buffer Pack 1/1/2013
B0719 Exoglycosidase Reaction Buffer Pack 1/5/2015
B8104 Endoproteinase AspN Reaction Buffer 1/1/2013
B9005 ThermoPol® II (Mg-free) Reaction Buffer Pack 7/8/2019
B0334 Therminator™ γ Reaction Buffer Pack 1/1/2013
B8101 Trypsin-ultra, Reaction Buffer 1/1/2013
B0280 UDG Reaction Buffer 1/5/2015
B9013 ThermoPol® DF (Detergent-free) Reaction Buffer Pack 1/1/2016
B0392 β-Agarase I Reaction Buffer 1/5/2015
B8100 GluC Reaction Buffer 1/1/2013
B7709 Red Loading Buffer Pack 11/13/2017 B7703
B6040 GSK-3 Reaction Buffer 1/1/2013
B6022 NEBuffer™ for Protein Kinases (PK) 1/2/2018
B0761 NEBuffer Pack for Protein MetalloPhosphatases (PMP) 1/5/2015
B0696 NEBuffer™ PI-SceI 1/1/2013
B0695 NEBuffer™ PI-SceI 1/1/2013
B7000 NEBuffer Set (1, 2, 3, 4 & BSA) 1/1/2013
B6030 CK1 Reaction Buffer 1/1/2013
B6010 CK2 Reaction Buffer 1/1/2013
B7011 CDP-Star® Dilution Buffer (25X) 1/1/2013
B0325 Crimson Taq (Mg-free) Reaction Buffer Pack 1/5/2015
B8000 Diluent Set (A, B and C) 1/1/2013
B0521 Detergent-free Phusion® GC Buffer Pack 1/5/2015
B0326 Crimson LongAmp™ Taq Reaction Buffer Pack 1/1/2013
B0694 NEBuffer™ I-SceI 1/1/2013
C3022 T7 Express Crystal Competent E. coli (High Efficiency) 1/2/2018
C3009 T7 Express High Efficiency Sampler 3/9/2015
C3016 T7 Express Iq Competent E. coli (High Efficiency) 3/31/2015 C3013
C1002 K. lactis YCT284 Competent Cells 1/5/2015
C1007 K. lactis Protease Deficient Competent Cell Sampler 9/15/2014
E6000 NEBNext® DNA Library Prep Reagent Set for Illumina® 6/15/2017 E7645
E6100 NEBNext® mRNA Library Prep Reagent Set for Illumina® 2/17/2017 E7760, E7770
E6090 NEBNext® Quick DNA Library Prep Master Mix Set for 454™ 1/19/2016
E6146 NEBNext® RNase III RNA Fragmentation Module 1/1/2017 E6150
E7450 NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1) 7/11/2014
E6071 NEBNext® Fill-in and ssDNA Isolation Module 1/1/2013
E2030 HiScribe™ T7 In Vitro Transcription Kit 1/13/2013 E2050
E8046 MBP5 Protein 1/5/2015
E1400 NEB® Essential Cloning Set 8/1/2014
E6200 NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® 6/15/2017 E7645
E6260 NEBNext® ChIP-Seq Library Prep Set for SOLiD™ 1/1/2013
E6040 NEBNext® DNA Library Prep Master Mix Set for Illumina 1/1/2020 E7645, E6050, E6056, E6053, M0543, E7103, E7805, E6177
E9300 ACP-Surface Starter Kit 1/1/2017
E3312 p19 miRNA Detection Kit 12/9/2015
E9120 SNAP-Surface® Starter Kit 9/14/2017
E4123 E. coli K12 CAG597 3/7/2018
E4141 E. coli K12 CJ236 3/7/2018
E4101 E. coli K12 ER1793 3/7/2018
E4124 E. coli K12 CAG626 3/7/2018
E6400 ProtoScript® Taq RT-PCR Kit 1/5/2015 E5310, E5315
E8030 Anti-GLuc Antibody 9/11/2013 E8032
E8102 Ph.D.™-7 Phage Display Peptide Library 1/1/2017 E8100
E2620 PicoPLEX™ WGA Kit 1/1/2017
E8121 Ph.D.™-C7C Phage Display Peptide Library 1/1/2017 E8120
E6550 AMV First Strand cDNA Synthesis Kit 1/1/2017 M0277
E3309 BioLux® Cypridina Luciferase Assay Kit 8/2/2018
E5300 AMV LongAmp™ Taq RT-PCR Kit 1/5/2015 E5310, E5315
E6114 NEBNext® mRNA Library Prep Set for SOLiD™ 1/1/2013
E4125 E. coli K12 CAG629 3/7/2018
E6110 NEBNext® mRNA Library Prep Master Mix Set for Illumina® 2/17/2017 E7760, E7770
E4122 E. coli K12 TB1 3/7/2018
E7350 NEBNext® Singleplex Oligos for Illumina® 1/2/2019
E4126 E. coli K12 PR1031 3/7/2018
E4107 E. coli K12 JM109 3/7/2018
E4129 E. coli K12 UT5600 3/7/2018
E4154 E. coli K12 ER2420/pBeloBAC11 3/7/2018
E4121 E. coli K12 ER2507 3/7/2018
E4103 E. coli K12 ER2267 3/7/2018
E6070 NEBNext® DNA Library Prep Master Mix Set for 454™ 1/2/2019
E4151 E. coli K12 ER2420/pACYC177 3/7/2018
E4152 E. coli K12 ER2420/pACYC184 3/7/2018
E4109 E. coli K12 ER2925 3/7/2018
E4108 E. coli K12 NM522 3/7/2018
E4106 E. coli K12 JM101 3/7/2018
E4128 E. coli K12 KS1000 3/7/2018
E4102 E. coli K12 ER1821 3/7/2018
E4127 E. coli K12 ER2508 3/7/2018
E4153 E. coli K12 POP2136/pFOS1 3/7/2018
E6116 NEBNext® mRNA Library Prep Master Mix Set for 454™ 5/12/2014
E6080 NEBNext® Quick DNA Library Prep Reagent Set for 454™ 3/18/2014
E6060 NEBNext® DNA Library Prep Set for SOLiD™ 2/6/2014
E3320 RNase Contamination Assay Kit 1/1/2016
E9200 CLIP-Cell™ Starter Kit 9/14/2017
E6160 NEBNext® Small RNA Library Prep Set for SOLiD™ 1/19/2016
E3300 BioLux® Gaussia Luciferase Assay Kit 8/2/2018
E3308 BioLux® Gaussia Luciferase Flex Assay Kit 1/5/2015
E3314 BioLux® Cypridina Luciferase Starter Kit 1/5/2015
E8023 Anti-GLuc Antibody 8/2/2018
E8038 Anti-MBP Monoclonal Antibody (HRP conjugated) 1/2/2018 E8032
E9230 CLIP-Surface™ Starter Kit 9/14/2017
M0233 SET7 Methyltransferase 1/2/2018
M0229 Human DNA (cytosine-5) Methyltransferase (Dnmt3A) Amino-terminal Ab 1/1/2013
M0228 HEN1 miRNA Methyltransferase 1/1/2016
M0231 Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Ab 1/5/2015
M0221 Human PRMT1 Methyltransferase 1/2/2018
M0232 Human DNA (cytosine-5) Methyltransferase (Dnmt3B) Carboxy-terminal Ab 1/1/2013
M0483 OneTaq® 2X Master Mix with GC Buffer 1/2/2018
M0321 Taq DNA Polymerase with ThermoPol® II (Mg-free) Buffer 1/1/2016 M0320
M0487 OneTaq® Quick-Load® 2X Master Mix with GC Buffer 1/2/2018 M0489
M0290 Alkaline Phosphatase, Calf Intestinal (CIP) 6/24/2019 M0525
M0326 Crimson LongAmp™ Taq DNA Polymerase 1/5/2015 M0323
M0333 Therminator™ III DNA Polymerase 1/1/2013
M0266 Therminator™ II DNA Polymerase 1/1/2013
M0428 SET8 Methyltransferase 1/2/2018
M0260 9°Nm™ DNA Polymerase 1/1/2013
M0508 Quick Dephosphorylation Kit 7/16/2019 M0525
M0241 hOGG1 6/15/2017 M0240
M0306 DNA Gyrase (E. coli) 9/14/2017
M0324 Crimson Taq DNA Polymerase 1/5/2015 M0486
M2561 TransPass™ V 1/1/2013
M2563 TransPass™ P Protein Transfection Reagent 1/1/2013
M2554 TransPass™ D2 Transfection Reagent 1/1/2013
M2402 Tth RecA 1/27/2016
M2552 TransPass™ R2 Transfection Reagent 1/1/2013
M0334 Therminator™ γ DNA Polymerase 1/1/2013
M2557 TransPass™ COS/293 Transfection Reagent 1/1/2013
M2558 TransPass™ HUVEC Transfection Reagent 1/1/2013
M0235 G9a Methyltransferase 12/1/2016
M0393 Apyrase 1/5/2015 M0398
M0641 Cas9 Nuclease NLS, S. pyogenes 10/1/2016 M0646
M0394 ATP Sulfurylase 1/2/2019
M2553 TransPass™ D1 Transfection Reagent 1/1/2013
M0325 Crimson Taq DNA Polymerase with (Mg-free) Buffer 1/5/2015 M0486
N3237 Mass DNA Ladder 12/3/2014
N3538 LITMUS 38i Vector 1/1/2013
N0350 Low Range PFG Marker 12/10/2014
N3528 LITMUS 28i Vector 1/5/2015 N3041
N3551 MidRange I PFG Marker 1/5/2015
N9216 pCLIPf-NK1R Control Plasmid 1/1/2013
N9217 pCLIPf-Cox8A Control Plasmid 1/1/2013
N8082 pGLuc-Basic 2 Vector 8/2/2018
N8081 pCMV-GLuc 2 Control Plasmid 8/2/2018
N0321 pCMV-CLuc 2 Control Plasmid 8/2/2018
N1500 NEBlot® Kit 5/13/2013
N3640 LITMUS™ U 1/1/2013
N0316 M13KE Phage 1/1/2017 E8101
N8084 pTK-GLuc Vector 1/1/2017
N2101 siRNA Marker 1/5/2015
N0474 Quick-Load® Low Molecular Weight DNA Ladder 4/28/2016 N0557
N3240 Reverse Mass DNA Ladder 1/5/2015
N0475 Quick-Load® PCR Marker 1/5/2015 N3234
N0473 Quick-Load® 50 bp DNA Ladder 4/28/2016 N0556
N0322 pTK-CLuc Vector 1/1/2017
N0324 pCLuc Mini-TK 2 Vector 1/1/2017
N0318 pSV40-CLuc Control Plasmid 8/2/2018
N0323 pSV40-GLuc Control Plasmid 8/2/2018
N0471 DNA Gyrase (E. coli) Substrate 10/6/2015
N7001 CDP-Star® Reagent 11/19/2013
N7554 Biotinylated 2-Log DNA Ladder (0.1-10 kb) 8/28/2014
N7022 Biotinylated Alkaline Phosphatase 1/1/2012
N3552 MidRange II PFG Marker 1/1/2013
N0317 pCLuc-Basic 2 Vector 8/2/2018
N9320 pMCP-GPI Control Plasmid 1/1/2013
N9218 pCLIPf-H2B Control Plasmid 1/5/2015
N4007 CpG Methylated HeLa Genomic DNA 1/2/2018
N4005 CpG Methylated NIH 3T3 Mouse Genomic DNA 1/5/2015
N3745 pKLCF-c Vector 1/5/2015
N7020 Phototope®-Star Detection Kit 4/16/2013
N3740 pKLAC1 Vector 1/1/2017
N3743 pKLCF-n Vector 1/5/2015
N3034 pKLAC1-GLuc Control Plasmid 1/1/2013
N3746 pKLCF-n Vector 1/5/2015
N3744 pKLMF-FX Vector 1/5/2015
N3742 pKLAC2 Vector 1/2/2018
N3741 pKLAC1-malE Control Plasmid 1/1/2013
N4002 CpG Methylated Jurkat Genomic DNA 1/2/2018
N9184 pSNAPf -ADRβ2 Control Plasmid 1/5/2015
N9186 pSNAPf-H2B Control Plasmid 1/5/2015
N9185 pSNAPf-Cox8A Control Plasmid 1/1/2013
N9322 pACP-tag(m)-2 Vector 1/5/2015
N5502 pNEB206A Linearized Vector 1/1/2017
N8114 pMAL™-c5X-His Vector 1/5/2015
N3051 pNEB193 Vector 1/1/2013
N8113 pMAL-p5G Vector 1/1/2013
N0417 pLox2+ (linearized) 1/1/2016
N8111 pMAL-p5E Vector 1/1/2013
N8101 pMAL-pIII Vector 1/5/2015
N4001 Jurkat Genomic DNA 1/5/2015
N8112 pMAL-c5G Vector 1/1/2013
N9317 pMCP-tag(m) Vector 1/5/2015
N8110 pMAL-c5E Vector 1/5/2015
N9321 pACP-ADRβ2 Control Plasmid 1/1/2013
N4004 NIH 3T3 Mouse Genomic DNA 1/1/2017
N8086 pGLuc Mini-TK 2 Vector 1/1/2017
N5504 Universal USER® Cassette 1/1/2013
N4003 5-Aza-dc Treated Jurkat Genomic DNA 1/2/2008
N2100 Fluorescein-siRNA Transfection Control 1/1/2013
N0345 Yeast Chromosome PFG Marker 12/10/2014
N7551 5X Labeling Mix 1/1/2013
P7704 Unstained Protein Standard, Broad Range (10-200 kDa) 5/1/2019 P7717
P5208 Plasmodium falciparum chitinase (PfCHT1) 1/1/2013
P0755 Protein Phosphatase Inhibitor 2 (I-2) 1/5/2015
P7708 Prestained Protein Marker, Broad Range (7-175 kDa) 7/28/2014
P8102 Proteinase K 8/30/2013 P8107
P7710 Prestained Protein Ladder, Broad Range (10-230 kDa) 6/13/2014 P7718
P6039 Protein Deglycosylation Mix 10/31/2016 P6044
P0754 Protein Phosphatase 1 (PP1) 11/24/2015
P0759 Sodium Fluoride (Fluoride) 1/2/2018 P0758
P6080 p42 MAP Kinase (MAPK) 1/1/2017
P6050 Abl Protein Tyrosine Kinase (Abl) 1/1/2013
P5206 Onchocerca volvulus chitinase (OvCHT1) 1/1/2013
P0738 Heparin Hexasaccharide MS Standard 6 1/5/2015
P0739 Heparin Hexasaccharide MS Standard 7 1/5/2015
P9311 CLIP-tag™ Purified Protein 1/1/2013
P6030 Casein Kinase I (CK1) 1/1/2017
P6060 Calmodulin-dependent Protein Kinase II (CaMKII) 1/1/2017
P5205 Brugia malayi chitinase (BmCHT1) 1/1/2013
P7706 Blue Prestained Protein Standard, Broad Range (11-190 kDa) 5/1/2019 P7718
P6040 Glycogen Synthase Kinase 3 (GSK-3) 1/1/2017
P8075 Genenase I 1/1/2013
P0732 β-N-Acetylglucosaminidase 12/15/2016 P0744
P0728 α2-3 Neuraminidase 1/1/2017 P0743
P0730 β1-4 Galactosidase 12/15/2016 P0745
P7702 Unstained Protein Marker, Broad Range (2-212 kDa) 2/26/2016 P7717
P7703 Unstained Protein Ladder, Broad Range (10-250 kDa) 7/14/2016 P7717
P7709 ColorPlus™ Prestained Protein Marker, Broad Range (7-175 kDa) 6/13/2014 P7719
P6020 CDK1-cyclin B 1/1/2017
P7712 Color Prestained Protein Standard, Broad Range (11–245 kDa) 5/1/2019 P7719
P0752 T-Cell Protein Tyrosine Phosphatase (TC PTP) 1/1/2013
P7711 ColorPlus™ Prestained Protein Ladder, Broad Range (10-230 kDa) 6/13/2014 P7719
R3535 BsaI-HF® 11/8/2018 R3733
R0657 PsiI 7/1/2019 R0744
R0634 AleI 12/18/2018 R0685
S9101 SNAP-Cell® 360 1/1/2017
S9142 SNAP-Surface® 782 10/3/2014
S9131 SNAP-Surface® 532 1/1/2013
S9146 SNAP-Vista® Blue 1/1/2013
S6654 Anti-Chitin Binding Domain Serum 1/1/2013
S9222 BC-PEG-NH2 1/5/2015
S9155 BG-GLA 1/1/2013
S1560 polyA Spin™ mRNA Isolation Kit 3/31/2020 E7490
S1408 Oligo (dT)25 Cellulose Beads 3/31/2020 E7490
S1510 2-Tube Magnetic Separation Rack 1/5/2015
S9147 SNAP-Vista® Green 1/2/2020 S9103, S9124, S9129
S9107 SNAP-Cell® Fluorescein 5/28/2019 S9103, S9104
S9126 SNAP-Surface® 425 1/1/2013
S9137 SNAP-Surface® 647 9/10/2013
S9235 CLIP-Vista Green 1/1/2013
S9349 CoA 547 9/14/2017
S9218 CLIP-Cell™ Fluorescein 1/1/2013
S9352 CoA-SH 10/3/2014
S9348 CoA 488 9/14/2017
S9216 CLIP-Cell™ 430 1/1/2013
S9215 CLIP-Cell™ 360 1/1/2013
S9350 CoA 647 9/14/2017
S9351 CoA-Biotin 9/14/2017
S9149 CBG-NH2 1/1/2013
S9139 SNAP-Surface® 682 10/3/2014
S9237 BC-GLA-NHS 1/5/2015
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