Goat Anti-Rabbit IgG Magnetic Beads

Description

An affinity matrix for the small-scale immunomagnetic separation and purification of rabbit IgG's. Goat Anti-Rabbit IgG is covalently coupled to a nonporous superparamagnetic particle. This secondary antibody binds the heavy chain of all rabbit IgG subclasses and is suitable for immunoassays that employ a rabbit IgG primary polyclonal antibody. Cell separations and sorting can be accomplished using a rabbit IgG antibody to defined cell surface antigens.

Supplied as a 1 ml suspension in PBS Buffer (pH 7.4), containing 0.05% Tween 20, 0.1% BSA and 0.05% NaN3.

Properties and Usage

Storage Temperature

4°C

Storage Conditions

0.05% Tween® 20
0.05% NaN3
1X PBS
pH 7.4 @ 25°C

Binding Capacity

5 μg Rabbit lgG/mg Goat Anti-Rabbit IgG Magnetic Beads

Can be Regenerated

yes

Notes

  1. The amount of antibody required for optimal coating of the beads will vary with the PAb affinity and the antigen density on the cell surface. Optimal experimental conditions should be determined by titration on an individual basis.

Protocols

  1. Goat Anti-Rabbit IgG Magnetic Beads Protocol

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.