Oligo d(T)25 Magnetic Beads

Description

Oligo d(T)25 Magnetic Beads are a 1 μm polymer based affinity matrix for the small-scale isolation of mRNA from crude cell lysates and tissue. The isolation occurs through the hybridization of covalently coupled oligo d(T)25 to the poly(A) region present in most eukaryotic mRNA. Applications include direct mRNA isolation following lysis and second-round purification of previously isolated total RNA. The magnetic separation technology is scalable and permits elution of intact mRNA in small volumes eliminating the need for precipitation of the isolated mRNA. Beads can be reused up to three times and the researcher has the option of eluting the isolated mRNA or using the bound d(T)25 as a primer in a first-strand cDNA reaction.

Supplied as a 5 mg/ml suspension in phosphate buffered saline (PBS buffer) (pH 7.4), containing 0.05% Tween® 20 and 0.05% NaN3.

Recommended Buffers
  • Lysis/Binding Buffer
  • Wash Buffer I
  • Wash Buffer II
  • Low Salt Buffer
  • Elution Buffer

Properties and Usage

Storage Temperature

4°C

Storage Conditions

0.1% Tween® 20
0.02% NaN3
1X PBS
pH 7.4 @ 25°C

Protocols

  1. cDNA synthesis in oligo (dT)25 magnetic beads (S1419)

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.