Oligo d(T)25 is covalently coupled to 1 μm superparamagnetic particle through a linkage that is stable and leak resistant over a wide pH range, thereby permitting the small-scale isolation of mRNA from crude cell lysates and tissue. The isolation occurs through the hybridization of covalently coupled oligo d(T)25 to the poly(A) region present in most eukaryotic mRNAs.
• Allows for direct mRNA isolation following lysis and second-round purification of previously isolated total RNA
• The magnetic separation technology is scalable and permits elution of intact mRNA in small volumes, thereby eliminating the need for precipitation of the isolated mRNA
• Beads can be reused up to three times
• The isolated mRNA can either be eluted or the bound d(T)25 can be used as a primer in a first-strand cDNA reaction
Supplied as a 5 mg/ml suspension in phosphate buffered saline (PBS buffer) (pH 7.4), containing 0.05% Tween® 20 and 0.02% NaN3.
Lysis/Binding Buffer: 100 mM Tris-HCl, pH 7.5, 500 mM LiCl, 0.5% Lithium Dodecyl Sulfate (LiDS), 1 mM EDTA, 5 mM DTT
Wash Buffer I: 20 mM Tris-HCl, pH 7.5, 500 mM LiCl, 0.1% LiDS, 1 mM EDTA, 5 mM DTT
Wash Buffer II: 20 mM Tris-HCl, pH 7.5, 500 mM LiCl, 1 mM EDTA
Low-Salt Buffer: 20 mM Tris-HCl, pH 7.5, 200 mM LiCl, 1 mM EDTA
Elution Buffer: 20 mM Tris-HCl, pH 7.5, 1 mM EDTA
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
The following reagents are supplied with this product:
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