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OneTaq® DNA Polymerases


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An optimized blend of Taq and Deep Vent® DNA polymerases, OneTaq® and OneTaq® Hot Start DNA Polymerases offer robust amplification across a wide range of templates. The 3′–5′ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robustness of Taq, and the hot start formulation combines convenience with decreased interference from primer dimers and secondary products. Additionally, OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template's GC content. Both polymerases are available in master mix and Quick-Load® master mix formats.

OneTaq Hot Start DNA Polymerase

In contrast to chemically modified or antibody-based hot start polymerases, NEB's OneTaq Hot Start utilizes aptamer technology. This unique modified oligonucelotide binds to the polymerase through non-covalent interactions, blocking polymerase activity at temperatures below 45°C. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature.

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Comparison of OneTaq Products to Other Commercially Available Polymerases

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Amplification of a selection of high GC human genomic DNA targets demonstrates OneTaq performance. All polymerases were cycled according to manufacturer’s recommendations, including the use of additives to enhance the amplification of targets with high GC content. Yield (dot size) and purity (color) of reaction product were quantified from triplicate reactions on a Perkin Elmer LabChip®. A large, dark green dot represents the highest yield and purity.

 

OneTaq Quick-Load 2X Master Mix with Standard Buffer

OneTaq Quick-Load 2X Master Mix with Standard Buffer contains an optimized blend of Taq and Deep Vent® DNA Polymerases in a convenient master mix format containing two commonly used tracking dyes for DNA gels. Request a free sample here.

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Amplification of a selection of sequences with varying GC content from human and C. elegans genomic DNA using OneTaq DNA Polymerase. Amplicon sizes are indicated next to gel, and GC content is indicated above gel. Marker M is the 1 kb DNA Ladder (NEB #N3232).

 

OneTaq Quick-Load DNA Polymerase

For direct and fast agarose gel loading after routine PCR, such as genotyping and colony PCR, OneTaq Quick-Load DNA Polymerase is available. The product is supplied with a green 5X OneTaq Quick Load Reaction Buffer in addition to the colorless 5X OneTaq Reaction Buffer.

Robust Performance of OneTaq Quick-Load DNA Polymerase

Amplification of a variety of DNA targets (human and viral templates) demonstrates strong performance of the OneTaq Quick-Load DNA Polymerase. Product yield (bars, left axis) and purity (circles, right axis) were calculated via microfluidic analysis from triplicate reactions after 30 cycles of PCR. Standard deviation is indicated by error bars (yield) or shaded bands (purity). GoTaq was cycled according to manufacturer’s recommendations.

 

One or more of these products are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc.

AmpliTaq Gold® and FastStart™ are trademarks of Roche Molecular Systems, Inc. DreamTaq® and Platinum™ are trademarks of Thermo Fisher Scientific, Inc. GoTaq® is a registered trademark of Promega Corporation.  HotStarTaq® is a registered trademark of Qiagen GmbH, LLC. iTaq™ is a trademark of Bio-Rad Laboratories, Inc. JumpStart™ is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Ex Taq® and Titanium™ are trademarks of Takara Holding, Inc.

 

 

Choose Type:

FAQs for OneTaq® DNA Polymerases
Protocols for OneTaq® DNA Polymerases
Advantages
  • Exceptional performance in endpoint PCR across a wide range of template
  • Robust yields with minimal optimization
  • Convenient product formats (stand-alone enzyme, master mixes, and Quick-Load® formats)
  • Hot start version allows room temperature reaction setup and does not require a separate activation step 
  • Compatible with standard Taq protocols
Applications
  • High sensitivity PCR
  • High throughput PCR
  • Routine PCR
  • GC-rich PCR
  • AT-rich PCR
  • Primer extension
  • Colony PCR
  • Long PCR (up to ~6 kb genomic)
DNA Polymerase Selection Chart
NEB offers a guidelines for choosing the correct DNA polymerase for your application by providing a list of specific properites.
Several factors govern which polymerase should be used in a given application, including: 

Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template? 

Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end? 

Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable? 

Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?

OneTaq 2X Master Mix with GC Buffer
Amplification of a selection of sequences with varying GC content from human genomic DNA using OneTaq 2X Master Mix with GC Buffer. GC content is indicated above gel. Marker M is the 1 kb DNA Ladder (NEB# N3232).
OneTaq 2X Master Mix with Standard Buffer
Amplification of a selection of sequences with varying GC content from human and C. elegans genomic DNA using OneTaq 2X Master Mix with Standard Buffer. Amplicon sizes are indicated next to gel, and GC content is indicated above gel. Marker M is the 1 kb DNA Ladder (NEB# N3232).
OneTaq Buffer Recommendations
NEB recommends choosing your OneTaq Buffer based on amplicon % GC content.
OneTaq Hot Start vs Other Commercially Available Hot Start Polymerases
Comparison of OneTaq Hot Start DNA Polymerases to Other Commercially Available Hot Start Polymerases
Reactions containing high GC human genomic DNA templates were set up at room temperature. PCR experiments included 30 cycles. Purity (A) and Yield (B) were calculated via microfluidic analysis from triplicate reactions. OneTaq polymerases were used with GC Buffer. Some OneTaq reactions also contained High GC Enhancer (striped bars). Competitor polymerases were cycled according to manufacturer's recommendations and included GC enhancers when supplied (striped bars).
OneTaq vs Other Commercially Available Polymerases
Comparison of OneTaq DNA Polymerase to Other Commercially Available Polymerases (non-hot start)
Amplification of a selection of high GC human genomic DNA templates demonstrates OneTaq performance. PCR experiments included 30 amplification cycles. Purity (A) and Yield (B) were calculated via microfluidic analysis from triplicate reactions. Competitor polymerases were cycled according to manufacturer's recommendations.
PCR Selection Tool
Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme technology and bring unparalleled confidence to your experiments.
Recommended Time for Enzyme Activation
Recommended time for enzyme activation of commercially available Hot Start
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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