OneTaq® Buffer Recommendations
NEB recommends choosing your OneTaq buffer based on amplicon % GC content.
| AMPLICON % GC CONTENT |
RECOMMENDED DEFAULT BUFFER |
OPTIMIZATION NOTES |
|---|---|---|
| <50% GC | OneTaq Standard Reaction Buffer* | Adjust annealing temperature, primer/ template concentration, etc., if needed. |
| 50–65% GC | OneTaq Standard Reaction Buffer* | OneTaq GC Reaction Buffer can be used to enhance performance of difficult amplicons. |
| >65% GC | OneTaq GC Reaction Buffer* | OneTaq GC Reaction Buffer with 10–20% OneTaq High GC Enhancer can be used to enhance performance of difficult amplicons. |
* Mg-free formulations are also available.
