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OneTaq® Buffer Recommendations

NEB recommends choosing your OneTaq buffer based on amplicon % GC content.

AMPLICON %
GC CONTENT		 RECOMMENDED
DEFAULT BUFFER		 OPTIMIZATION NOTES
<50% GC OneTaq Standard Reaction Buffer*  Adjust annealing temperature, primer/
template concentration, etc., if needed.
50–65% GC OneTaq Standard Reaction Buffer* OneTaq GC Reaction Buffer can be
used to enhance performance of difficult
amplicons.
>65% GC OneTaq GC Reaction Buffer* OneTaq GC Reaction Buffer with
10–20% OneTaq High GC Enhancer
can be used to enhance performance of
difficult amplicons.

* Mg-free formulations are also available.