3´-Biotin-GTP

3'-Desthiobiotin-GTP or 3'-Biotin GTP are guanosine triphosphate (GTP) analogs which are modified at their 3’ position with desthiobiotin or biotin, respectively. When used with the Vaccinia Capping System, (NEB #M2080) these reagents enable affinity tagging of RNA triphosphate ends. Tagged RNAs are enriched by binding to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421). Desthiobiotin-tagged RNAs can be eluted with free biotin. This approach is used in Cappable-seq, a method developed at NEB for directly enriching the 5’-ends of primary transcripts (1).
Catalog # Concentration Size List Price Your Price Quantity
N0760S 5 mM 0.5 µmol $310.00
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  • Product Information


    BT-GTP


    Supplied in: Ultrapure water as a Triethylammonium salt at pH 7.0
    Molecular Formula: C31H50N11O19P3S
    Molecular Weight: 1005.78 g/mol
    Extinction Coefficient: λ260 = ~11,700 Lmol-1 cm-1
    Product Categories:
    Nucleotide Solutions Products,
    RNA Synthesis Products
    Applications:
    Cappable-seq,
    RNA Modification,
    In vitro Synthesis (IVT)
    ,
    PCR
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