3´-Biotin-GTP

Description

3´-Biotin-GTP can be used with the Vaccinia Capping System (NEB #M2080),to cap 5´ ends of tri- or di- phosphorylated RNA (1). This enables capture of the RNA 5´ ends using streptavidin.

BT-GTP


Supplied in: Ultrapure water as a Triethylammonium salt at pH 7.0
Molecular Formula: C31H50N11O19P3S
Molecular Weight: 1005.78 g/mol
Extinction Coefficient: λ260 = ~11,700 Lmol-1 cm-1

References

  1. Boutard, M. et al. (2016). Nature Communications. DOI: 7:13783
  2. Ettwiller, L. et al. (2016). BMC Genomics. DOI: 17:199

Interactive Tools

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • Functional Testing (Capping, 3'-Biotin-GTP):
    The product is tested in a RNA capping reaction using the Vaccinia capping enzyme.
  • Physical Purity (HPLC):
    The purity of the product is determined by HPLC analysis.
  • RNase Assay (Extended Digestion):
    The product is tested in a reaction containing a fluorescent RNA substrate. After incubation >90% of the substrate RNA remains intact as determined by gel electrophoresis.

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]