Deoxynucleotide (dNTP) Solution Set

Catalog #SizeConcentrationPriceQtyAdd to Cart
N0446S25 μmol of each100 mM$163.00Add to Cart
  
Categories:
Nucleotide Solution Products,
Nucleotide Solution Products,
Reverse Transcriptases & RT-PCR Products
Applications:
A-tailing,
DNA End Modification,
DNA Repair

Description

The Deoxynucleotide (dNTP) Solution Set contains 0.25 ml of each 100 mM ultrapure nucleotide as four separate solutions (dATP, dCTP, dGTP and dTTP).

Supplied in: Ultrapure water as a sodium salt at pH 7.5.

Kit Components

The following reagents are supplied with this product:

Store at (°C)Concentration
dATP Solution-20100 mM
dCTP-20100 mM
dGTP-20100 mM
dTTP-20100 mM

Properties and Usage

Storage Temperature

-20°C

Notes

  1. Supplied in ultrapure water as a sodium salt at pH 7.5 .
  2. Can be diluted using sterile distilled water or TE (10mM Tris-HCl, 1mM  EDTA pH 7.5).

FAQs

  1. What is supplied with the Deoxynucleotide Solution Set?
  2. How much is in each vial of the Deoxynucleotide Solution Set?
  3. What is the concentration of dNTPs in the Deoxynucleotide Solution Set?
  4. Which polymerases can the Deoxynucleotide Solution Set be used with?
  5. How does this product differ from the Deoxynucleotide Solution Mix (NEB# N0447)?
  6. How stable are the nucleotides in the Deoxynucleotide Solution Set?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Quality Control

Quality Assurance Statement

  • The purity of each deoxynucleotide is ≥ 99% as determined by HPLC analysis.

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Non-Specific DNase Activity (16 hour):
    The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
  • PCR Amplification (dNTPs):
    The dNTPs are tested in a polymerase chain reaction (PCR) using Taq DNA Polymerase, a control template and specific primers, resulting in the expected product.
  • Phosphatase Activity (PNPP):
    The product is tested in a reaction containing a p-nitrophenyl phosphate (PNPP), a chromogenic substrate for most phosphatases. After incubation the percent degradation is determined by spectrophotometric analysis of released p-nitrophenol at 405 nm.

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.