Lambda PFG Ladder

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
N0341S     -20       Lambda PFG Ladder (GelSyringe Dispenser) N0341SVIAL -20 1 x 50 gel lanes 50 µg/ml

Companion Products

• λ DNA-Mono Cut Mix
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)
• MidRange PFG Marker

1. The photograph represents the pulsed field gel separation of Lambda Ladders using a CHEF apparatus. The 1% agarose gel was run at 4.5 volts/cm using ramped pulse times from 5 to 120 seconds for 48 hours at 15°C in 0.5X TBE (50 mM Tris-HCl, 50 mM boric acid, 1 mM EDTA) made with Milli-Q™ water allowing resolution of 15 to 21 Lambda Ladder bands.
2. Recommended plug sizes are from 5-10 µl. A 10 µl plug (one small graduation on the GelSyringe volume scale) contains approximately 0.5 µg of DNA. Each GelSyringe yields 50+ plugs.
3. Extrude agarose from GelSyringe carefully and slice plugs from the end with a sharp blade. One plug is sufficient for one lane of a gel. Place the plug at the front of the well and seal with molten agarose just above gelling temperature (~42–45°C). Allow no bubbles to form.
4. Melting plugs will cause denaturation of concatemers.
5. Never attach the agarose plugs to the gel comb before the gel is poured. Heat from the solidifying gel will cause the Lambda concatemers to denature.

1. Burke-Aguaro, D. unpublished observation.
2. Ellard, J., Greci, J. and Davis, T.B. unpublished observation.

1. Why am I seeing poor results when running the PFG marker on my pulse field gel?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• N0341S_v1_0021606
• N0341S_v1_0041706
• N0341S_v1_10017974
• N0341S_v1_10040190
• N0341S_v1_10058457
• N0341S_v1_10081430
• N0341S_v1_10107782
• N0341S_v1_10141644
• N0341S_v1_10175318
• N0341S_v1_10180292

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Lambda PFG Ladder

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.