Hot Start Taq DNA Polymerase
The industry standard with hot start performance
- Ideal for routine PCR applications and molecular diagnostics
- High specificity and sensitivity
- Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup
Choose the Right DNA Polymerase for PCR
5 Tips for Setting Up Your PCR
Why is Tm Important in Primer Design?
Overview of PCR
Ordering InformationDid you know this product can be customized or purchased in larger volumes? Submit an inquiry to find out more about customization options.
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5´→3´ polymerase activity (1)(2)(3)and a 5´ flap endonuclease activity(4)(5).
It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.
Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.
The following reagents are supplied with this product:
Store at (°C) Concentration Standard Taq Reaction Buffer Pack -20 10 X
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