Hot Start Taq DNA Polymerase

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0495S     -20       Hot Start Taq DNA Polymerase M0495SVIAL -20 1 x 0.04 ml 5,000 units/ml   Standard Taq Reaction Buffer Pack B9014SVIAL -20 1 x 1.5 ml 10 X
M0495L     -20       Hot Start Taq DNA Polymerase M0495LVIAL -20 1 x 0.2 ml 5,000 units/ml   Standard Taq Reaction Buffer Pack B9014SVIAL -20 3 x 1.5 ml 10 X

Application Features

• High-specificity PCR
• Routine PCR
• Microarray Analysis
• Colony PCR

Companion Products

• Deoxynucleotide (dNTP) Solution Set
• Deoxynucleotide (dNTP) Solution Mix
• Hot Start Taq 2X Master Mix
• Magnesium Chloride (MgCl₂) Solution
• Standard Taq Reaction Buffer Pack

1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
2. Kaledin, A.S., Slyusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
6. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
7. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res. 18, 7465.

1. PCR Using Hot Start Taq DNA Polymerase (M0495)
2. A-Tailing with Taq Polymerase

• Activity of Restriction Enzymes in PCR Buffers

• PCR Selector
• Tm Calculator

1. Does Hot Start Taq DNA Polymerase require an extended initial incubation to activate the polymerase?
2. How should I set up a PCR using Hot Start Taq DNA Polymerase?
3. Can Hot Start Taq DNA Polymerase be used in other buffers?
4. What type of DNA ends result from a primer extension reaction or a PCR reaction using Hot Start Taq DNA Polymerase?
5. When should Hot Start Taq DNA Polymerase be used in a primer extension reaction or for PCR?
6. Will the 5'→3' flap endonuclease activity of Hot Start Taq DNA Polymerase degrade primers?
7. Can Hot Start Taq DNA Polymerase be used for nick translation?
8. How should I determine an appropriate annealing temperature for my reaction?
9. What is the longest amplicon that can be obtained with Hot Start Taq DNA Polymerase?
10. Can Hot Start Taq DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?

• Using aptamers to control enzyme activities: Hot Start Taq and beyond

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0495S_L_v1
• M0495S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0495S_L_v1_0011509
• M0495S_L_v1_0011603
• M0495S_L_v1_0031608
• M0495S_L_v1_0031703
• M0495S_L_v1_0031709
• M0495S_L_v1_0031803
• M0495S_v1_10011369
• M0495L_v1_10015893
• M0495L_v1_10019647
• M0495S_v1_10015904
• M0495L_v1_10032782
• M0495L_v1_10036030
• M0495L_v1_10040039
• M0495S_v1_10042556
• M0495L_v1_10047883
• M0495S_v1_10047884
• M0495L_v1_10054111
• M0495L_v1_10055901
• M0495L_v1_10058437
• M0495L_v1_10062497
• M0495S_v1_10061071
• M0495L_v2_10108735
• M0495S_v2_10120886
• M0495L_v2_10121217
• M0495S_v2_10141009
• M0495L_v2_10150849
• M0495S_v2_10162272
• M0495L_v2_10164439
• M0495S_v2_10164440
• M0495S_v2_10169256
• M0495L_v2_10168301

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Hot Start Taq DNA Polymerase
• Standard Taq Reaction Buffer Pack

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Notice to Purchaser: Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the Hot-Start Taq DNA Polymerase for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com. 

Trademarks

NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
PRIMERSELECT™ is a trademark of DNAStar, Inc., Madison, Wisconsin, USA.