Hot Start Taq DNA Polymerase

Application Features

• High-specificity PCR
• Routine PCR
• Microarray Analysis
• Colony PCR

Companion Products

• Standard Taq Reaction Buffer Pack
• Magnesium Chloride (MgCl₂) Solution
• Hot Start Taq 2X Master Mix
• Deoxynucleotide (dNTP) Solution Set
• Deoxynucleotide (dNTP) Solution Mix

1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
2. Kaledin, A.S., Slyusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
6. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
7. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res. 18, 7465.

1. PCR Using Hot Start Taq DNA Polymerase (M0495)
2. A-Tailing with Taq Polymerase

• Activity of Restriction Enzymes in PCR Buffers

• PCR Selection Chart

• PCR Selector
• Tm Calculator

1. Does Hot Start Taq DNA Polymerase require an extended initial incubation to activate the polymerase?
2. How should I set up a PCR using Hot Start Taq DNA Polymerase?
3. Can Hot Start Taq DNA Polymerase be used in other buffers?
4. What type of DNA ends result from a primer extension reaction or a PCR reaction using Hot Start Taq DNA Polymerase?
5. When should Hot Start Taq DNA Polymerase be used in a primer extension reaction or for PCR?
6. Will the 5'→3' flap endonuclease activity of Hot Start Taq DNA Polymerase degrade primers?
7. Can Hot Start Taq DNA Polymerase be used for nick translation?
8. How should I determine an appropriate annealing temperature for my reaction?
9. What is the longest amplicon that can be obtained with Hot Start Taq DNA Polymerase?
10. Can Hot Start Taq DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?

Product Citation Tool

• Using aptamers to control enzyme activities: Hot Start Taq and beyond

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0495S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0495S_L_v1_0011509
• M0495S_L_v1_0011603
• M0495S_L_v1_0031608
• M0495S_L_v1_0031703
• M0495S_L_v1_0031709
• M0495S_L_v1_0031803
• M0495S_v1_10011369
• M0495L_v1_10015893
• M0495L_v1_10019647
• M0495S_v1_10015904
• M0495L_v1_10032782
• M0495L_v1_10036030
• M0495L_v1_10040039
• M0495S_v1_10042556
• M0495L_v1_10047883
• M0495S_v1_10047884
• M0495L_v1_10054111
• M0495L_v1_10055901
• M0495L_v1_10058437
• M0495L_v1_10062497
• M0495S_v1_10061071
• M0495L_v0_10065421
• M0495L_v0_10071586

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Hot Start Taq DNA Polymerase
• Standard Taq Reaction Buffer Pack

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Notice to Purchaser: Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 7,947,447; 8,404,830; 8,975,388 and their foreign equivalents). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based Hot-Start Taq DNA Polymerase for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based Hot Start Taq DNA Polymerase requires a license from New England Biolabs, Inc. Please contact [email protected] for more information.

Trademarks

NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.

PRIMERSELECT™ is a trademark of DNAStar, Inc., Madison, Wisconsin, USA.