Application Features

• High-specificity PCR
• Routine PCR
• Microarray Analysis
• Colony PCR

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X Standard Taq Reaction Buffer Pack

1X Standard Taq Reaction Buffer Pack
10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl₂
(pH 8.3 @ 25°C)

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
1X stabilizers
pH 7.4 @ 25°C

Heat Inactivation

No

Unit Assay Conditions

1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA.

Companion Products

• Standard Taq Reaction Buffer Pack
• Magnesium Chloride (MgCl₂) Solution
• Hot Start Taq 2X Master Mix
• Deoxynucleotide (dNTP) Solution Set
• Deoxynucleotide (dNTP) Solution Mix

1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
2. Kaledin, A.S., Slyusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
6. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
7. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res. 18, 7465.

1. Does Hot Start Taq DNA Polymerase require an extended initial incubation to activate the polymerase?
2. How should I set up a PCR using Hot Start Taq DNA Polymerase?
3. Can Hot Start Taq DNA Polymerase be used in other buffers?
4. What type of DNA ends result from a primer extension reaction or a PCR reaction using Hot Start Taq DNA Polymerase?
5. When should Hot Start Taq DNA Polymerase be used in a primer extension reaction or for PCR?
6. Will the 5'→3' flap endonuclease activity of Hot Start Taq DNA Polymerase degrade primers?
7. Can Hot Start Taq DNA Polymerase be used for nick translation?
8. How should I determine an appropriate annealing temperature for my reaction?
9. What is the longest amplicon that can be obtained with Hot Start Taq DNA Polymerase?
10. Can Hot Start Taq DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?

1. PCR Using Hot Start Taq DNA Polymerase (M0495)
2. A-Tailing with Taq Polymerase

• PCR Selection Chart

• Activity of Restriction Enzymes in PCR Buffers

• NEBioCalculator
• PCR Selector
• Tm Calculator

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0495S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0495S_L_v1_0011509
• M0495S_L_v1_0011603
• M0495S_L_v1_0031608
• M0495S_L_v1_0031703
• M0495S_L_v1_0031709
• M0495S_L_v1_0031803

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Hot Start Taq DNA Polymerase
• Standard Taq Reaction Buffer Pack

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Licenses

Notice to Purchaser: Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 5,475,096; 5,670,637; 5,696,249; 5,874,557; and 5,693,502). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based Hot Start Taq DNA Polymerase for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based Hot Start Taq DNA Polymerase requires a license from New England Biolabs, Inc. Please contact busdev@neb.com for more information

Certain applications in which this product can be used may be covered by third party patents or other intellectual property rights. It is the sole responsibility of the Buyers/Users of this product to determine whether they may be required to obtain any third party intellectual property rights (which may include, without limitation, a license to perform any patented application) depending upon the particular application in which the product is used.

Trademarks

NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
PRIMERSELECT™ is a trademark of DNAStar, Inc., Madison, Wisconsin, USA.