RecAf is His-tagged. Native RecA is also available as a separate product.

icon_recomb icon_NEBU icon_incTemp37 icon_HI_65
RecAf is a N-terminal 6X His tagged recombinant protein.  E. coli RecA is necessary for genetic recombination, reactions involving DNA repair and UV-induced mutagenesis. RecA promotes the autodigestion of the lexA repressor, umuD protein and lambda repressor. Cleavage of LexA derepresses more than 20 genes. In vitro studies indicate that in the presence of ATP, RecA promotes the strand exchange of single-strand DNA fragments with homologous duplex DNA. The reaction has three distinct steps: (i) RecA polymerizes on the single-strand DNA, (ii) the nucleo­protein filament binds the duplex DNA and searches for a homologous region, (iii) the strands are exchanged.

Ordering Information

  • 2 mg/ml
    200 μg
  • 2 mg/ml
    1,000 μg
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