Terminal transferase catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.
- Useful for Labeling of the 3′ ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP)
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Tested for the absence of endonucleases and exonucleases
Ordering InformationDid you know this product can be customized or purchased in larger volumes? Submit an inquiry to find out more about customization options.
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT. The 58.3 kDa enzyme does not have 5' or 3' exonuclease activity. The addition of Co2+ in the reacton makes tailing more efficient.
Product SourceAn E. coli strain that carries the cloned Terminal Transferase gene from calf thymus.
The following reagents are supplied with this product:
Store at (°C) Concentration CoCl2 -20 10 X Terminal Transferase Reaction Buffer Pack -20 10 X
- FAQs & Tech Tips
- Protocols & Manuals
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at firstname.lastname@example.org or 1-800-632-5227 x 8.