Terminal transferase catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.
- Useful for Labeling of the 3′ ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP)
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Tested for the absence of endonucleases and exonucleases
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT. The 58.3 kDa enzyme does not have 5' or 3' exonuclease activity. The addition of Co2+ in the reacton makes tailing more efficient.
Product SourceAn E. coli strain that carries the cloned Terminal Transferase gene from calf thymus.
The following reagents are supplied with this product:
Store at (°C) Concentration CoCl2 10X Terminal Transferase Reaction Buffer Pack 10X
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