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Ordering Information

  • 5,000 units/ml
    250 units
  • 5,000 units/ml
    1,250 units
  • Product Information
    Ribonuclease HII (RNase HII) is an endoribonuclease that preferentially nicks 5´ to a ribonucleotide within the context of a DNA duplex. The enzyme leaves 5´ phosphate and 3´ hydroxyl ends (1). RNase HII will also nick at multiple sites along the RNA portion of an Okazaki fragment.

    Product Source

    An E. coli strain containing a genetic fusion of the RNase HII gene (rnhB) from E. coli and the gene coding for maltose binding protein (MBP). Following affinity chromatography, RNase HII is cleaved from the fusion construct by Factor Xa and then purified away from both MBP and Factor Xa. RNase HII cleaved from MBP has four additional amino acids at its N-terminus (Ile-Ser-Glu-Phe).

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    ThermoPol® Reaction Buffer Pack -20 10 X
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