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  • NEBNext® Quick Ligation Module

    Description






    The NEBNext® Quick Ligation Module has been optimized to efficiently ligate DNA adaptors to blunt or dA-Tailed DNA fragments. The NEBNext Quick Ligation Module is provided as a master mix to maximize efficiency and convenience in DNA sample preparation workflows.

    Functional Validation

    Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a , .

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Quick T4 DNA Ligase
    NEBNext Quick Ligation Reaction Buffer5X

    Advantages and Features

    Features

    • Efficient – Ligates blunt DNA or dA-Tailed DNA
    • Convenient – Reactions are provided in master mix format to reduce steps during DNA sample prep workflows
    • Automation Friendly

    Applications

    • DNA Sample Preparation
    • Ligation of DNA adaptors to blunt or dA-Tailed DNA
    • Ligation of blunt or dA-Tailed DNA and cloning vectors

    Properties and Usage

    Storage Temperature

    -20°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What type of sample prep/library construction can I use the Quick Ligation Module reagents for?
    2. How much DNA can I blunt in one NEBNext Quick Ligation Reaction?
    1. NEBNext Quick Ligation Module Protocol (E6056)

    Selection Tools

    Usage Guidelines & Tips

    Troubleshooting Guides