NEBNext® Quick Ligation Module


The NEBNext® Quick Ligation Module has been optimized to efficiently ligate DNA adaptors to blunt or dA-Tailed DNA fragments. The NEBNext Quick Ligation Module is provided as a master mix to maximize efficiency and convenience in DNA sample preparation workflows.

Functional Validation

Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a DNA library on the Illumina sequencing platform.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Quick T4 DNA Ligase-20
NEBNext Quick Ligation Reaction Buffer-20

Advantages and Features


  • Efficient – Ligates blunt DNA or dA-Tailed DNA
  • Convenient – Reactions are provided in master mix format to reduce steps during DNA sample prep workflows
  • Automation Friendly


  • DNA Sample Preparation
  • Ligation of DNA adaptors to blunt or dA-Tailed DNA
  • Ligation of blunt or dA-Tailed DNA and cloning vectors

Properties and Usage

Storage Temperature



  1. What type of sample prep/library construction can I use the Quick Ligation Module reagents for?
  2. How much DNA can I blunt in one NEBNext Quick Ligation Reaction?


  1. NEBNext Quick Ligation Module Protocol (E6056)


The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

Selection Charts

Usage Guidelines & Tips

Troubleshooting Guides

Interactive Tools

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.