Nucleases
NEB offers a number of endonucleases and exonucleases that cleave DNA.
Exonucleases catalyze the removal of nucleotides in either the 5’ to 3’ or the 3’ to 5’ direction from single or double stranded DNA. Endonucleases cleave DNA nonspecifically and can be used to generate blunt ends for ligation or to shorten duplex DNA.
Learn more about common applications and properties of exonucleases and endonucleases.
References
Choose Type:
Protocols for Nucleases
- Protocol for T5 Exonuclease (M0363)
- Removal of Single-Stranded Extension Protocol using Mung Bean Nuclease (M0250)
- A Typical Exonuclease V Reaction (M0345)
- A Typical DNase I Reaction Protocol (M0303)
- In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386)
- T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
- Electroporation of Cas9 RNP (ribonucleoprotein) into adherent cells using the Neon® Electroporator
- Standard Reaction Protocol for PreCR Repair Mix
- Sequential Reaction Protocol for PreCR Repair Mix
- Control Reaction Protocol for PreCR Repair Mix
- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- Determining Genome Targeting Efficiency using T7 Endonuclease I
- Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (#M0386)
Application Notes for Nucleases
- A Fast One-Step Digestion of DNA or RNA for Global Detection and Characterization of Nucleotide Modifications
- AGBT 2018 Wellcome Sanger Institute poster: Thurston. S., et al. (2018). A Large Genome Centre Core Pipeline Refresh.
- Enzymatic removal of N- and O-glycans using PNGase F or the Protein Deglycosylation Mix
- Using Exonuclease V (RecBCD) to Eliminate Residual Genomic DNA When Purifying Low Copy Plasmids with the Monarch® Plasmid Miniprep Kit
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DNA Damage - the major cause of missing pieces from the DNA puzzle
- Properties of DNA Repair Enzymes
- DNA Damage and PreCR
- Traditional Cloning Quick Guide
Other Tools & Resources
Feature Articles
Selection Tools
Usage Guidelines
Common Applications of Exonucleases and Endonucleases
Properties of Exonucleases and Endonucleases
