Exonucleases and Non-specific Endonucleases
Exonucleases catalyze the removal of nucleotides in either the 5´ to 3´ or the 3´ to 5´ direction from single- or double-stranded DNA. These enzymes can be used in a wide variety of applications, including next-generation sequencing (NGS), PCR and gene synthesis.
Non-specific endonucleases cleave DNA and are generally used for DNA contamination removal. (To learn about DNA Repair Enzymes and Structure-specific endonucleases, visit here.)
Helping you select the right exonucleases and non-specific endonucleases
NEB offers a comprehensive selection of exonucleases and non-specific endonucleases for the cleavage of DNA, all of which have been optimized for robust performance in streamlined workflows.
Additional tools and resources
The following resources will help you select and learn more about exonucleases and non-specific endonucleases from NEB.
The effect of chemical modifications in nucleic acids on digestion by exonucleases (coming soon)
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Protocols for Exonucleases and Non-specific Endonucleases
- Protocol for T5 Exonuclease (M0363)
- Removal of Single-Stranded Extension Protocol using Mung Bean Nuclease (M0250)
- A Typical Exonuclease V Reaction (M0345)
- A Typical DNase I Reaction Protocol (M0303)
- T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- Standard Reaction Protocol for PreCR Repair Mix
- Sequential Reaction Protocol for PreCR Repair Mix
- Control Reaction Protocol for PreCR Repair Mix
- In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386)
- Determining Genome Targeting Efficiency using T7 Endonuclease I
- Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (#M0386)
Application Notes for Exonucleases and Non-specific Endonucleases
Legal Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
