Exonucleases and Non-specific Endonucleases
Exonucleases catalyze the removal of nucleotides in either the 5´ to 3´ or the 3´ to 5´ direction from single- or double-stranded DNA. These enzymes can be used in a wide variety of applications, including next-generation sequencing (NGS), PCR and gene synthesis.
Non-specific endonucleases cleave DNA and are generally used for DNA contamination removal. (To learn about DNA Repair Enzymes and Structure-specific endonucleases, visit here.)
Helping you select the right exonucleases and non-specific endonucleases
NEB offers a comprehensive selection of exonucleases and non-specific endonucleases for the cleavage of DNA, all of which have been optimized for robust performance in streamlined workflows.
Additional tools and resources
The following resources will help you select and learn more about exonucleases and non-specific endonucleases from NEB.
Choose Type:
- A Typical DNase I Reaction Protocol (M0303)
- A Typical Exonuclease V Reaction (M0345)
- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- Control Reaction Protocol for PreCR Repair Mix
- Removal of Single-Stranded Extension Protocol using Mung Bean Nuclease (M0250)
- Sequential Reaction Protocol for PreCR Repair Mix
- Standard Reaction Protocol for PreCR Repair Mix
- Protocol for T5 Exonuclease (M0363)
- In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386)
- Determining Genome Targeting Efficiency using T7 Endonuclease I
- Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (#M0386)
- T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
- Exo Selector
- Activities of Exonucleases and Non-specific Endonucleases
- Common Applications for Exonucleases and Endonucleases
- Properties of Exonucleases and Non-specific Endonucleases
Web Tools
Selection Tools
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Videos
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What are exonucleases and their applications?
Learn about exonucleases and some of their common applications.
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NEB: Exonucleases and Endonucleases as Molecular Tools Webinar
This webinar reviews the activities of exonucleases and endonucleases, provides insight on how to choose the right exo- or endonuclease for various molecular biology applications, and explains how to use these reagents when developing new molecular biology workflows.
