NEB offers a number of endonucleases and exonucleases that cleave DNA.
Exonucleases catalyze the removal of nucleotides in either the 5’ to 3’ or the 3’ to 5’ direction from single or double stranded DNA. Endonucleases cleave DNA nonspecifically and can be used to generate blunt ends for ligation or to shorten duplex DNA.
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The NEB PCR Cloning Kit allows direct cloning from any amplification reaction, with no purification needed!
The Gibson Assembly™ Cloning Kit is a robust method for assembling 1-2 DNA fragments, with a cloning vector, and ensuring high transformation efficiencies - all in a little less than 2 hours.
