Exonucleases catalyze the removal of nucleotides in either the 5´ to 3´ or the 3´ to 5´ direction from single- or double-stranded DNA. These enzymes can be used in a wide variety of applications, including next-generation sequencing (NGS), PCR and gene synthesis.
Non-specific endonucleases cleave DNA and are generally used for DNA contamination removal. (To learn about DNA Repair Enzymes and Structure-specific endonucleases, visit here.)
- A Typical DNase I Reaction Protocol (M0303)
- A Typical Exonuclease V Reaction (M0345)
- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- Control Reaction Protocol for PreCR Repair Mix
- Removal of Single-Stranded Extension Protocol using Mung Bean Nuclease (M0250)
- Sequential Reaction Protocol for PreCR Repair Mix
- Standard Reaction Protocol for PreCR Repair Mix
- Protocol for T5 Exonuclease (M0363)
- In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386)
- Determining Genome Targeting Efficiency using T7 Endonuclease I
- Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (#M0386)
- T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
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Learn about exonucleases and some of their common applications.
This webinar reviews the activities of exonucleases and endonucleases, provides insight on how to choose the right exo- or endonuclease for various molecular biology applications, and explains how to use these reagents when developing new molecular biology workflows.