NEB offers a large selection of fluorescent labels (substrates) for CLIP-fusion proteins. CLIP-tag™ substrates consist of a fluorophore conjugated to a cytosine leaving group via a benzyl linker. Substrates label the CLIP-tag without the need for additional enzymes. Cell-permeable substrates (CLIP-Cell™) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (CLIP-Surface™) are specific for fusion proteins expressed on the cell surface only.
CLIP-tag™, CLIP-Cell™ and CLIP-Surface™ are trademarks of New England Biolabs, Inc.
FAQs for CLIP-tag™ Substrates
Protocols for CLIP-tag™ Substrates
- Cellular Labeling (S9233)
- Cellular Labeling (S9219)
- Cellular Labeling (S9232)
- Cellular Labeling (S9217)
- Cellular Labeling (S9234)
- Labeling of Proteins in vitro (S9217)
- Labeling of Proteins in vitro (S9219)
- Labeling of Proteins in vitro (S9234)
- Labeling of Proteins in vitro (S9233)
- Labeling of Proteins in vitro (S9232)
- Use with CLIP-tag substrates (S9220)
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9236)
- Labeling of Proteins in vitro (S9220)
- Labeling of Proteins in vitro (S9221)
- Cellular Labeling (S9221)
- Reaction Conditions for Chemical Coupling (S9236)
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
SNAP-tag® Technologies: Novel Tools to Study Protein Function
Cellular Imaging & Analysis Brochure
The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
Other Tools & Resources
- Schulz C. and Köhn M. 2008. Simultaneous protein tagging in two colors Chemistry & Biology . 15, PubMedID: 18291310, DOI:
- Gautier A. et al. 2008. An engineered protein tag for multiprotein labeling in living cells Chemistry & Biology . 15, PubMedID: 18291317, DOI:
- Gautier A. et al. 2009. Selective cross-linking of interacting proteins using self-labeling tags J. Am. Chem. Soc. . 131, PubMedID: 19916541, DOI:
Publications related to CLIP-tag™ Substrates
SNAP-tag®, CLIP-tag™ and ACP/MCP-tag Substrate Selection Chart
Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.