CLIP-tag™ Substrates

Cellular Analysis

NEB offers a large selection of fluorescent labels (substrates) for CLIP-fusion proteins. CLIP-tag™ substrates consist of a fluorophore conjugated to a cytosine leaving group via a benzyl linker. Substrates label the CLIP-tag without the need for additional enzymes. Cell-permeable substrates (CLIP-Cell™) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (CLIP-Surface™) are specific for fusion proteins expressed on the cell surface only.

CLIP-tag™, CLIP-Cell™ and CLIP-Surface™ are trademarks of New England Biolabs, Inc.

  1. Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

    Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.

  2. SNAP-tag Overview Tutorial

    View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.

Featured Products

    Publications related to CLIP-tag™ Substrates:

  1. Gautier A. et al. (2009). Selective cross-linking of interacting proteins using self-labeling tags J. Am. Chem. Soc. . 131, 17954-17962. PubMedID: 19916541
  2. Gautier A. et al. (2008). An engineered protein tag for multiprotein labeling in living cells Chemistry & Biology . 15, 128-136. PubMedID: 18291317
  3. Schulz C. and Köhn M. (2008). Simultaneous protein tagging in two colors Chemistry & Biology . 15, PubMedID: 18291310