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  • NEBuffer Set 1.1, 2.1, 3.1 and CutSmart

    Description

    New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.

    NEBuffer 1.1
    1X Buffer Components

    10mM Bis-Tris-Propane-HCl
    10mM MgCl2
    100μg/ml BSA
    pH 7.0@25°C

    NEBuffer 2.1
    1X Buffer Components

    50mM NaCl
    10mM Tris-HCl
    10mM MgCl2
    100μg/ml BSA
    pH 7.9@25°C

    NEBuffer 3.1
    1X Buffer Components

    100mM NaCl
    50mM Tris-HCl
    10mM MgCl2
    100μg/ml BSA
    pH 7.9@25°C

    CutSmart Buffer
    1X Buffer Components

    50mM Potassium Acetate
    20mM Tris-acetate
    10mM Magnesium Acetate
    100μg/ml BSA
    pH 7.9@25°C

    Kit Components

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    NEBuffer 1.1-2010X
    NEBuffer 2.1-2010X
    NEBuffer 3.1-2010X
    CutSmart® Buffer-2010X

    Properties and Usage

    Storage Temperature

    -20°C

    Notes

    1. pH range: The pH of 10X NEBuffer 1.1 is between pH 6.9 and 7.1. The pH of 10X NEBuffer 2.1, NEBuffer 3.1 and CutSmart Buffer is between pH 7.8 and 8.0.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Endonuclease Activity (Nicking):
      The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • Functional Test (Restriction Digest, Buffer):
      The buffer is tested for function by performing a restriction enzyme activity assay.
    • Non-Specific DNase Activity (16 hour):
      The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
    • RNase Activity (16 Hour Digestion):

      The product is tested in a reaction containing a RNA substrate.  After incubation for 16 hours there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.