NEBuffer Set 1.1, 2.1, 3.1 and CutSmart

Description

New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.

NEBuffer 1.1
1X Buffer Components

10mM Bis-Tris-Propane-HCl
10mM MgCl2
100μg/ml BSA
pH 7.0@25°C

NEBuffer 2.1
1X Buffer Components

50mM NaCl
10mM Tris-HCl
10mM MgCl2
100μg/ml BSA
pH 7.9@25°C

NEBuffer 3.1
1X Buffer Components

100mM NaCl
50mM Tris-HCl
10mM MgCl2
100μg/ml BSA
pH 7.9@25°C

CutSmart Buffer
1X Buffer Components

50mM Potassium Acetate
20mM Tris-acetate
10mM Magnesium Acetate
100μg/ml BSA
pH 7.9@25°C

Kit Components

The following reagents are supplied with this product:

Store at (°C)Concentration
NEBuffer 1.1-2010X
NEBuffer 2.1-2010X
NEBuffer 3.1-2010X
CutSmart® Buffer-2010X

Properties and Usage

Storage Temperature

-20°C

Notes

  1. pH range: The pH of 10X NEBuffer 1.1 is between pH 6.9 and 7.1. The pH of 10X NEBuffer 2.1, NEBuffer 3.1 and CutSmart Buffer is between pH 7.8 and 8.0.

FAQs

  1. Why do I see additional DNA bands on my gel after a restriction digest?
  2. Why is my Restriction Enzyme not cutting DNA?
  3. Why do I see a DNA smear on an agarose gel after a restriction digest?
  4. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • Functional Test (Restriction Digest, Buffer):
    The buffer is tested for function by performing a restriction enzyme activity assay.
  • Non-Specific DNase Activity (16 hour):
    The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
  • RNase Activity (16 Hour Digestion):

    The product is tested in a reaction containing a RNA substrate.  After incubation for 16 hours there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.