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  • 7-deaza-dGTP

    Description

    7-deaza-dGTP (7-deaza-2´-deoxyguanosine 5´-triphosphate) contains a 5 mM solution of 7-deaza-dGTP as a dilithium salt. Nucleotide concentration is determined by measurements of absorbance at 257 nm.

    Supplied in: Milli-Q® water as a lithium salt at (pH 7.0).

    Properties and Usage

    Storage Temperature

    -20°C

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Non-Specific DNase Activity (16 hour):
      The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
    • PCR Amplification (dNTPs):
      The dNTPs are tested in a polymerase chain reaction (PCR) using Taq DNA Polymerase, a control template and specific primers, resulting in the expected product.
    • Phosphatase Activity (PNPP):
      The product is tested in a reaction containing a p-nitrophenyl phosphate (PNPP), a chromogenic substrate for most phosphatases. After incubation there is no detectable phosphatase activity as determined by spectrophotometric analysis of released p-nitrophenol at 405 nm.
    • Physical Purity (HPLC):
      The purity of the product is determined by HPLC analysis.

    Notes

    1. Can be diluted using sterile distilled water, preferably Milli-Q water or can be diluted using sterile TE [10 mM Tris-HCl, 1 mM EDTA (pH 7.5)].

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What is 7-deaza-dGTP used for?
    2. How stable are the nucleotides?