NEBNext Direct® employs a unique technology that enables highly specific target enrichment of genomic regions of interest. The result of a partnership between New England Biolabs®(NEB®) and Directed Genomics™, this innovative approach to target enrichment balances the speed and precision of multiplexed PCR-based approaches with the content scalability typical of hybridization-based methods. This flexibility allows a single workflow for assays ranging from single gene tests to comprehensive panels including several hundred genes. Regardless of sample type or assay content, NEBNext Direct allows you to enrich your targets with precision.
Target enrichment offers several advantages over more comprehensive genomic profiling for a variety of scientific and applications. During this webinar, we explore the advantages and limitations of target enrichment, and discuss how NEBNext Direct®, a novel solution for selective enrichment of genomic targets, addresses these challenges.
View our tutorial for help visualizing NEBNext Direct’s unique and enabling workflow.
Precision medicine requires precision enrichment. Learn more about target enrichment and its role in clinical applications.
Learn about the innovative NEBNext Direct technology for target enrichment for next generation sequencing.
Now Available: The NEBNext Direct BRCA1/BRCA2 Panel
New white paper available: Overcoming the challenges of applying target enrichment for translational research
For more information, please contact NEBNextDirect@neb.com.
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- Generate a higher percentage of your sequencing reads aligning to your targets
- Obtain uniform sequencing of ALL targets, regardless of GC content
- Save time with a streamlined workflow that couples enrichment and library preparation
- Generate high quality libraries with limited input amounts and degraded DNA samples, including FFPE and ctDNA
- Distinguish molecular duplicates, reducing false positive variants and
- On-bead sample preparation is ideal for automated workflows
What People are Saying
NEBNext Direct enrichment technology is by far the fastest and most automation friendly protocol available today. I can have samples on the sequencer in 6 hours starting from genomic DNA. The technology produces very high on target percentages (>90%) for even very small panels, and in combination with molecular barcoding produces low duplication rates. From an optimization perspective, NEBNext Direct enrichment allows me to assign individual captured fragments to a probe unambiguously, thus giving the opportunity for optimizing the coverage distribution of any target.
Using the NEBNext Direct kit, we were able to detect all known single nucleotide variants and indels in DNA extracted from fresh frozen or FFPE tissue derived from glioma biopsies. We could also clearly see amplification in genes like EGFR or PDGFR. The workflow is really easy and fast and can be rapidly implemented in a lab.
The kit and its technology are easy to use and easy to automate, allowing us to get up and running quickly. The protocol itself is fast and efficient to obtain deep coverage of targets, giving homogeneous results for FFPE and frozen tumors, therefore opening doors for customized panels.
NEW ENGLAND BIOLABS® and NEB® are registered trademarks of New England Biolabs, Inc.
NEBNext Direct® is a trademark of New England Biolabs, Inc.
Directed Genomics™ is a trademark of Directed Genomics