NEBNext Direct® for target enrichment

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NEBNext Direct^® offers a novel approach to NGS Target Enrichment. Using a unique, capture-based protocol, NEBNext Direct offers a range of solutions for highly specific, efficient targeting of genomic loci of interest. Optimized, 1-day protocols allow for rapid profiling of targeted regions for a variety of applications, including germline and somatic variant calling for translational research and genotyping of genomic markers for applications in agricultural biology and sample identification.

Define your own targeted sequencing panel by selecting only the genes you want from our expansive list of genes using NEBNext Direct Custom Ready Panels. For genotyping applications, the NEBNext Genotyping Solution enables pre-capture pooling of up to 96 samples for cost-effective and scalable genotyping of up to 5,000 genomic markers.

Videos

Highly multiplexed capture of genomic markers for high throughput NGS based genotyping

In this ESHG 2020 workshop, hear about methylation analysis of cfDNA samples, sample identification and tracking using a high-throughput SNP genotyping method and the importance of sample prep in large-scale sequencing pipelines.
Fully Automated Targeted SNP Genotyping for Plant and Animal Applications

This webinar from NEB^®, EMBL and Beckman Coulter^® Life Sciences discusses the development and verification of the automated NEBNext Direct^® Genotyping Solution on the Beckman Coulter i7 workstation to achieve highly-multiplexed sample processing and meet the growing demands of plant and animal genotyping applications.
NEB^® TV Ep. 11 – Target Enrichment for NGS

Precision medicine requires precision enrichment. Learn more about target enrichment and its role in clinical applications.