コンピテントセル製品
クローニング用株
クローニング実験には、複数の高効率コンピテントセル株から選択する。これらの大腸菌株はT1ファージに耐性があり、endAが欠損しているため、高品質のプラスミド調製が可能である。さらに、全てのNEBのコンピテントセルは動物由来の物質を含まない。
酵母
NEBは化学的にコンピテントなKluyveromyces lactis細胞、および特定のタンパク質発現ニーズに合わせたこの株の変異体を提供している。これらの細胞は、当社の直鎖型pKLACシリーズ発現ベクターを用いた形質転換に適している。
タンパク質発現用大腸菌株製品
NEBは、さまざまなレベルで発現が制御され、それぞれがT1ファージ耐性と極めて高い形質転換効率を有する株を各種提供している。
NEBの高効率のコンピテントセル株ポートフォリオから、クローニングやタンパク質発現のアプリケーションに適した細胞を選択。
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クローニング用大腸菌コンピテントセル
発現用大腸菌株製品
酵母発現株製品
コンピテントセル用バッファーおよび希釈液製品
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- Can I store competent cells at -20°C instead of -80°C?
- Does New England Biolabs offer a methyltransferase free strain of Competent E.coli?
- Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
- What are the advantages of New England Biolabs' competent cells?
- What does the growth curve of NEB Turbo cells look like compared to DH5α?
- What is LysY?
- What volume of DNA can be added into competent cells?
- Which kind of transformation tubes should be used?
- What applications are SHuffle® strains useful for?
- Why did Synthetic Biologist Chris Voigt of MIT choose NEB 10-beta for DNA assembly and cloning?
- Which strain of Competent E.coli should I use for general cloning?
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Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
- コンピテントセル
- タンパク質発現＆精製
- Competent Cell Selection Guide
- Competent Cell Product Comparison
- Characteristics of Select E.coli Strains
- Troubleshooting Transformation Reactions
- Restriction of Foreign DNA by E. coli K-12
- Additional E. coli Strain Genotypes
- McrA, McrBC and EcoKI Strain Phenotypes
- Chemical Transformation Tips
- Competent E. coli Genome Sequences Tool
- Making Unmethylated (Dam- Dcm-) DNA
- Electroporation Tips
- Genetic Markers
Feature Articles
Brochures
Selection Tools
Troubleshooting Guides
Usage Guidelines
- Narayanan, A., Ridilla, M. and Yernool, D.A. (2010) Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator–repressor interactions Protein Sci; 20 , 51-61 .
- Schlegel, S., Klepsch, M., Gialama, D., Wickström, D., Slotboom, D.J. and de Gier, J. (2010) Revolutionizing membrane protein overexpression in bacteria Microb Biotechnol; 3 , 403-411 .
- Reddy, P.T., Brinson, R.G., Hoopes, J.T., McClung, C., Ke, N., Kashi, L. (2018) Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli. mAbs MAbs; 10 (7), 992-1002. PubMedID: 30060704, DOI: 10.1080/19420862.2018.1496879
- Agrawal, A., Bisharyan, Y., Papoyan, A, Bednenko, J., Cardarelli, J., Yao, M., Clark, T., Berkmen, M., Ke, N., Colussi, P. (2019) Fusion to Tetrahymena thermophila granule lattice protein 1 confers solubility to sexual stage malaria antigens in Escherichia coli. Protein Expr Purif; 153, 7-17. PubMedID: 30081196, DOI: 10.1016/j.pep.2018.08.001.
- Reuter, W.H., Masuch, T., Ke, N., Lenon, M., Radzinski, M., Van Loi, V., Ren, G., Riggs, P., Antelmann, H., Reichmann, D., Leichert, L.I., Berkmen, M (2019) Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote Redox Biol; 26, 101280. PubMedID: 31450103, DOI: 10.1016/j.redox.2019.101280
- Leith, E.M., O'Dell, W.B., Ke, N., McClung, C., Berkmen, M., Bergonzo, C., Brinson, R.G., Kelman, Z (2019) Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli J Biol Chem; 294(48), 18046-18056.. PubMedID: 31604819, DOI: 10.1074/jbc.RA119.011008
- Ren, G., Ke, N. and Berkmen, M. (2016) Use of the Shuffle Strains in Production of Proteins. Curr Protoc Protein Sci; Aug 1, 1;85:5.26.1-5.26.21.. PubMedID: 27479507 , DOI: 10.1002/cpps.11.
- Hibender, S. Landeta, C., Berkmen, M., Beckwith, J., Boyd, D. (2017) Aeropyrum pernix membrane protein VKOR promotes protein disulfide bond formation in two subcellular compartments. Microbiology; 163 (12), 1864-1869. PubMedID: 291309344
- Wagner, S., Klepsch, M., Schlegel, S., Appel, A., Draheim, R., Tarry, M., Hö, M., van Wijk, K.J., Slotboom, D.J., Persson, J.O. and de Gier, J. (2008) Tuning Escherichia coli for membrane protein overexpression Proc Natl Acad Sci U S A; 105 , 14371-14376 .
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