Home FAQs Can I use the NEBNext® High Input Poly(A) mRNA Isolation Module with total RNA inputs higher than 50 µg? What about lower inputs?

FAQ: Can I use the NEBNext® High Input Poly(A) mRNA Isolation Module with total RNA inputs higher than 50 µg? What about lower inputs?

For best results when using total RNA inputs higher than 50 µg, all volumes in the protocol can be proportionally scaled. For inputs less than 5 µg, the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) is recommended.