Home FAQs How can I increase methylation of my substrate by EcoGII Methyltransferase?

FAQ: How can I increase methylation of my substrate by EcoGII Methyltransferase?

SAM is unstable at pH 7.5, 37 °C. For extended reactions adding more SAM after 4 hours can improve results. Methylation reactions, however, are also greatly affected by S-adenosylhomocysteine. S-adenosylhomocysteine, the byproduct of the methylation reaction binds more tightly to methylases than does SAM. Inhibition by S-adenosylhomocysteine greatly reduces the reaction rate as time passes.

Please see the table on the product description page for more details on methylation levels of various substrates by EcoGII Methyltransferase.