New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.
HomeFAQsWhat are the best techniques to analyze N- and O-glycans?
FAQ: What are the best techniques to analyze N- and O-glycans?
Glycans are neutral or charged molecules with no intrinsic UV absorbance or autofluorescent emission. Therefore carbohydrate analysis requires dedicated instrumentation. Glycans released from proteins can be analyzed directly by HPAEC-PAD (High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection); or they are derivatized with fluorescent or methyl groups for subsequent analysis by capillary electrophoresis and/or liquid chromatography/mass spectrometry (LC/MS). Finally, abundant glycan samples can be separated by TLC, however the resolution is poor for complex mixtures and large molecules.
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