HomeFAQsWhat are the best techniques to analyze N- and O-glycans?
FAQ: What are the best techniques to analyze N- and O-glycans?
Glycans are neutral or charged molecules with no intrinsic UV absorbance or autofluorescent emission. Therefore carbohydrate analysis requires dedicated instrumentation. Glycans released from proteins can be analyzed directly by HPAEC-PAD (High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection); or they are derivatized with fluorescent or methyl groups for subsequent analysis by capillary electrophoresis and/or liquid chromatography/mass spectrometry (LC/MS). Finally, abundant glycan samples can be separated by TLC, however the resolution is poor for complex mixtures and large molecules.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.