HomeFAQsIs there a simple way to remove Trypsin after protein cleavage?
FAQ: Is there a simple way to remove Trypsin after protein cleavage?
Bezamidine Sepharose (pH 8) will remove intact Trypsin, although some of the desired peptides may be lost in the process. The best strategy is to keep the ratio of substrate to trypsin, high, e.g. 50-100:1.