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RNA Polymerase Selection Chart

T7 and SP6 RNA polymerases are DNA dependent RNA polymerases that produce DNA templated RNA transcripts. T7 and SP6 exhibit high specificity for their respective promoters. Both T7 and SP6 can be used for the in vitro synthesis of RNA for a wide variety of applications, including transfection, translation, structural studies and radioactive and non-isotopic probe generation. E. coli Poly(A) Polymerase and Poly(U) Polymerase generate untemplated homoribopolymeric tails on RNA 3´-ends. Both E. coli Poly(A) Polymerase and Poly(U) Polymerase can be used for RNA tailing prior to reverse transcription or for direct labeling.

Product	NEB #	Applications	Size
Product	NEB #	Applications	Size	T3 RNA Polymerase	M0378S	Radiolabeled RNA probes Non-isotopic RNA labeling Preparation of RNA vaccines Guide RNA for gene targeting mRNA for in vitro translation and micro injection RNA structure, processing and catalysis studies RNA amplification Anti-sense RNA for gene expression experiment	5000 units
T7 RNA Polymerase	M0251S M0251L	Radiolabeled RNA probes Non-isotopic RNA labeling Preparation of RNA vaccines Guide RNA for gene targeting mRNA for in vitro translation and micro injection RNA structure, processing and catalysis studies RNA amplification Anti-sense RNA for gene expression experiment	5000 units 25000 units
Hi-T7^® RNA Polymerase	M0658S	Radiolabeled RNA probes Non-isotopic RNA labeling Guide RNA for gene targeting mRNA for in vitro translation, microinjection and transfection RNA structure, processing and catalysis studies RNA amplification Isothermal amplification Anti-sense RNA for gene expression	5000 units
SP6 RNA Polymerase	M0207S	Radiolabeled RNA probe preparation Non-isotopic RNA labeling Preparation for RNA vaccines Guide RNA for gene targeting mRNA for in vitro translation and micro injection RNA structure, processing and catalysis studies RNA amplification Anti-sense RNA for gene expression experiment	2000 units
E. coli Poly(A) Polymerase	M0276S M0276L	Labeling of RNA with ATP or cordycepin Poly(A) tailing of RNA for cloning or affinity purification Enhances translation of RNA transferred into eukaryotic cells.	100 units 500 units
Poly(U) Polymerase	M0337S	Labeling of RNA with UTP Poly(U) and Poly(A) tailing of RNA for cloning Studying effects of poly(U) tailing on stability and translation of RNA transferred into eukaryotic cells Poly (A) tailing of 2' O-Me modified 3' ends	60 units
E. coli RNA Polymerase, Core Enzyme	M0550S	RNA synthesis from E. coli promoter Transcription initiation studies In vitro translation with PURExpress	100 units
E. coli RNA Polymerase, Holoenzyme	M0551S	RNA synthesis from E. coli promoter Transcription initiation studies In vitro translation with PURExpress	50 units

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