microRNA Marker

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
N2102S     -20       microRNA Marker N2102SVIAL -20 1 x 0.5 ml 12 ng/µl   MicroRNA Marker Probe (21-mer) S1328SVIAL -20 1 x 0.5 ml 20 ng/µl

Companion Products

• Low Range ssRNA Ladder
• T4 Polynucleotide Kinase

1. The microRNA Marker is provided in a ready-to-load denaturing solution. Denature by heating for 3-5 minutes at 95°C and place on ice. Load 5-10 µl for staining with SYBR Gold in denaturing gels. In Northern blots, less than 1 µl (12 ng) is sufficient for detection by hybridization. 
   
   The Orange G loading buffer migrates faster than the smallest band, and migrates approximately as far as the nucleotides.
2. Oligonucleotide Probe Usage: The provided biotinylated probe DNA oligonucleotide can be labeled with T4 Polynucleotide Kinase (NEB #M0201) and radioactive γ-³²P-ATP using the following protocol: 
   
   1. Mix the following components in a sterile microfuge tube:
   
      Oligonucleotide Probe -- 1-5 µl
      10X T4 Polynucleotide Kinase reaction Buffer -- 2.0 µl
      γ-³²P-ATP (5 µCi/µl) -- 1-2 µl
      T4 Polynuclotide Kinase -- 1 µl
      Sterile dH₂0 -- X µl
      Total volume -- 20 µl 
   
   2. Incubate for 30 minutes at 37°C. 
   
   3. Purify labeled probe using a G-25 spin column.

1. Sambrook, J. and Russel, D.W. (2001). Molecular Cloning: A Laboratory Manual (3rd Ed.). 7.1-7.56.

1. Can I use the microRNA Marker on a non-denaturing gel?
2. Should I denature the microRNA Marker before loading on my gel?
3. What is the difference between the siRNA marker and the microRNA marker (NEB #N2101S)?
4. Can I use EtBr for staining the microRNA Marker after gel electrophoresis?
5. Should I load more of the microRNA Marker so I can get better visibility of the bands after staining the gel with EtBr?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• N2102S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• N2102S_v0_10016079
• N2102S_v0_10030305
• N2102S_v1_10052797
• N2102S_v1_10056895
• N2102S_v1_10060000
• N2102S_v1_10064745
• N2102S_v1_10082926
• N2102S_v1_10100257
• N2102S_v1_10115305
• N2102S_v1_10151119
• N2102S_v1_10167481
• N2102S_v1_10180893
• N2102S_v1_10198998

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• microRNA Marker

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.