Home Markers & Ladders Products Quick-Load^® Purple 1 kb Plus DNA Ladder

Quick-Load^® Purple 1 kb Plus DNA Ladder

This product was previously named the Quick-Load^® Purple 2-Log DNA Ladder. There have been no changes to product formulation or specifications.

Convenient, ready-to-load format with NEB's proprietary purple loading dye
Size range: 100 bp to 10 kb (100 bp and 1 kb ladder in one!)
Sharp bands
No UV Shadow
Easy to identify reference bands
Supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
Small size suitable for 250 gel lanes; large size suitable for 750 gel lanes

Catalog #	Concentration	Size	List Price	Your Price
N0550S	100 µg/ml	250 gel lanes (1.25 ml)	$82.00	Sign In Sign In
N0550L	100 µg/ml	750 gel lanes (3.75 ml)	$206.00	Sign In Sign In

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Product Information

Quick-Load Purple 1 kb Plus DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. Tracking dye does not leave a shadow under UV light.

Source
Quick-Load Purple 1 kb Plus DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.

Recommended gel percentage range: 1-1.4%
Optimum separation on 1.2%

This product is related to the following categories:: Quick-Load Purple DNA Ladders Products,; DNA Markers & Ladders Products

This product can be used in the following applications:: DNA Analysis

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

N0550S 4

Quick-Load^® Purple 1 kb Plus DNA Ladder N0550SVIAL 4 1 x 1.25 ml 100 µg/ml

Gel Loading Dye, Purple (6X), no SDS B7025SVIAL 25 1 x 1 ml 6 X

Properties & Usage

Bases

Fragment	Mass (ng)	bp
1	40	10,002
2	40	8,001
3	48	6,001
4	40	5,001
5	32	4,001
6	120	3,001
7	40	2,017
8	57	1,517
9	45	1,200
10	122	1,000
11	34	900
12	31	800
13	27	700
14	23	600
15	124	500/517
16	49	400
17	37	300
18	32	200
19	61	100

Effective Size Range

100bp to 10,002bp

Storage Buffer

2.5% Ficoll®-400
10 mM EDTA
3.3 mM Tris-HCl
0.001% Dye 2
0.02% Dye 1
pH 8 @ 25°C

Related Products
Companion Products
Product Notes
1. Preparation of DNA
  The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
  
  Usage recommendation
  We recommend loading 5-10 ul (0.5-1 ug) of Quick-Load Purple 1 kb Plus DNA Ladder per gel lane. The Quick-Load Purple 1 kb Plus DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
2. Quick-Load Purple 1 kb Plus DNA Ladder is stable for at least 6 months at 25°C.
  For long term storage, store at 4°C or -20°C.
  If stored at -20°C, mix well after thawing. If not well mixed, the load may float out of the wells.
References
1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular cloning: A Laboratory Manual. (2nd ed.), (pp. 10.51-10.67). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

Protocols, Manuals & Usage
- Protocols
  Protocol for Quick-Load Purple 1kb Plus DNA Ladder (N0550)
Tools & Resources
- Selection Charts
  DNA Markers & Ladders Selection Tool
FAQs & Troubleshooting
- FAQs
  Why are there extra bands visible on polyacrylamide gels?
  
  Can I use Midori Green with the DNA Ladders from NEB?
  
  Can I use SYBR^® and/or GelRed^® dyes with the DNA Ladders from NEB?
  
  Why is my DNA Ladder floating out the wells and Why are there no bands visible in my DNA ladder gel lane?
  
  What are the differences in the four versions of 2-Log, 100 bp and 1 kb DNA Ladders that NEB sells?
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  N0550S_v1
  
  N0550S_L_v2
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  N0550S_v1_0031407
  
  N0550S_v1_0021401
  
  N0550S_v1_0031406
  
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  N0550G_v1_10251325
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Quick-Load^® Purple 1 kb Plus DNA Ladder
  
  Gel Loading Dye, Purple (6X), no SDS
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Quick-Load^® Purple 1 kb Plus DNA Ladder