Exonuclease T
NEB4 cloned at NEB recombinant 25 65 Heat

  • Also known as RNase T
  • Single-stranded (ssDNA or RNA) specific exonuclease
  • Catalyzes the removal of nucleotides from linear single-stranded DNA or RNA in the 3' to 5' direction
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Exonuclease T can be used for:

  • Removal of 3' overhangs of double-stranded DNA to generate blunt-ends*
  • Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
  • Removal of single-stranded primers for nested PCR reactions
  • Removal of single-stranded DNA, leaving behind double-stranded DNA in the sample
  • Not sure which RNase to choose? Learn more about the different RNases offered by NEB.


Catalog # Concentration Size List Price Your Price Quantity
M0265S 5,000 units/ml 250 units $82.00
M0265L 5,000 units/ml 1,250 units $333.00
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