- Can cell lysates be used in the PCR reaction?
- My PCR reaction yield is low, can I add more than 5 µl of the PCR reaction to the digestion reaction?
- What are the expected results using the included control?
- Why do I see an extra band when I run the undigested heteroduplex on an agarose gel?
- Why do I see very little DNA on my gel or low signal on the fragment analyzer?
- Will EnGen® T7 Endonuclease I recognize single base mismatches and single base insertions or deletions (indels)?
- T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
- Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (#M0386)
- Transfection of EnGen® Spy Cas9 HF1 (NEB #M0667) into adherent cells using the Lipofectamine® RNAiMAX System
CRISPR/Cas9 & Targeted Genome Editing: New Era in Molecular Biology
Understand the history, importance and future of CRISPR/Cas9 and target genome editing
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Get a high level overview of CRISPR/Cas9 and how it is used in genome editing in our Science in 60 segment.