Luna® Universal qPCR & RT-qPCR
Optimize One-Step RT-qPCR with unique WarmStart® technology
The Luna One-Step RT-qPCR kits contain a novel, in silico-designed reverse transcriptase (RT) engineered for improved performance. Both the Luna WarmStart Reverse Transcriptase and Hot Start Taq DNA Polymerase, included in these kits, utilize a temperature-sensitive, reversible aptamer, which inhibits activity below 45°C. This enables room temperature reaction setup and prevents undesired non-specific activity. Furthermore, the WarmStart RT has increased thermostability, improving performance at higher reaction temperatures.
Improve performance with our novel, thermostable reverse transcriptase
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Luna WarmStart Reverse Transcriptase exhibits exceptional sensitivity, reproducibility and RT-qPCR performance
RT-qPCR targeting human GAPDH was performed using the Luna Universal One-Step RT-qPCR Kit, featuring Luna WarmStart Reverse Transcriptase, over an 8-log range of input template concentrations (0.1 pg – 1 μg Jurkat total RNA) with 8 replicates at each concentration. NTC = non-template control.
Achieve better control and better results: WarmStart Luna RT paired with Hot Start Taq
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WarmStart Luna RT prevents spurious amplification resulting from room-temperature pre-incubation
RT-qPCR targeting human ribosomal protein L32 was performed before and after a 24-hour incubation at room temperature, with triplicate reactions for a 5-log range of input human (Jurkat) total RNA and a non-template control. The Luna Universal One-Step RT-qPCR Kit featuring Luna WarmStart Reverse Transcriptase exhibited robust performance and no detectible non-template amplification, either with or without a 24 hour 25°C pre-incubation, while the ABI 1-Step Kit, featuring a non-WarmStart reverse transcriptase, exhibited significant non-template amplification.
Greater thermostability means greater versatility
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The increased thermostability of WarmStart Luna Reverse Transcriptase improves performance at increased reaction temperatures
RT-qPCR experiments targeting human ribosomal protein L32 RNA were performed in triplicate over a 5-log range of input human (Jurkat) total RNA (5 pg – 50 ng) using an initial 10 min RT step performed at 50°C – 60°C, as indicated.
A. Luna WarmStart Reverse Transcriptase (recommended incubation temperature: 55°C) exhibited rapid Cq values (bar graph) and robust RT-qPCR performance (amplification plots) at each temperature, indicating that efficient reverse transcription was not perturbed by reaction temperature alterations.
B. In contrast, a commercially available MMLV (recommended incubation temperature: 50°C) exhibited delayed (increased) Cq values, poorer performance, and loss of low-input detection at elevated temperatures, consistent with loss of RT activity.
Available products:
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Luna Universal qPCR Master Mix Rapid, sensitive, and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences |
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Luna Universal Probe qPCR Master Mix Rapid, sensitive, and precise probe-based qPCR detection and quantitation of target DNA and cDNA sequences |
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Luna Universal One-Step RT-qPCR Kit Includes everything you need for rapid, sensitive, and precise dye-based qPCR detection and quantitation of RNA targets |
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Luna Universal Probe One-Step RT-qPCR Kit Includes everything you need for rapid, sensitive, and precise probe-based qPCR detection and quantitation of RNA targets |
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Luna Probe One-Step RT-qPCR 4X Mix with UDG 4X mix enables sensitive detection of target RNA sequences, with robust performance in multiplex applications of up to 5 targets |
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LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG Lyophilized format enables sensitive detection of target RNA sequences with room temperature stability |
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Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit For real-time qualitative detection of SARS-CoV-2 nucleic acid using hydrolysis probes |
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LunaScript RT SuperMix Kit Single tube with blue tracking dye includes everything needed for first strand cDNA synthesis in two-step RT-qPCR workflows |
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LunaScript RT Master Mix Kit (Primer-free) Single-tube 5X master mix contains Luna Reverse Transcriptase, dNTPs, and Murine RNase Inhibitor |
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Luna Cell Ready One-Step RT-qPCR Kit All the necessary components for direct, dye-based RNA detection and quantitation from cell lysate, bypassing the need for RNA extraction and purification |
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Luna Cell Ready Probe One-Step RT-qPCR Kit All the necessary components for direct, probe-based RNA detection and quantitation from cell lysate, bypassing the need for RNA extraction and purification |
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Luna Cell Ready Lysis Module Coordinates cell lysis, RNA release, and genomic DNA removal simultaneously, resulting in cell lysate ready for direct RT-qPCR analysis with Luna Universal One-Step RT-qPCR kits |
