HomeFAQsWhat PCR reagents are recommended for DNA amplification in genome editing (CRISPR/Cas9, TALEN, ZFN) mismatch detection assays?
FAQ: What PCR reagents are recommended for DNA amplification in genome editing (CRISPR/Cas9, TALEN, ZFN) mismatch detection assays?
NEB recommends Q5® Hot Start High-Fidelity 2x Master Mix (NEB #M0494). Q5 DNA Polymerases offer high fidelity and robustness. For some highly repetitive regions, LongAmp® Taq DNA Polymerase (NEB #M0323) has worked well.
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