Argonautes
The list of known prokaryotic argonautes is continually growing as the field expands. Some examples of DNA:DNA argonautes (meaning DNA-guided DNA-endonuclease) include those from Pyrococcus furiosus (PfAgo), Thermus thermophilus (TtAgo), and Methanocaldococcus jannaschii (MjAgo). RNA:DNA argonautes include those from Marinitoga piezophila (MpAgo), Thermotoga profunda (TpAgo), and Rhodobacter sphaeroides (RsAgo). DNA:RNA argonautes include those from Aquifex aeolicus (AaAgo) and (with reduced activity) Thermus thermophilus (TtAgo). Finally, RNA:RNA argonautes include those from Marinitoga piezophila (MpAgo), and for comparison, eukaryotic argonaute human (AGO2).
NEB now offers the first commercially available argonaute Tth Argonaute (TtAgo) (NEB #M0665), a DNA-guided DNA-endonuclease for in vitro applications.
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- GUIDElines for optimization of Tth Argonaute (TtAgo) reactions
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