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Low input NEBNext Ultra II RNA libraries retain superior complexity
Nondir_RNA_Complexity_Figure1
Poly(A)-containing mRNA was isolated from Human Universal Reference RNA (Agilent #740000), and libraries were prepared using the NEBNext Ultra II RNA Kit (plus the NEBNext Poly(A) mRNA Magnetic Isolation Module), and the Illumina TruSeq RNA Sample Preparation Kit v2. Salmon 0.4.0 was used for read mapping and quantification of all GENCODE v25 transcripts. TPM = Transcript Per Kilobase Million. R2 values for the linear fit are shown. For each set, the lower figures show an expanded view of the 0–1,000 TPM range. Correlation analysis of the transcripts indicates very good transcript expression correlation between the different input amounts.

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NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) for sequencing on the Illumina platform.
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NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) plus SPRIselect® beads for size selection and cleanup.