HomeFAQsCan I use a different buffer for elution instead of nuclease-free water when using the Monarch® Spin Columns S3B (NEB #T2067)?
FAQ: Can I use a different buffer for elution instead of nuclease-free water when using the Monarch® Spin Columns S3B (NEB #T2067)?
Elution with nuclease-free water is standard, but for samples that will be stored for use later, EDTA can be added to 0.1–1.0 mM to limit degradation due to magnesium-requiring nucleases. Alternatively, elution with slightly alkaline TE can be employed.
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