Depending on the targets/primers, the optimal cDNA input may vary and should be optimized but typically, we recommend 1 μl of the cDNA reaction as input into a 25 μl PCR. Where useful, up to 20% PCR volume can be added (e.g. 5 μl cDNA product in a 25 μl PCR).
FAQ: When using LunaScript cDNA products in downstream PCR (i.e., two-step RT-PCR), what volumes and PCR products can be used?
Depending on the targets/primers, the optimal cDNA input may vary and should be optimized but typically, we recommend 1 μl of the cDNA reaction as input into a 25 μl PCR. Where useful, up to 20% PCR volume can be added (e.g. 5 μl cDNA product in a 25 μl PCR).
