Hi-T4 DNA Ligase is 100% active in T4 DNA Ligase Buffer.
Like T4 DNA Ligase, Hi-T4 DNA Ligase has reduced activity in NEBuffer 3/r3.1 and HiFi Taq DNA Ligase Buffer due to their high salt content.
|
Buffer |
% Activity1 |
|---|---|
|
T4 DNA Ligase Buffer |
100% |
|
NEBuffer r1.12 |
100% |
|
NEBuffer r2.12 |
100% |
|
NEBuffer r3.12 |
50% |
|
rCutSmart® Buffer2 |
100% |
|
Taq DNA Ligase Buffer2 |
100% |
|
HiFi Taq DNA Ligase Buffer2 |
50% |
1Activity relative to activity on a cohesive end substrate in T4 DNA Ligase Buffer (25°C for 10 minutes).
2 Supplemented with 1 mM ATP.
Please be sure to use riboATP (NEB #P0756) as deoxyriboATP will not work. To see its % functional activity in CutSmart, and that of other DNA modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in CutSmart® Buffer chart.
