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T4 DNA Ligase Products Table

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.

This enzyme will join blunt-end and cohesive-end termini, as well as repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

T4 DNA Ligase is available in a variety of formulations and variants.

The table below lists products available from NEB.

Product NEB # Features Reaction Conditions

T4 DNA Ligase

Regular concentration:

M0202S/L

High (5X) concentration:

M0202T/M  

  • Standalone enzyme ideal for a variety of ligation reactions
  • Ligation can be performed in supplied buffer, or in any of the four restriction endonuclease NEBuffers, or in T4 Polynucleotide Kinase Buffer if supplemented with 1 mM ATP
 1X T4 DNA Ligase Reaction Buffer. Incubate at 16°C. Heat inactivate at 65°C for 10 minutes.
Master Mixes
Instant Sticky-end Ligase Master Mix

M0370 

  • Ready-to-use 2X solution of T4 DNA Ligase and a proprietary ligation enhancer in an optimized reaction buffer.
  • Specifically formulated to rapidly ligate cohesive-end (2–4 bp) substrates and improve transformation
 1X Instant Sticky-end Ligase Master Mix with DNA substrates in a 10 µl reaction volume.
Blunt/TA Ligase Master Mix

M0367

  • Ready-to-use 2X solution of T4 DNA Ligase and a proprietary ligation enhancer in an optimized reaction buffer
  • Specifically formulated to improve ligation and transformation of both blunt-end and single-base overhand substrates
 1X Blunt/TA Ligase Master Mix with DNA substrates in a 10 µl reaction volume. Incubate at 25°C.
NEBridge® Ligase Master Mix

M1100
  • Ideal for high-efficiency and high-fidelity Golden Gate Assembly with a broad assortment of Type IIS restriction enzymes
  • Available in 3X master mix format
 1X NEBridge Ligase Master Mix, Type IIS restriction enzyme and DNA fragments in a 15 or 30 µl reaction volume. Follow guidelines.
Formulations
Quick Ligation™ Kit

M2200

  • Ligation of cohesive- or blunt-end DNA fragments in 5 minutes at room temperature (25°C)
 1X Quick Ligation Reaction Buffer. Incubate at room temperature (25°C).
ElectroLigase® M0369
  • Combines T4 DNA Ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG
  • Promotes robust ligation of all types of DNA ends (blunt, sticky, TA) and is suitable for electroporation, without desalting or purification
 1X ElectroLigase Reaction Buffer with DNA substrates and 1 µl ElectroLigase in an 11 µl reaction volume, incubated at 25°C.
Immobilized T4 DNA Ligase M0569
  • Enzyme is covalently linked to a magnetic bead, and can be removed from a reaction and reused
  • Enables ligated product to be used directly with no heat inactivation step
 1X T4 DNA Ligase Reaction Buffer. Incubate at 25°C. Place on magnet for 3 minutes to remove.
Master Mixes
Hi-T4™ DNA Ligase

M2622 

  • Enables ligation with improved thermostability
  • Active in temperatures up to 50°C
 1X T4 DNA Ligase Reaction Buffer. Incubate at 25°C. Heat inactivate at 65°C for 10 minutes.
Salt-T4® DNA Ligase

M0467 

  • Enables ligation with improved salt tolerance
  • Active in reactions as high as 300 mM salt with no loss in activity
 1X T4 DNA Ligase Reaction Buffer at 25–50°C. Heat inactivate at 65°C for 10 minutes


T4 DNA Ligase (NEB #M0202) is available as a GMP-grade reagent.