Protease Selection Chart
NEB offers several proteases with different recognition sites.
| Product | NEB # | Recognition Site |
|---|---|---|
| Factor Xa Protease | P8010 | Ile-Glu/Asp-Gly-Arg▼ |
| Enterokinase (enteropeptidase), light chain | P8070 | Asp-Asp-Asp-Asp-Lys▼ |
| Furin | P8077 | Arg-X-X-Arg▼ |
| Proteinase K | P8107 | non-specific, subtilisin-related serine protease |
| Thermolabile Proteinase K | P8111 | non-specific, subtilisin-related serine protease |
| Trypsin-ultra™, Mass Spectrometry Grade (Recombinant) | P8119 | Lys/Arg▼-X-X-X |
| α-Lytic Protease | P8113 | XX-T/A/S/V▼XX |
| Endoproteinase GluC | P8100 | Glu▼-X-X-X or Asp▼-X-X-X |
| Endoproteinase AspN | P8104 | X-X-X▼-Asp |
| Endoproteinase LysC | P8109 | X-X-Lys▼-X-X-X |
| TEV Protease | P8112 | ENLYFQ▼(S/G/A/M/C/H) |
| Cold-Active TEV Protease | P8118 | ENLYFQ▼(S/G/A/M/C/H/F) |
