| AatII | AsiSI | BsmBI-v2 | EagI | MboI | NlaIII | PvuI | SmaI |
| Acil | AvrII | BspHI | EcoRI | MboII | NotI | RsaI | SpeI |
| AfeI | BamHI | BsrGI | EcoRV | MluI | NspI | SacI | StuI |
| AflII | BbsI | ClaI | Esp3I | FseI | MseI | PacI | SacII |
| AgeI | BglII | DdeI | HaeIII | MspI | PciI | SalI | XbaI |
| AluI | BsaI | DpnI | HindIII | NcoI | PmeI | SapI | XhoI |
| ApaI | BseYI | DpnII | HpaI | NdeI | PsiI-v2 | SfaNI | XmaI |
| ApeKI | BsiWI | DraI | KpnI | NheI | PstI | SfiI | XmnI |
| AscI |
* All HF restriction enzymes are also supplied with Gel Loading Dye, Purple (6X)