FAQ: Is this Taq DNA Polymerase product compatible with other NEB Buffers?

Product name Hot Start TaqDNA Polymerase TaqDNA Polymerase with Standard Buffer Taq DNA Polymerase with Standard Buffer (Mg free) TaqDNA Polymerase with ThermoPol Buffer EpiMark® Hot Start TaqDNA Polymerase
Product number M0495 M0273 M0320 M0267 M0490
Supplied buffer Standard Taq Reaction Buffer Standard Taq Reaction Buffer Standard Taq (Mg-free) Reaction Buffer with 25mM MgCl2 ThermoPol® Reaction Buffer EpiMark® Hot Start Taq
Supplied buffer composition at 1X
(final reaction concentration)

10 mM Tris-HCl 50 mM KCl
1.5 mM MgCl2
(pH 8.3 @ 25°C)
10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl2
pH 8.3@25°C
0 mM Tris-HCl
50 mM KCl
pH 8.3@25°C
20 mM Tris-HCl 10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton ® X-100
pH 8.8@25°C
20 mM Tris-HCl
1.8 mM MgCl2
22 mM KCl
0.06% IGEPAL® CA-630
0.05% Tween ® 20
22 mM NH4Cl
(pH 8.9 @ 25°C)
Other compatible buffers

*Use in rCutSmart™ or otherNEBuffers is not recommended*
  • ThermoPol® Reaction Buffer
  • OneTaq ® Standard Reaction Buffer
  • LongAmp® TaqReaction Buffer
  • ThermoPol® Reaction Buffer

  • OneTaq® Standard Reaction Buffer
  • LongAmp® TaqReaction Buffer

  • Standard Taq Reaction Buffer
  • OneTaq® Standard Reaction Buffer
  • LongAmp® Taq Reaction Buffer
  • Standard Taq Reaction Buffer
  • One® Standard Reaction Buffer
  • LongAmp® Taq Reaction Buffer
  • ThermoPol® Reaction Buffer
  • Standard TaqReaction Buffer