FAQ: Can RNase inhibitor be added to mRNA Cap N6 Methyltransferase (m6A) (NEB #M0767) reactions?

Yes, RNase Inhibitor can be added to mRNA Cap N6 Methyltransferase (m6A) (NEB #M0767) reactions. The use of RNase Inhibitor is recommended to enhance RNA stability in the reaction. Add RNase Inhibitor (e.g., Murine RNase Inhibitor NEB #M0314) to the reaction to a final concentration of 1 unit/µl. If denaturing RNA at 65°C for 5 min, add RNase inhibitor after this step when the RNA is cooled.