FAQ: Can I still make ARTIC-SARS-CoV-2 Libraries for Illumina without enzymatic fragmentation?

Yes. You will need the following kits to recapitulate our discontinued product E7650.

  • E7626 NEBNext® ARTIC SARS-CoV-2 RT-PCR Module
  • E7645 NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®
  • E6440 NEBNext® Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs)

Follow the E7650 manual just replacing the NEBNext Library PCR Master Mix with NEBNext Ultra II Q5 Master Mix.