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Rapid Cell-Free Protein Expression Using PURExpress® and NEBExpress®

Synthesize peptide and protein targets in vitro, in hours instead of days

Expedite your next discovery using these complementary platforms – both ideal for early studies at scale using high-throughput technologies. Use plasmid DNA, linear DNA, or mRNA as templates.

•   NEBExpress® Cell-free E. coli Protein Synthesis System yields robust expression of a wide variety of protein targets
•   PURExpress® In Vitro Protein Synthesis yields application-ready proteins, free of modification and degradation, with options to control ribosomes, release factors, and tRNA or amino acid modifications

Cell-free Expression and Rapid Analysis
of Single-Chain Antibody Fragments

Cell-free protein synthesis workflows are cost-effective for single-chain antibody fragment production. Learn about a fast DNA-to-Data workflow validated by NEB for efficient screening with a methodology that can be applied to antibody fragments or miniprotein binders.

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PURExpress® vs. NEBExpress® Application Chart
Find the cell-free approach for your application.
See Selection Chart
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Automated Cell-free Protein Expression and Purification for High-Throughput Screening
NEB scientists demonstrate a rapid workflow to automate cell-free protein synthesis and protein purification using a magnetic bead-based method, compatible with various automation platforms, enabling high-throughput screening and discovery.



Review Application Note
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Tailored PURExpress® Kits for Flexible Cell-Free Protein Expression
Greater control for your experiments, with pre-configured kits optimized for specialized applications, including mRNA & ribosome display, use of alternative RNA polymerases, translation studies & automated workflows.
Find your solution

Featured Products

Explore NEB's cell-free tool kit for rapid protein synthesis, functional genomics, proteomics, protein engineering and synthetic biology. Remove the cell culture bottlenecks and accelerate discovery with proven in vitro protein expression systems.

This content is covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

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