Luna® Universal qPCR & RT-qPCR
Make a simpler choice

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Lighting the way – Luna® Universal qPCR & RT-qPCR banner with luna moth

With so many qPCR and RT-qPCR options available on the market, product selection can sometimes be difficult. Factors such as sample type, detection method and instrument compatibility have to be considered when deciding which to choose. Luna products were designed to simplify selection and maximize flexibility. Furthermore, Luna products utilize convenient master mix formats and user-friendly protocols, ensuring your singleplex and multiplex qPCR and RT-qPCR experiments are successful, each and every time.

Figure 1: Find the right Luna product for your application

Infographic to help find the right Luna product for your application

The Luna reference dye supports universal instrument compatibility (High-ROX, Low-ROX, ROX-independent)

  • Quickly set up reactions using convenient master mixes and user-friendly protocols
  • Prevent carryover contamination by pre-treating with Antarctic Thermolabile UDG (NEB #M0372)
  • Eliminate pipetting errors with non-interfering, visible tracking dye

 

Figure 2: Enjoy the convenience & compatibility

Luna universal instrument compatibility: high-ROX, low-ROX, ROX-independent

Typical Luna qPCR amplification plots using various real-time instruments. As shown, Luna’s universal reference dye is compatible with high-ROX, low-ROX and ROX-independent instruments. Default instrument view is shown.

 

Figure 3: Visible tracking

Illustration of blue visible tracking dye in tubes and plate

 

Available products:

Luna Universal qPCR Master Mix

Dye-based qPCR for DNA and cDNA
Luna Universal Probe qPCR Master Mix

Probe-based qPCR for DNA and cDNA
Luna Universal One-Step RT-qPCR Kit

Dye-based one-step RT-qPCR for RNA
Luna Universal Probe One-Step RT-qPCR Kit

Probe-based one-step RT-qPCR for RNA
Luna Probe One-Step RT-qPCR 4X Mix with UDG

4X mix for probe-based one-step RT-qPCR
LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG

Single tube with blue tracking dye includes everything needed for first strand cDNA synthesis in two-step RT-qPCR workflows

Luna Direct Probe One-Step RT-qPCR 4X Mix with UDG

Supports direct probe-based one-step RT-qPCR from crude or purified RNA samples 

Luna Direct Probe qPCR 4X Master Mix with UDG

Supports direct probe-based qPCR from crude or purified DNA samples 
Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit

Flexible first-strand cDNA synthesis with user-selected primers
LunaScript RT SuperMix Kit

Single tube with blue tracking dye includes everything needed for first strand cDNA synthesis in two-step RT-qPCR workflows
LunaScript RT Master Mix Kit (Primer-free)

Flexible first-strand cDNA synthesis with user-selected primers
Luna Cell Ready One-Step RT-qPCR Kit

Cell lysis module + dye-based RT-qPCR kit for direct detection from cell lysates
Luna Cell Ready Probe One-Step RT-qPCR Kit

Cell lysis module + probe-based RT-qPCR kit for direct detection from cell lysates
Luna Cell Ready Lysis Module

Cell lysis module alone

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Videos

  • Overview of qPCR

    Learn the basics of qPCR in this short animation.                                                                                                  

  • NEB® TV Ep. 16 – Overview and Applications of qPCR

    Learn the basics of qPCR, and hear more about our Luna products, including our novel method for data analysis.

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

 APPLIED BIOSYSTEMS™ is a trademark of Thermo Fisher Scientific.
STEPONEPLUS®and QUANTSTUDIO®are registered trademarks of Thermo Fisher Scientific.
CFX96 TOUCH™ is a trademark of Bio-Rad Laboratories, Inc.
BIO-RAD® is a registered trademark of Bio-Rad Laboratories, Inc.